ELISA Kit DIY Materials for High Mobility Group Protein 1 (HMGB1) Rattus norvegicus (Rat) Assay Kit DIY Materials

HMG1; HMG3; SBP1; Sulfoglucuronyl Carbohydrate Binding Protein; Amphoterin; High Mobility Group Box 1 Protein

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Overview
Properties
  • Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable SampleSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
  • Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
  • Detectable Range31.2-2,000pg/mL
  • Applicable Sensitivity12.5pg/mL
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  • ELISA Kit DIY Materials for High Mobility Group Protein 1 (HMGB1) Packages (Simulation)
  • ELISA Kit DIY Materials for High Mobility Group Protein 1 (HMGB1) Packages (Simulation)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The Abs in the kit have high sensitivity and excellent specificity for detection of High Mobility Group Protein 1 (HMG1). No significant cross-reactivity or interference between High Mobility Group Protein 1 (HMG1) and analogues was observed.

Usage

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

Storage

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

Support pack

Citations

  • High mobility group box protein-1 (HMGB-1) as a new diagnostic marker in patients with acute appendicitisPubMed: PMC3094252
  • Protective Effect of Nicotine on Lipopolysaccharide-Induced Acute Lung Injury in MiceKarger: 000319151
  • Clinical relevance of single nucleotide polymorphisms of the high mobility group box 1 protein gene in patients with major trauma in Southwest ChinaPubmed: 22047946
  • Is HMGB1 a New Indirect Marker for Revealing Fibrosis in Chronic Hepatitis and a New Therapeutic Target in Treatment?Wiley: source
  • Severity of sepsis is correlated with the elevation of serum high-mobility group box 1 in ratsCMJ: 2009218808935901080
  • Diagnostic value of serum concentrations of high-mobility group-box protein 1 and soluble hemoglobin scavenger receptor in brucellosisPubMed: 23278480
  • High mobility group B1 levels in sepsis and disseminated intravascular coagulationActabp: Source
  • The Inhibitory Effect of Lidocaine on the Release of High Mobility Group Box 1 in Lipopolysaccharide-Stimulated MacrophagesPubMed: 21288974
  • Elevated serum levels of high mobility group box protein 1 (HMGB1) in patients with ankylosing spondylitis and its association with disease activity and quality of lifePubMed: 23143556
  • Mammalian target of rapamycin complex 2 regulates inflammatory response to stressPubMed: 22899279
  • IGF-1 alleviates ox-LDL-induced inflammation via reducing HMGB1 release in HAECs PubMed: 22782142
  • The suppression of TRIM21 and the accumulation of IFN-α play crucial roles in the pathogenesis of osteonecrosis of the femoral head.PubMed: 22825687
  • Decreased Epidermal Growth Factor (EGF) Associated with HMGB1 and Increased Hyperactivity in Children with AutismPubMed: PMC3623607
  • ATF3 Protects against LPS-Induced Inflammation in Mice via Inhibiting HMGB1 ExpressionPubMed: PMC3770047
  • Correlation between serum high-mobility group box-1 levels and high-sensitivity C-reactive protein and troponin I in patients with coronary artery diseasePubmed: 23935732
  • Serum levels of high mobility group box 1 protein and its association with quality of life and psychological and functional status in patients with fibromyalgiaPubmed: 23901875
  • A novel serum marker for biliary tract cancer: Diagnostic and prognostic values of quantitative evaluation of serum mucin 5AC (MUC5AC)Pubmed: 24468034
  • Simvastatin suppresses vascular inflammation and atherosclerosis in ApoE-/- mice by downregulating the HMGB1-RAGE axisPubmed: 23564080
  • Soluble receptor for advanced glycation end-products and progression of airway diseasePubmed: 24758342
  • Ascorbic acid reduces HMGB1 secretion in lipopolysaccharide-activated RAW 264.7 cells and improves survival rate in septic mice by activation of Nrf2/HO-1 signalsPubmed:25896849
  • Effects of Single or Combined Treatments with Radiation and Chemotherapy on Survival and Danger Signals Expression in Glioblastoma Cell LinesHindawi:Source
  • High plasma levels of high mobility group box 1 is associated with the risk of sepsis in severe blunt chest trauma patients: a prospective cohort studyPubmed:Pmc4132233
  • HMGB1 is an early and critical mediator in an animal model of uveitis induced by IRBP-specific T cellsPubmed:24374967
  • The Alarmin Concept Applied to Human Renal Transplantation: Evidence for a Differential Implication of HMGB1 and IL-33Pubmed:24586382
  • Plasma levels of high mobility group box 1 increase in patients with posttraumatic stress disorder after severe blunt chest trauma: a prospective cohort studyPubmed:25016440
