ELISA Kit DIY Materials for Growth Hormone (GH) Rattus norvegicus (Rat) Assay Kit DIY Materials

GH1; GH-N; GHN; hGH-N; Somatotropin; Hygetropin; Jintropin; Kigtropin; Pituitary Growth Hormone; Growth Hormone, Normal

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Overview
Properties
  • Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable SampleSerum, plasma and other biological fluids.
  • Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
  • Detectable Range0.156-10ng/mL
  • Applicable Sensitivity0.056ng/mL
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  • ELISA Kit DIY Materials for Growth Hormone (GH) Packages (Simulation)
  • ELISA Kit DIY Materials for Growth Hormone (GH) Packages (Simulation)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The Abs in the kit have high sensitivity and excellent specificity for detection of Growth Hormone (GH). No significant cross-reactivity or interference between Growth Hormone (GH) and analogues was observed.

Usage

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

Storage

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

Support pack

Citations

  • The effect of peripheral administration of growth hormone on AD-like cognitive deficiency in NBM-lesioned ratsPubMed: 19765635
  • Changes in the Somatotrophic Axis in Genetically Identical DogsPubMed: 21740269
  • The efficacy of IGF-I receptor monoclonal antibody against human gastrointestinal carcinomas is independent of k-ras mutation statusPubMed: 21642381
  • Growth hormone expression and neuroprotective activity in a quail neural retina cell lineScienceDirect: S0016648009002457
  • Periodic 48 h feed withdrawal improves glucose tolerance in growing pigs by enhancing adipogenesis and lipogenesisNutrition&Metabolism: source
  • Treatment of cervical metaphyseal fractures due to delayed ossification in an adult dog with hypopituitarismBmj: Source
  • GH Deficiency in a Dalmatian Puppy with MegaesophagusPubmed: 24051259
  • Functional expression of bovine growth hormone gene in Pleurotus eryngiiSpringer: Source
  • Profil Growth Hormone Sapi Bali Betina Pada Tiga Tipe Lahan Pemeliharaan Di Provinsi BaliUnud:Source
  • Effect of prill fat supplementation on hormones, milk production and energy metabolites during mid lactation in crossbred cowsVeterinaryworld:Source
  • Effects of sericin on the testicular growth hormone/insulin-like growth factor-1 axis in a rat model of type 2 diabetesPubMed: 26379831
  • Melatonin modulates monochromatic light-induced GHRH expression in the hypothalamus and GH secretion in chicksPubmed:26948666
  • Comparison of the effect of a standard inclusion level of inorganic zinc to organic form at lowered level on bone development in growing male Ross broiler chickens journalprintahead:87
  • Comparison of the Effect of a Standard Inclusion Level of Inorganic Zinc to Organic Form at Lowered Level on Bone Development in Growing Male Ross Broiler Chickensview:87
  • Effect of thermal stress on physiological, hormonal and haematological parameters in Tharparkar and Karan Fries calvespublication:306322503
  • TRANSMISI GEN PhGH DAN PERFORMA PERTUMBUHAN IKAN LELE AFRIKA (Clarias gariepinus) TRANSGENIK GENERASI KETIGA1329
  • Assessment of feeding varying levels of Metabolizable energy and protein on performance of transition Murrah buffaloespubmed:28785907
  • Effect of Dietary Phytase Supplementation on Bone and Hyaline Cartilage Development of Broilers Fed with Organically Complexed Copper in a Cu-Deficient Dietarticle:10.1007
  • Effect of Zinc Level and Source (Zinc Oxide Vs. Zinc Glycine) on Bone Mechanical and Geometric Parameters, and Histomorphology in Male Ross 308 Broiler Chicken10.159:1806-9061-2016-0285
  • 单色绿光对鸡胚褪黑激素合成的影响DOI: 10.11843/j.issn.0366-6964.2017.05.019
  • Effects of Bariatric Surgery on Change of Brown Adipocyte Tissue and Energy Metabolism in Obese Micepubmed:28853013
  • Kadar Hormon Pertumbuhan Sapi Bali Lebih Rendah di Nusa Penida Daripada Daerah Bali Lainnya(LEVELS OF GROWTH HORMONE BALI CATTLE IN NUSA PENIDA LOWER THAN OTHER BALI REGIONS)10.19087/jveteriner.2017.18.2.226
  • Factors influencing growth hormone levels of Bali cattle in Bali, Nusa Penida, and Sumbawa Islands, Indonesia.pubmed:29184372
  • Effects of Dietary Lysine Levels on the Plasma Concentrations of Growth‐Related Hormones in Late‐Stage Finishing Pigs10.5772/intechopen.68545
  • Decreased Hepatic Lactotransferrin Induces Hepatic Steatosis in Chronic Non-Alcoholic Fatty Liver Disease ModelPubmed:29975946
  • Subsequent somatic axis and bone tissue metabolism responses to a low-zinc diet with or without phytase inclusion in broiler chickensPubmed:29373588
  • Effects of Intrauterine Growth Retardation on Growth, Meat Quality and Muscle Fiber Composition of Pigs.:
  • Zika Virus Infection in Hypothalamus Causes Hormone Deficiencies and Leads to Irreversible Growth Delay and Memory Impairment in MicePubmed: 30404008
  • Physiological Performance of Crossbred Cattle Calves (Karan Fries) under Different Housing Conditions during Different Seasons
  • Melatonin Receptor Mel1b‐and Mel1c‐mediated Green Light Induced the Secretion of Growth Hormone in Anterior Pituitary of ChicksPubmed: 31111492
  • Dietary lysine affects amino acid metabolism and growth performance, which may not involve the GH/IGF-1 axis, in young growing pigsPubmed: 31922564
  • The evaluation of leukocyte-platelet rich fibrin as an anti-inflammatory autologous biological additivePubmed: 31287125
  • RFRP-3, the Mammalian Ortholog of GnIH, Is a Novel Modulator Involved in Food Intake and Glucose HomeostasisPubmed: 32328034
  • Isolated growth hormone deficiency in a Chihuahua with a GH1 mutationPubmed: 32646299
  • Controlled elevated temperatures during early-mid gestation cause placental insufficiency and implications for fetal growth in pregnant pigs33244103
  • Effect of alfalfa hay and starter feed supplementation on caecal microbiota and fermentation, growth, and health of yak calves33610512
  • GHR is involved in gastric cell growth and apoptosis via PI3K/AKT signalling33492754
  • Effects of Concentrate and Bypass Fat Supplementations on Growth Performance, Blood Profile, and Rearing Cost of Feedlot Buffaloes34359233

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