ELISA Kit DIY Materials for Growth Hormone Releasing Hormone (GHRH)
GRF; GHRF; Somatocrinin; Growth-Hormone-Releasing Factor; Sermorelin; Somatoliberin
- UOM
- FOB US$ 1,166.00 US$ 1,944.00 US$ 3,499.00 US$ 6,804.00 US$ 11,664.00
- Quantity
Overview
Properties
- Product No.KSA438Mu11
- Organism SpeciesMus musculus (Mouse) Same name, Different species.
- ApplicationsMain materials for "Do It (ELISA Kit) Yourself".
Research use only - Downloadn/a
- CategoryEndocrinologyHormone metabolism
- Reagent Contents Capture Antibody, Biotin-labeled Competitor, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
- Detectable SampleSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
- Applicable PrincipleCompetitive Inhibition ELISA for Antigen Detection
- Detectable Range12.35-1000pg/mL
- Applicable Sensitivity4.63pg/mL
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Specifity
The Abs in the kit have high sensitivity and excellent specificity for detection of Growth Hormone Releasing Hormone (GHRH). No significant cross-reactivity or interference between Growth Hormone Releasing Hormone (GHRH) and analogues was observed.
Usage
1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.
Storage
Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.
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Citations
- Role of GHRH in Sleep and Growth Impairments Induced by Upper Airway Obstruction in RatsErs: 00197610
- Role of growth hormone-releasing hormone in sleep and growth impairments induced by upper airway obstruction in ratsPubMed: 21406516
- Effect of Moringa Oleifera Extract on Nicotine Induced Neurotoxicity in Female Rat and Their EmbryosIdosi: Source
- Zika Virus Infection in Hypothalamus Causes Hormone Deficiencies and Leads to Irreversible Growth Delay and Memory Impairment in MicePubmed: 30404008
- An Immuno‐PCR screen for the detection of CJC‐1295 and other Growth Hormone Releasing Hormone analogues in equine plasmaPubmed: 30489688
- Effects of somatotropic axis on cognitive dysfunction of obstructive sleep apneaPubmed: 31073904
- Lack of brain insulin receptor substrate-1 causes growth retardation, with decreased expression of growth hormone-releasing hormone in the hypothalamus.33980693
- Associations between serum levels of brain-derived neurotrophic factor, corticotropin releasing hormone and mental distress in vitiligo patientsPubmed:35508633