ELISA Kit DIY Materials for Heat Shock Protein 27 (Hsp27) Bos taurus; Bovine (Cattle) Assay Kit DIY Materials

HSPB1; HSP-B1; CMT2F; HSP28; Hsp25; Heat Shock 27kDa Protein 1; 28 kDa heat shock protein; Heat shock protein beta-1; Estrogen-regulated 24 kDa protein;

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Overview
Properties
  • Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable SampleSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
  • Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
  • Detectable Range31.2-2000pg/mL
  • Applicable Sensitivity13.2pg/mL
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  • ELISA Kit DIY Materials for Heat Shock Protein 27 (Hsp27) Packages (Simulation)
  • ELISA Kit DIY Materials for Heat Shock Protein 27 (Hsp27) Packages (Simulation)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The Abs in the kit have high sensitivity and excellent specificity for detection of Heat Shock 27kDa Protein 1 (HSPB1). No significant cross-reactivity or interference between Heat Shock 27kDa Protein 1 (HSPB1) and analogues was observed.

Usage

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

Storage

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

Support pack

Citations

  • The effect of heat stress on gene expression and synthesis of heat‐shock and milk proteins in bovine mammary epithelial cellsPubMed: 26467738
  • HSP27 is a partner of JAK2-STAT5 and a potential therapeutic target in myelofibrosisPubmed:29650953
  • Molecules of Damage-Associated Patterns in Bronchoalveolar Lavage Fluid and Serum in Chronic Obstructive Pulmonary DiseasePubmed:29429028
  • The beneficial effects of 15 units of high-intensity circuit training in women is modified by age, baseline insulin resistance and physical capacityPubmed: 31102684
  • Damage-Associated Molecular Patterns and Myeloid-Derived Suppressor Cells in Bronchoalveolar Lavage Fluid in Chronic Obstructive Pulmonary Disease Patients
  • Damage-Associated Molecular Patterns and Th-Cell-Related Cytokines Released after Progressive EffortPubmed: 32210109
  • Regulatory T cells, damage-associated molecular patterns, and myeloid-derived suppressor cells in bronchoalveolar lavage fluid interlinked with chronic obstructive …Pubmed:35687771
  • Immunization of mice with gold nanoparticles conjugated to thermostable tumor antigens prevents tumor development during transplantation

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