ELISA Kit DIY Materials for Ghrelin (GHRL) Mus musculus (Mouse) Assay Kit DIY Materials

MTLRP; Growth Hormone-Releasing Peptide

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Overview
Properties
  • Reagent Contents Capture Antibody, Biotin-labeled Competitor, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable SampleSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
  • Applicable PrincipleCompetitive Inhibition ELISA for Antigen Detection
  • Detectable Range123.46-10000pg/mL
  • Applicable Sensitivity51.3pg/mL
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  • ELISA Kit DIY Materials for Ghrelin (GHRL) Packages (Simulation)
  • ELISA Kit DIY Materials for Ghrelin (GHRL) Packages (Simulation)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The Abs in the kit have high sensitivity and excellent specificity for detection of Ghrelin (GHRL). No significant cross-reactivity or interference between Ghrelin (GHRL) and analogues was observed.

Usage

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

Storage

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

Support pack

Citations

  • Expression of ghrelin in human salivary glands and its levels in saliva and serum in Chinese obese children and adolescentsPubMed: 21075359
  • Presence of adropin, nesfatin-1, apelin-12, ghrelins and salusins peptides in the milk, cheese whey and plasma of dairy cowsScienceDirect: S0196978113000533
  • Postnatal administration of 2-oxoglutaric acid improves articular and growth plate cartilages and bone tissue morphology in pigs prenatally treated with dexamethasonePubMed: 23211309
  • The modifying effects of fish oil on fasting ghrelin mRNA expression in weaned ratsPubMed: 22842192
  • Changes in Ghrelin and Obestatin Levels before and after a Meal in Children with Simple Obesity and AnorexiaPubmed: 23774845
  • Gastric supply manipulation to modulate ghrelin production and enhance vascularization to the cardia: proof of the concept in a porcine modelPubmed:25294792
  • Embolization of Arterial Gastric Supply in Obesity (EMBARGO): an Endovascular Approach in the Management of Morbid Obesity. Proof of the Concept in the Porcine ModelPubmed:25511751
  • Effect of Sleeve Gastrectomy Plus Side-to-Side Jejunoileal Anastomosis for Type 2 Diabetes Control in an Obese Rat ModelPubMed: 26202420
  • Gastric Supply Manipulation to Modulate Ghrelin Production and Enhance Vascularization to the Cardia Proof of the Concept in a Porcine ModelPubMed: 25294792
  • Elevation of Fasting Ghrelin in Healthy Human Subjects Consuming a High-Salt Diet: A Novel Mechanism of Obesity?Pubmed:27240398
  • Effects of sleeve gastrectomy with jejuno-jejunal or jejuno-ileal loop onglycolipid metabolism in diabetic rats.pubmed:27621579
  • Increased serum ghrelin in preeclampsia: is ghrelin a friend or a foe?pubmed:27321099
  • The association between serum ghrelin levels and large-for-gestational-age fetuses in patients with gestational diabetes mellituspubmed:27791436
  • Effect of lifestyle improvement program on the biomarkersof adiposity, inflammation and gut hormones in overweight/obese Asian Indians with prediabetespubmed:28620678
  • Role of serotonin hormone in weight regain after sleeve gastrectomy pubmed:29228802
  • EFFECT OF Garcinia cambogia EXTRACT ON SERUM LEPTIN, GHRELIN, ADIPONECTIN AND INSULIN LEVELS AND BODY WEIGHTS IN RATS FED WITH HIGH …Slovetres:Source
  • The Relations Among Serum Ghrelin, Motilin and Gastric Emptying and Autonomic Function in Autoimmune GastritisPubmed:29753372
  • The Role of Umbilical Cord Blood Concentration of IGF-I, IGF-II, Leptin, Adiponectin, Ghrelin, Resistin, and Visfatin in Fetal GrowthPubmed: 30282106
  • The reparative effects of human adipose-derived mesenchymal stem cells in the chemotherapy damaged thymusPubmed: 30511904
  • Grape Seed Proanthocyanidin Affects Lipid Metabolism via Changing Gut Microflora and Enhancing Propionate Production in Weaned PigsPubmed: 31175811
  • Associations of apelin, leptin, irisin, ghrelin, insulin, glucose levels, and lipid parameters with physical activity during eight weeks of regular exercise trainingPubmed: 31290696
  • Ghrelin system is involved in improvements in glucose metabolism mediated by hyperbaric oxygen treatment in a streptozotocin‑induced type 1 diabetes mouse …Pubmed: 32901885
  • Omega-3 offers better hypothalamus protection by decreasing POMC expression and elevating ghrelin hormone: a prospective trial to overcome methotrexate …Pubmed: 32424681
  • Serum leptin level is associated with phase angle in CKD5 patients not undergoing dialysisPubmed: 30089154
  • Physiological and Histopathological Effects of Electroporation Pulse on Stomach of Rats
  • Effect of Nutritional Supplementation on Treatment of Anoestrus Swamp Buffalo Cows and Heifers
  • Relationship Between Orexin-A and Insulin Resistance in Patients with Type 2 Diabetes Mellitus
  • Altered Serum Marker of Adipokines Profile in Breast Cancer Women
  • The Association of Pre-Pregnancy BMI On Leptin, Ghrelin, Adiponectin, and IGF-1 In Breast Milk, A Case Control Study34348809
  • Nutritional Status, Selected Nutrients Intake and Their Relationship with the Concentration of Ghrelin and Adiponectin in Patients with Diabetic Nephropathy34959967
  • Effect of Sleeve Gastrectomy, Roux-En-Y Gastric Bypass, and Ileal Transposition on Myocardial Ischaemia–Reperfusion Injury in Non-Obese Non-Diabetic Rats34903800
  • The microbiota-gut-kidney axis mediates host osmoregulation in a small desert mammalPubmed:35379849
  • Creating texturally tuneable, low calorie and palatable noodle-like food assemblies via microfluidics

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