ELISA Kit DIY Materials for Growth Hormone Releasing Hormone (GHRH)

GRF; GHRF; Somatocrinin; Growth-Hormone-Releasing Factor; Sermorelin; Somatoliberin

UOM
1. 96T*5
2. 96T*10
3. 96T*20
4. 96T*50
5. 96T*100
FOB US$ 1,134.00 US$ 1,890.00 US$ 3,402.00 US$ 6,615.00 US$ 11,340.00
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Overview

Properties

Product No.KSA438Hu11
Organism SpeciesHomo sapiens (Human) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Canine
- Porcine
- Bovine
- Caprine
- Multi-species
- Pan-species
- Others
ApplicationsMain materials for "Do It (ELISA Kit) Yourself".
Research use only
Downloadn/a
CategoryEndocrinology Hormone metabolism

Reagent Contents Capture Antibody, Biotin-labeled Competitor, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
Detectable SampleSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Applicable PrincipleCompetitive Inhibition ELISA for Antigen Detection
Detectable Range12.35-1000pg/mL
Applicable Sensitivity4.92pg/mL

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Packages (Simulation)
Packages (Simulation)

ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The Abs in the kit have high sensitivity and excellent specificity for detection of Growth Hormone Releasing Hormone (GHRH). No significant cross-reactivity or interference between Growth Hormone Releasing Hormone (GHRH) and analogues was observed.

Usage

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

Storage

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

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ELISA / CLIA Experiment Service

Increment services

Citations

Role of GHRH in Sleep and Growth Impairments Induced by Upper Airway Obstruction in RatsErs: 00197610
Role of growth hormone-releasing hormone in sleep and growth impairments induced by upper airway obstruction in ratsPubMed: 21406516
Effect of Moringa Oleifera Extract on Nicotine Induced Neurotoxicity in Female Rat and Their EmbryosIdosi: Source
Zika Virus Infection in Hypothalamus Causes Hormone Deficiencies and Leads to Irreversible Growth Delay and Memory Impairment in MicePubmed: 30404008
An Immuno‐PCR screen for the detection of CJC‐1295 and other Growth Hormone Releasing Hormone analogues in equine plasmaPubmed: 30489688
Effects of somatotropic axis on cognitive dysfunction of obstructive sleep apneaPubmed: 31073904
Lack of brain insulin receptor substrate-1 causes growth retardation, with decreased expression of growth hormone-releasing hormone in the hypothalamus.33980693
Associations between serum levels of brain-derived neurotrophic factor, corticotropin releasing hormone and mental distress in vitiligo patientsPubmed:35508633