ELISA Kit DIY Materials for Glucagon Like Peptide 1 (GLP1) Rattus norvegicus (Rat) Assay Kit DIY Materials

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Overview
Properties
  • Reagent Contents Capture Antibody, Biotin-labeled Competitor, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable SampleSerum, plasma and other biological fluids.
  • Applicable PrincipleCompetitive Inhibition ELISA for Antigen Detection
  • Detectable Range12.35-1000pg/mL
  • Applicable Sensitivity4.67pg/mL
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  • ELISA Kit DIY Materials for Glucagon Like Peptide 1 (GLP1) Packages (Simulation)
  • ELISA Kit DIY Materials for Glucagon Like Peptide 1 (GLP1) Packages (Simulation)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The Abs in the kit have high sensitivity and excellent specificity for detection of Glucagon Like Peptide 1 (GLP1). No significant cross-reactivity or interference between Glucagon Like Peptide 1 (GLP1) and analogues was observed.

Usage

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

Storage

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

Support pack

Citations

  • Specificity and sensitivity of commercially available assays for glucagon‐like peptide‐1 (GLP‐1): implications for GLP‐1 measurements in clinical studiesPubmed:25041349
  • Expression of cholecystokinin2-receptor in rat and human L cells and the stimulation of glucagon-like peptide-1 secretion by gastrin treatmentPubmed:25601282
  • Inhibitory effect of somatostatin on insulin secretion is not mediated via the CNSPubmed:25630331
  • Effect of Sleeve Gastrectomy Plus Side-to-Side Jejunoileal Anastomosis for Type 2 Diabetes Control in an Obese Rat ModelPubMed: 26202420
  • Anti-hiperglycemic Effect of Urena lobata Leaf Extract by Inhibition of Dipeptidyl Peptidase IV (DPP-IV) on Diabetic RatsPdf: 7
  • Subcutaneous administration of liraglutide ameliorates learning and memory impairment by modulating tau hyperphosphorylation via the glycogen synthase kinase-3尾 pathway in an amyloid 尾 protein induced alzheimer disease mouse modelPubmed:27131827
  • miR-200a reCavia (Guinea pig )lates Rheb-mediated amelioration of insulin resistance after duodenal-jejunal bypassPubmed:27121251
  • Incretin effect of Urena lobata leaves extract on structure and function of rats islet β-cellsS2225411016302802
  • Alterations in gut microbiota during remission and recurrence of diabetes after duodenal-jejunal bypass in ratspubmed:27547013
  • Effects of sleeve gastrectomy with jejuno-jejunal or jejuno-ileal loop onglycolipid metabolism in diabetic rats.pubmed:27621579
  • Improvement in glucose tolerance and insulin sensitivity by probiotic strains of Indian gut originin high-fat diet-fed C57BL/6J mice.pubmed:27757592
  • Incretin effect of Urena lobata leaves extract on structure and function of rats islet b-cellsd69b5594c032d557c3f3203b91aba2604a94
  • Deactivation of the NLRP3 inflammasome in infiltrating macrophages by duodenal-jejunal bypass surgery mediates improvement of beta cell function in type 2 diabetes10.1016/j.metabol.2017.10.015
  • The Effects of Duodenojejunal Omega Switch in Combination with High-Fat Diet and Control Diet on Incretins, Body Weight, and Glucose Tolerance in Sprague-Dawley Rats.pubmed:28840471
  • Selective Vagotomy Worsens Glucose Control After Ileal TranspositionPubmed:29525935
  • Preserving Duodenal-Jejunal (Foregut) Transit Does Not Impair Glucose Tolerance and Diabetes Remission Following Gastric Bypass in Type 2 Diabetes Sprague …Pubmed:29098544
  • Ileal Transposition Surgery Decreases Fat Mass and Improves Glucose Metabolism in Diabetic GK Rats: Possible Involvement of FGF21Pubmed:29593555
  • Downregulation of lncRNA MALAT1 contributes to renal functional improvement after duodenal-jejunal bypass in a diabetic rat modelPubmed:29781038
  • Deactivation of the NLRP3 inflammasome in infiltrating macrophages by duodenal-jejunal bypass surgery mediates improvement of beta cell function in type 2 …Pubmed:29129820
  • Effects of Diet-Induced Early-Stage Obesity on a Low-Testosterone Gottingen Minipig
  • The influence of high fat diet on plasma incretins and insulin concentrations in Sprague-Dawley rats with diet-induced obesity and glucose intolerance undergoing …Doi: 10.1016/j.peptides.2019.04.001
  • Grape Seed Proanthocyanidin Affects Lipid Metabolism via Changing Gut Microflora and Enhancing Propionate Production in Weaned PigsPubmed: 31175811
  • Alcohol Withdrawal and Proopiomelanocortin Neuropeptides in an Animal Model of Alcohol DependencePubmed: 31117084
  • The Leading Role of Peptide Tyrosine Tyrosine in Glycemic Control After Roux-en-Y Gastric Bypass in RatsPubmed: 31701411
  • Novel GPR120 agonist TUG891 modulates fat taste perception and preference and activates tongue-brain-gut axis in micePubmed: 31806728
  • Metabolik sendromlu sıçanlarda yüzme egzersizinin plazma glukagon-benzeri PEPTİD-1 (GLP-1) seviyesi ve hipokampal GLP-1 reseptörü üzerine etkisinin …
  • Effect of oligofructose on resistance to postoperative high-fat diet-induced damage of metabolism in diabetic rats after sleeve gastrectomy33889290
  • Study on the Therapeutic Effects of Traditional Chinese Medicine on Insulin Resistance in Obese Mice by Modulating Intestinal Function
  • Central GLP-1 contributes to improved cognitive function and brain glucose uptake after duodenum-jejunum bypass on obese and diabetic rats34370593
  • The effect of bariatric surgery on the expression of Growth Differentiation Factor-15/Macrophage-Inhibitory Cytokine-1 (GDF-15/MIC-1) in ratPubmed:35029766
  • The novel adamantane derivatives as potential mediators of inflammation and neural plasticity in diabetes mice with cognitive impairmentPubmed:35468904
  • Response of blood glucose and GLP-1 to different food temperature in normal subject and patients with type 2 diabetesPubmed:35624116
  • Different Estradiol Level's Impact on Appetite Regulation, Food Intake, and Concentrations of Leptin, Glucagon-Like Peptide-1, and High-Sensitivity C-Reactive …

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