ELISA Kit DIY Materials for Gibberellic Acid (GA)
GA3; Gibberellin A3; Gibberellins
- UOM
- FOB US$ 1,433.00 US$ 2,388.00 US$ 4,298.00 US$ 8,358.00 US$ 14,328.00
- Quantity
Overview
Properties
- Product No.KSA759Ge11
- Organism SpeciesPan-species (General) Same name, Different species.
- ApplicationsMain materials for "Do It (ELISA Kit) Yourself".
Research use only - Downloadn/a
- CategoryNutrition metabolismHormone metabolism
- Reagent Contents Capture Antibody, Biotin-labeled Competitor, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
- Detectable Sampletissue or cell culture supernates
- Applicable PrincipleCompetitive Inhibition ELISA for Antigen Detection
- Detectable Range123.5-10000ng/mL
- Applicable Sensitivity47.2ng/mL
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Specifity
The Abs in the kit have high sensitivity and excellent specificity for detection of Gibberellic Acid (GA). No significant cross-reactivity or interference between Gibberellic Acid (GA) and analogues was observed.
Usage
1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.
Storage
Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.
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Citations
- CRK5 Protein Kinase Contributes to the Progression of Embryogenesis of Arabidopsis thalianaPubmed: 31817249
- Functional analysis of the CDPK-Related Kinase (CRK) family in Arabidopsis thaliana
- The gibberellic acid derived from the plastidial MEP pathway is involved in the accumulation of Bamboo mosaic virusPubmed:35524450