  • High-mobility group box-1, promising serological biomarker for the distinction of human WNV disease severityPubmed:25197038
  • Increased VEGF and Decreased SDF-1α in Patients with Silent Brain Infarction Are Associated with Better Prognosis after First-Ever Acute Lacunar Stroke Pubmed:25601176
  • 高迁移率族蛋白B1和神经元烯醇化酶对心脏骤停大鼠复苏后脑损伤评价的研究Journal: Fulltext
  • Diabetes-Induced Oxidative Stress in Endothelial Progenitor Cells May Be Sustained by a Positive Feedback Loop Involving High Mobility Group Box-1Journals: Omcl
  • Hesperidin ameliorates lipopolysaccharide-induced acute lung injury in mice by inhibiting HMGB1 releasePubMed: 25724384
  • Wogonin Inhibits Tumor-derived Regulatory Molecules by Suppressing STAT3 Signaling to Promote Tumor ImmunityPubMed: 25962106
  • Retinoic acid inhibits tissue factor and HMGB1 via modulation of AMPK activity in TNF-α activated endothelial cells and LPS-injected micePubMed: 26116962
  • Plasma HMGB-1 Levels in Subjects with Obesity and Type 2 Diabetes: A Cross-Sectional Study in ChinaPubMed: 26317615
  • Betulin attenuates lung and liver injuries in sepsisPubMed: 26644168
  • High mobility group box 1 gene polymorphism is associated with the risk of postoperative atrial fibrillation after coronary artery bypass surgeryPubMed: 26109393
  • Glycyrrhizin reduces HMGB1 secretion in lipopolysaccharide-activated RAW 264.7 cells and endotoxemic mice by p38/Nrf2-dependent induction of HO-1PubMed: 25812767
  • Overexpression of HMGB1 A-box reduced lipopolysaccharide-induced intestinal inflammation via HMGB1/TLR4 signaling in vitroPubMed: 26167076
  • Allopurinol reduces severity of delayed neurologic sequelae in experimental carbon monoxide toxicity in ratsPubMed: 25845300
  • HMGB1 expression patterns during the progression of experimental autoimmune encephalomyelitisPubMed: 25773152
  • HMGB1 release triggered by the interaction of live retinal cells and uveitogenic T cells is Fas/FasL activation-dependentPubMed: 26394985
  • Low Shear Stress Induced HMGB1 Translocation and Release via PECAM-1/PARP-1 Pathway to Induce Inflammation ResponsePubMed: 25793984
  • Interleukin-33 is released in spinal cord and suppresses experimental autoimmune encephalomyelitis in micePubMed: 26363151
  • Serum levels of HMGB1 have a diagnostic role in metastatic renal cell cancerPubmed:27062570
  • Oxymatrine attenuates CCl 4-induced hepatic fibrosis via modulation of TLR4-dependent inflammatory and TGF-β1 signaling pathwaysPubmed:27179304
  • Ethyl pyruvate attenuated coxsackievirus B3-induced acute viral myocarditis by suppression ofHMGB1/RAGE/NF-ΚB pathway.Pubmed:27026909
  • High mobility group box1 protein is involved in acute inflammation induced by Clostridium difficile toxin A.content:2016
  • COMPOSITIONS AND METHODS FOR INHIBITING HMGB1 ACTIVATION OF MELANOCYTESy2016:0077082.html
  • METHOD OF MAKING A SKIN CARE COMPOSITIONy2016:0077080.html
  • Oxidative Stress in Metabolic Disorders: Pathogenesis, Prevention, and TherapeuticsPubmed:26949450
  • Zoledronic acid-encapsulating self-assembling nanoparticles and doxorubicin: a combinatorial approach to overcome simultaneously chemoresistance and immunoresistance in breast tumorspubmed:26980746
  • Relevance of pre-analytical blood management on the emerging cardiovascular protein biomarkers TWEAK and HMGB1 and on miRNA serum and plasma profiling.pubmed:27847340
  • Serum HMGB1 as a Potential Biomarker for Patients with Asbestos-Related Diseases.pubmed:28348451
  • Changes in pro-inflammatory markers and leucine concentrations in response to Nordic Walking training combined with vitamin D supplementation in elderly women.pubmed:28316011
  • Serum HMGB1 serves as a novel laboratory indicator reflecting disease activity andpubmed:27800496
  • Ethyl pyruvate attenuated coxsackievirus B3-induced acute viral myocarditis by suppression of HMGB1/RAGE/NF-ΚB pathwaypubmed:27026909
  • HMGB1 blockade differentially impacts pulmonary inflammation and defense responses in poly (I: C)/LPS-exposed heart transplant micepubmed:27387278
  • Reduced HMGB 1-Mediated Pathway and Oxidative Stress in Resveratrol-Treated Diabetic Mice: A Possible Mechanism of Cardioprotection of Resveratrol in …pubmed:27833703
  • High Mobility Group Box1 Protein Is Involved in Endoplasmic Reticulum Stress Induced by Clostridium difficile Toxin Apubmed:27579314
  • The role of high mobility group box 1 protein in interleukin-18-induced myofibroblastic transition of valvular interstitial cellspubmed:27395056
  • HMGB1 exacerbates experimental mouse colitis by enhancing innate lymphoid cells 3 inflammatory responses via promoted IL-23 productionpubmed:27670944
