ELISA Kit DIY Materials for Heparan Sulfate Proteoglycan (HSPG) Homo sapiens (Human) Assay Kit DIY Materials

SDC2; HSPG1; SYND2; Fibroglycan; Syndecan 2; Syndecan Proteoglycan 2; Heparan Sulfate Proteoglycan 1,Cell Surface-Associated; Heparan Sulphate Proteoglycan

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Overview
Properties
  • Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable SampleSerum, plasma, tissue homogenates and other biological fluids.
  • Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
  • Detectable Range15.6-1000pg/mL
  • Applicable Sensitivity5.9pg/mL
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  • ELISA Kit DIY Materials for Heparan Sulfate Proteoglycan (HSPG) Packages (Simulation)
  • ELISA Kit DIY Materials for Heparan Sulfate Proteoglycan (HSPG) Packages (Simulation)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The Abs in the kit have high sensitivity and excellent specificity for detection of Heparan Sulfate Proteoglycan (HSPG). No significant cross-reactivity or interference between Heparan Sulfate Proteoglycan (HSPG) and analogues was observed.

Usage

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

Storage

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

Support pack

Citations

  • Cytotoxic effects of antiglypican-3 against HepG3 cell lineEbscohost: Source
  • Fibrinogen, an endogenous ligand of Toll-like receptor 4, activates monocytes in pre-eclamptic patientsPubmed: 24661950
  • Suramin inhibits hepatic tissue damage in hepatocellular carcinoma through deactivation of heparanase enzymePubmed: 24530413
  • Evaluation of antiglypican-3 therapy as a promising target for amelioration of hepatic tissue damage in hepatocellular carcinomaPubmed:25449037
  • Impairment of the Endothelial Glycocalyx in Cardiogenic Shock and its Prognostic RelevancePubmed:25692257
  • Endothelial glycocalyx layer shedding following lung resectionpubmed:27643669
  • Circulating syndecans during critical illness.pubmed:28256016
  • Cytotoxic and partial hepatoprotective activity of sodium ascorbate against hepatocellular carcinoma through inhibition of sulfatase-2 andPubmed:29669302
  • Glioma targeting peptide modified apoferritin nanocagePubmed:29726297
  • Postoperative microcirculatory perfusion and endothelial glycocalyx shedding following cardiac surgery with cardiopulmonary bypassPubmed: 30687934
  • Diet Supplementation with a Bioactive Pomace Extract from Olea europaea Partially Mitigates Negative Effects on Gut Health Arising from a Short-Term Fasting Period …
  • Heparin prevents in vitro glycocalyx shedding induced by plasma from COVID-19 patients33781826
  • A novel mutation in ext2 caused hereditary multiple exostoses through reducing the synthesis of heparan sulfate34042151
  • Response of broiler chickens fed diets supplemented with a bioactive olive pomace extract from Olea europaea to an experimental coccidial vaccine?¡­33518110
  • The effect of pre-operative methylprednisolone on postoperative delirium in elderly patients undergoing gastrointestinal surgery: a randomized, double-blind, placebo …34423832

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