ELISA Kit DIY Materials for Hedgehog Homolog, Indian (IHH)
BDA1; HHG2
- UOM
- FOB US$ 1,368.00 US$ 2,280.00 US$ 4,104.00 US$ 7,980.00 US$ 13,680.00
- Quantity
Overview
Properties
- Product No.KSD116Ra01
- Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
- ApplicationsMain materials for "Do It (ELISA Kit) Yourself".
Research use only - Downloadn/a
- CategorySignal transduction
- Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
- Detectable SampleTissue homogenates, cell lysates and other biological fluids.
- Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
- Detectable Range0.156-10ng/mL
- Applicable Sensitivity0.062ng/mL
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Specifity
The Abs in the kit have high sensitivity and excellent specificity for detection of Hedgehog Homolog, Indian (IHH). No significant cross-reactivity or interference between Hedgehog Homolog, Indian (IHH) and analogues was observed.
Usage
1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.
Storage
Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.
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Citations
- Prostate cancer cells and bone stromal cells mutually interact with each other through bone morphogenetic protein-mediated signalsPubMed: 22532569
- Defective CFTR promotes intestinal proliferation via inhibition of the hedgehog pathway during cystic fibrosisPubmed: 30639531
- Eugenia Marbach-Breitrück, Madlen Matz-Soja, Ute Abraham, Wolfgang Schmidt-Heck, Susanne Sales, Christiane Rennert, Matthias Kern, Susanne Aleithe, Luise?…Pubmed: 30711403
- Tick-Tock hedgehog–mutual crosstalk with liver circadian clock renders hepatic hedgehog signaling a risk factor of steatosis
- MicroRNA-1 promotes cartilage matrix synthesis and regulates chondrocyte differentiation via post-transcriptional suppression of Ihh expressionPubmed: 32705199