ELISA Kit DIY Materials for Glutathione (GSH) Pan-species (General) Assay Kit DIY Materials

L-Reduced Glutathione

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Overview
Properties
  • Reagent Contents Capture Antibody, Biotin-labeled Competitor, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable Sampleserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
  • Applicable PrincipleCompetitive Inhibition ELISA for Antigen Detection
  • Detectable Range1.23-100ug/mL
  • Applicable Sensitivity0.48ug/mL
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  • ELISA Kit DIY Materials for Glutathione (GSH) Packages (Simulation)
  • ELISA Kit DIY Materials for Glutathione (GSH) Packages (Simulation)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The Abs in the kit have high sensitivity and excellent specificity for detection of Glutathione (GSH). No significant cross-reactivity or interference between Glutathione (GSH) and analogues was observed.

Usage

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

Storage

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

Support pack

Citations

  • Evidence for augmented oxidative stress in the subjects with type 1 diabetes and their siblings: a possible preventive role for antioxidantsPubMed: 22781023
  • Glutathione S-Transferase P1 Correlated with Oxidative Stress in Hepatocellular CarcinomaPubMed: PMC3619117
  • Alteration in the intrafollicular thiol–redox system in infertile women with endometriosisPubmed:25376627
  • Effects of mild hypothermia therapy on the levels of glutathione in rabbit blood and cerebrospinal fluid after cardiopulmonary resuscitationPubMed: 25810895
  • Oxidative Stability of Ostrich Meat Related to Duration of Linseed and Lucerne Supplementation to the Bird's DietView: J
  • Prenatal maternal distress affects atopic dermatitis in offspring mediated by oxidative stressPubmed:27016803
  • Hydrogen sulfide protects against chronic unpredictable mild stress-induced oxidative stress in hippocampus by upreCavia (Guinea pig )lation of BDNF-TrkB pathwayjournals:2153745.pdf
  • Rapid opioid detoxification with an implementation of the new method of gradually increasing dosage of naltrexone inductionobject:elaba:15733325
  • Toxicity study of oxalicumone A, derived from a marine-derived fungus Penicillium oxalicum, in cultured renal epithelial cellspubmed:28260084
  • Hepatocellular toxicity of oxalicumone A via oxidative stress injury and mitochondrial dysfunction in healthy human liver cellspubmed:29115483
  • Metabolomic and transcriptomic profiling of hepatocellular carcinomas in Hras12V transgenic micepubmed:28941178
  • Circulating cell-free DNA of methylated insulin-like growth factor-binding protein 7 predicts a poor prognosis in hepatitis B virus-associated hepatocellular carcinoma …Pubmed:29463155
  • Epigallocatechin-3-Gallate Reduces Neuronal Apoptosis in Rats after Middle Cerebral Artery Occlusion Injury via PI3K/AKT/eNOS Signaling PathwayPubmed:29770336
  • Epigallocatechingallate attenuates myocardial injury in a mouse model of heart failure through TGF‑β1/Smad3 signaling pathwayPubmed:29620209
  • Effects of ginsenoside Rb1 on oxidative stress injury in rat spinal cords by regulating the eNOS/Nrf2/HO‑1 signaling pathwayPubmed:30116359
  • Effects of Tualang honey in modulating nociceptive responses at the spinal cord in offspring of prenatally stressed ratsDoi: 10.1016/j.joim.2018.12.002
  • Protective effects of sulforaphane on diabetic retinopathy: activation of the Nrf2 pathway and inhibition of NLRP3 inflammasome formationPubmed: 30606939
  • Porous Se@ SiO2 nanosphere-coated catheter accelerates prostatic urethra wound healing by modulating macrophage polarization through reactive oxygen species …Pubmed: 30753941
  • MicroRNA‑21 contributes to the puerarin‑induced cardioprotection via suppression of apoptosis and oxidative stress in a cell model of ischemia/reperfusion injuryPubmed: 31115556
  • Germinated-soy milk as a healthy diet to induce high antioxidant enzymes in breast milk
  • Hydrogen Sulfide Prevents Sleep Deprivation-Induced Hippocampal Damage by Upregulation of Sirt1 in the HippocampusPubmed: 32218719
  • Dexmedetomidine Attenuates Orthotopic Liver Transplantation-Induced Acute Gut Injury via α2-Adrenergic Receptor-Dependent Suppression of Oxidative StressPubmed: 31827710
  • Exosomes of Antler Mesenchymal Stem Cells Improve Postoperative Cognitive Dysfunction in Cardiopulmonary Bypass Rats through Inhibiting the TLR2 …Pubmed: 32300366
  • Antiepileptic effects of exogenous β‑hydroxybutyrate on kainic acid‑induced epilepsyPubmed: 33101467
  • Association Between Serum Selenium Level and Subclinical Mastitis in Dairy CattlePubmed: 32583225
  • Formaldehyde induces ferroptosis in hippocampal neuronal cells by upregulation of the Warburg effect33259821
  • Glutamine deficiency promotes stemness and chemoresistance in tumor cells through DRP1-induced mitochondrial fragmentation33895866
  • Naringenin alleviates myocardial ischemia reperfusion injury by enhancing the myocardial miR-126-PI3K/AKT axis in streptozotocin-induced diabetic rats34093766
  • MicroRNA-145-5p aggravates cell apoptosis and oxidative stress in tongue squamous cell carcinoma33732346
  • Oxidative stress-and mitochondrial dysfunction-mediated cytotoxicity by silica nanoparticle in lung epithelial cells from metabolomic perspective33662726
  • Lycium barbarum polysaccharides attenuate cardiovascular oxidative stress injury by enhancing the Keap1/Nrf2 signaling pathway in exhaustive exercise rats34278476
  • κ-Opioid Receptor Agonist Ameliorates Postoperative Neurocognitive Disorder by Activating the Ca2+/CaMKII/CREB Pathway34608407
  • Alamandine alleviates hypertension and renal damage via oxidative-stress attenuation in Dahl ratsPubmed:35022384
  • Intake of Bifidobacterium lactis Probio-M8 fermented milk protects against alcoholic liver diseasePubmed:35086715
  • Antioxidant Effect of Tyr-Ala Extracted from Zein on INS-1 Cells and Type 2 Diabetes High-Fat-Diet-Induced MicePubmed:35740008
  • The κ-Opioid Receptor Agonist U50488H Ameliorates Neuropathic Pain Through the Ca2+/CaMKII/CREB Pathway in RatsPubmed:35645576

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