ELISA Kit DIY Materials for Glucose Transporter 4 (GLUT4)

SLC2A4; Solute carrier family 2, facilitated glucose transporter member 4; Glucose transporter type 4, insulin-responsive

UOM
1. 96T*5
2. 96T*10
3. 96T*20
4. 96T*50
5. 96T*100
FOB US$ 1,368.00 US$ 2,280.00 US$ 4,104.00 US$ 7,980.00 US$ 13,680.00
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Overview

Properties

Product No.KSC023Ra01
Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Canine
- Porcine
- Bovine
- Caprine
- Multi-species
- Pan-species
- Others
ApplicationsMain materials for "Do It(ELISA Kit) Yourself"
Research use only
Downloadn/a
CategorySignal transduction Metabolic pathway Endocrinology Cardiovascular biology

Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
Detectable SampleTissue homogenates, cell lysates and other biological fluids.
Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
Detectable Range0.625-40ng/mL
Applicable Sensitivity0.272ng/mL

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Packages (Simulation)
Packages (Simulation)

ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The Abs in the kit have high sensitivity and excellent specificity for detection of Glucose Transporter 4 (GLUT4). No significant cross-reactivity or interference between Glucose Transporter 4 (GLUT4) and analogues was observed.

Usage

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

Storage

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

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ELISA / CLIA Experiment Service

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Citations

Glucose transporter-4 in white blood cells of young and old sled dogs: a model for human biomarker developmentCambridge: Source
Conditioning causes an increase in glucose transporter-4 levels in mononuclear cells in sled dogsPubmed:25236492
Maternal stress predicts altered biogenesis and the profile of mitochondrial proteins in the frontal cortex and hippocampus of adult offspring ratsPubMed: 26143539
Antidiabetic Effect of Galantamine: Novel Effect for a Known Centrally Acting DrugPubMed: 26262991
Metabolic Features of Heart Failure with Different Etiology ofsp11075
Hibiscus sabdariffa linn fruits methanolic extract and fractions mediated glucose uptake stimulation and glucose transporter 4 regulationDoi: 10.4103/jpnr.JPNR_14_18
Effect of Noni Fruit Extract (Morinda citrifolia) on Glucose Intake to Diabetes Mellitus White Rat Muscle Tissue
The Effects of Sambiloto Leaf Extract (Andrographis peniculata) on Blood Sugar Regulation: an In Vivo Study
Effect of Bay Leaf Extract (Syzygium polyanthum) on Blood Sugar Regulation via GLUT4 Protein Regulation in White Rat Muscle Tissue Induced Aloxan
Potential Combination of Tinospora crispa, Andrographis paniculata, Cinamomum burmanii, Syzygium polyanthum and Momordica charantia Extracts Against?¡