ELISA Kit DIY Materials for Heme Oxygenase 1 (HO1) Sus scrofa; Porcine (Pig) Assay Kit DIY Materials

HMOX1; Hsp32; HMOX1D

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Overview
Properties
  • Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable SampleSerum, plasma, tissue homogenates, cell lysates and other biological fluids.
  • Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
  • Detectable Range0.312-20ng/mL
  • Applicable Sensitivity0.119ng/mL
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  • ELISA Kit DIY Materials for Heme Oxygenase 1 (HO1) Packages (Simulation)
  • ELISA Kit DIY Materials for Heme Oxygenase 1 (HO1) Packages (Simulation)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The Abs in the kit have high sensitivity and excellent specificity for detection of Heme Oxygenase 1, Decycling (HO1). No significant cross-reactivity or interference between Heme Oxygenase 1, Decycling (HO1) and analogues was observed.

Usage

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

Storage

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

Support pack

Citations

  • Characterization of the Intrarenal Renin-Angiotensin System in Experimental Alport SyndromePubMed: 25777063
  • Serum Heme Oxygenase-1 and BMP-7 Are Potential Biomarkers for Bone Metabolism in Patients with Rheumatoid Arthritis and Ankylosing SpondylitisPubmed:27314037
  • Circulating CD14+HLA-DR-/low myeloid-derived suppressor cells in leukemia patients with allogeneic hematopoietic stem cell transplantation: novel clinical potential strategies for the prevention and cellular therapy of graft-versus-host diseasePubmed:27109254
  • Decreased expression of heme oxygenase is associated with depressive symptoms and may contribute to depressive and hypertensive comorbidityPubmed:26824276
  • Sulforaphane protects against sodium valproate–induced acute liver injurypubmed:28112972
  • Carnosine ameliorates cognitive deficits in streptozotocin-induced diabetic rats: Possible involved mechanisms.pubmed:27777064
  • S-allyl cysteine ameliorates cognitive deficits in streptozotocin-diabetic rats via suppression of oxidative stress, inflammation, and acetylcholinesterase.pubmed:27887948
  • Ellagic acid exerts protective effect in intrastriatal 6-hydroxydopamine rat model of Parkinson's disease: Possible involvement of ERβ/Nrf2/HO-1 signaling.pubmed:28238669
  • Citrus leaf extract reduces blood pressure and vascular damage in repeatedly heated palm oil diet-Induced hypertensive rats.pubmed:28068636
  • Implication of Nrf2/HO-1 pathway in the coloprotective effect of coenzyme Q10 against experimentally induced ulcerative colitis.pubmed:28050757
  • Ameliorative potential of linagliptin and/or calcipotriol on bleomycin-induced lung fibrosis: In vivo and in vitro studypubmed:28192751
  • Identification of a panel of serum protein markers in early stage of sepsis and its validation in a cohort of patientspubmed:28655573
  • Hepatic, adipocyte, enteric and pancreatic hormones: response to dietary macronutrient composition and relationship with metabolismpubmed:28694840
  • Ameliorative potential of sitagliptin and/or resveratrol on experimentallyinduced clear cell renal cell carcinomapubmed:29101811
  • Serum complement factor 5a levels are associated with nonalcoholic fatty liver disease in obese childrenDOI: 10.1111/apa.14106
  • Quercetin and tin protoporphyrin attenuate hepatic ischemia reperfusion injury: role of HO-1pubmed:28589248
  • Ameliorative Effect of Coenzyme Q10 and/or Candesartan on Carboplatin-Induced Nephrotoxicity: Roles of Apoptosis, Transforming Growth Factor-Β1, Nuclear Factor Kappa-B And The Nrf2/HO-1 Pathwaypubmed:28670881
  • Berberine ameliorates intrahippocampal kainate-induced status epilepticus and consequent epileptogenic process in the rat: Underlying mechanismspubmed:28061403
  • Simultaneous accurate quantification of HO-1, CD39, and CD73 in human calcified aortic valves using multiple enzyme digestion–filter aided sample pretreatment …10.1016:j.talanta.2018.01.044
  • The Association between Inflammatory Markers (iNOS, HO-1, IL-33, MIP-1β) and Depression with and without Posttraumatic Stress Disorder10.1016:j.pharep.2018.06.001
  • Linagliptin potentiates the effect of l-dopa on the behavioural, biochemical and immunohistochemical changes in experimentally-induced Parkinsonism: Role of toll …Pubmed:29410018
  • Targeting the proinflammatory cytokines, oxidative stress, apoptosis and TGF-β1/STAT-3 signaling by irbesartan to ameliorate doxorubicin-induced hepatotoxicityPubmed:29753615
  • Anti-hemolytic and anti-inflammatory activities of the methanolic extract of (P.Beauv.) Briq. leaves in 2-butoxyethanol-hemolytic induced …10.1063:1.5033415
  • Tempol relieves lung injury in a rat model of chronic intermittent hypoxia via suppression of inflammation and oxidative stressDoi: 10.22038/IJBMS.2018.31716.7714
  • Targeting Nrf2/HO-1 signaling by crocin: Role in attenuation of AA-induced ulcerative colitis in ratsPubmed: 30530041
  • Sodium arsenite augments sensitivity of Echinococcus granulosus protoscoleces to albendazolePubmed: 30790573
  • Cilostazol against 2, 4, 6‐trinitrobenzene sulfonic acid‐induced colitis: Effect on tight junction, inflammation, and apoptosis
  • Vanillic acid alleviates palmitic acid‐induced oxidative stress in human umbilical vein endothelial cells via Adenosine Monophosphate‐Activated Protein Kinase …
  • Effect of heme oxygenase 1 and renin/prorenin receptor on oxidized low‑density lipoprotein‑induced human umbilical vein endothelial cells
  • Crocin attenuates lung inflammation and pulmonary vascular dysfunction in a rat model of bleomycin-induced pulmonary fibrosisPubmed: 31465731
  • Targeting oxidative stress, proinflammatory cytokines, apoptosis and toll like receptor 4 by empagliflozin to ameliorate bleomycin-induced lung fibrosisPubmed: 31600583
  • The protective effect of Lactobacillus versus 5-aminosalicylic acid in ulcerative colitis model by modulation of gut microbiota and Nrf2/Ho-1 pathwayPubmed: 32526285
  • Intraoperative dexmedetomidine for prevention of postoperative delirium in elderly patients with mild cognitive impairmentPubmed: 28593326
  • Isoliquiritigenin attenuates inflammation and modulates Nrf2/caspase-3 signalling in STZ-induced aortic injury33793806
  • In vitro protoscolicidal effects of lithocholic acid on protoscoleces of Echinococcus granulosus and its mechanismPubmed:35594934
  • Flavonoid 4, 4′-dimethoxychalcone induced ferroptosis in cancer cells by synergistically activating Keap1/Nrf2/HMOX1 pathway and inhibiting FECHPubmed:35697292

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