  • Urinary MCP-1、HMGB1 increased in calcium nephrolithiasis patients and the influence ofhypercalciuria on the production of the two cytokines.pubmed:27393275
  • Diagnostic Value of High-Mobility Group Box 1 (HMGB1) Protein in Acute and Perforated Appendicitis.pubmed:27922766
  • High Mobility Group Box 1 Mediates Interferon-r-Induced Phenotypic Modulation of Vascular Smooth Muscle Cells.pubmed:27579780
  • Blood markers of fibrinolysis and endothelial activation in canine babesiosis.pubmed:28363279
  • Glycyrrhizin ameliorates experimental colitis through attenuating interleukin-17-producing T cell responses via regulating antigen-presenting cells.pubmed:28108937
  • SIRT1 inhibits releases of HMGB1 and HSP70 from human umbilical vein endothelial cells caused by IL-6 and the serum from a preeclampsia patient and protects the cells from death.pubmed:28122311
  • Bone marrow-derived mesenchymal stem cells (BMSCs) repair acute necrotized pancreatitis by secreting microRNA-9 to target the NF-κB1/p50 gene in rats.pubmed:28373667
  • The expression of HMGB1 and the receptor for advanced glycation end products (RAGE) in epithelial ovarian cancerpdf:ICST-4-232.pdf
  • Anti-inflammatory effect of cinnamaldehyde and linalool from the leaf essential oil of Cinnamomum osmophloeum Kanehira in endotoxin-induced …S1021-9498(17)30081-9
  • SIRT1 inhibits releases of HMGB1 and HSP70 from human umbilical vein endothelial cells caused by IL-6 and the serum from a preeclampsia patient and protects the cells from deathDOI:10.1016/j.biopha.2017.01.087
  • Induction of Immunogenic Cell Death in Lymphoma Cells by Wharton’s Jelly Mesenchymal Stem Cell Conditioned Mediumpubmed:28918474
  • Mineral particles stimulate innate immunity through neutrophil extracellular traps containing HMGB1pubmed:29192209
  • Oxidized low-density lipoprotein promotes osteoclast differentiation from CD68 positive mononuclear cells by regulating HMGB1 releasepubmed:29146189
  • HMGB1 promotes HLF-1 proliferation and ECM production through activating HIF1-a-regulated aerobic glycolysispubmed:28571757
  • Correlation between high mobility group box 1 protein, tumor necrosis factor-α, and acute coronary syndromeISSN 0970-938X
  • PERK induces resistance to cell death elicited by endoplasmic reticulum stress and chemotherapypubmed:28499449
  • Assessing the recovery from prerenal and renal acute kidney injury after treatment with single herbal medicine via activity of the biomarkers HMGB1, NGAL and KIM-1 in kidney proximal tubular cells treated by cisplatin with different doses and exposure times.pubmed:29258482
  • Blocking the Feedback Loop between Neuroendocrine Differentiation and Macrophages Improves the Therapeutic Effects of Enzalutamide (MDV3100) on Prostate Cancer.pubmed:29191973
  • Secreted tryptophanyl-tRNA synthetase as a primary defence system against infection.pubmed:27748732
  • Macrophage Inflammatory Protein-2 in High Mobility Group Box 1 Secretion of Macrophage Cells Exposed to Lipopolysaccharide.pubmed:28662496
  • Niclosamide attenuates inflammatory cytokines via the autophagy pathway leading to improved outcomes in renal ischemia/reperfusion injurypubmed:28627643
  • AMP-activated protein kinase agonist N 6-(3-hydroxyphenyl) adenosine protects against fulminant hepatitis by suppressing inflammation and apoptosisArticles:s41419-017-0118-0
  • S1 PR 3 is essential for phosphorylated fingolimod to protect astrocytes against oxygen‐glucose deprivation‐induced neuroinflammation via inhibiting TLR 2/4‐NF κB …Pubmed:29536648
  • Glycyrrhizin Protects Mice against Experimental Autoimmune Encephalomyelitis by Inhibiting HMGB1 Expression and Neuronal HMGB1 ReleasePubmed:30013568
  • Bromodomain inhibition exerts its therapeutic potential in malignant pleural mesothelioma by promoting immunogenic cell death and changing the tumor immune …Pubmed:29399399
  • Tumor lysate-based vaccines: on the road to immunotherapy for gallbladder cancerPubmed:29600445
  • Porcine reproductive and respiratory syndrome virus induces concurrent elevation of High Mobility Group Box-1 protein and pro-inflammatory cytokines in …Pubmed:29895396
  • Ursolic Acid Ameliorates Inflammation in Cerebral Ischemia and Reperfusion Injury Possibly via High Mobility Group Box 1/Toll-Like Receptor 4/NFκB …Pubmed:29867706
  • Oridonin enhances the cytotoxicity of 5-FU in renal carcinoma cells by inducting necroptotic deathPubmed:29958141
  • Luteolin activates ERK1/2- and Ca-dependent HO-1 induction that reduces LPS-induced HMGB1, iNOS/NO, and COX-2 expression in RAW264.7 cells and …Pubmed:29497773
  • Anti-inflammatory effect of cinnamaldehyde and linalool from the leaf essential oil of Cinnamomum osmophloeum Kanehira in endotoxin-induced micePubmed:29389558
  • Sirt1 S-nitrosylation induces acetylation of HMGB1 in LPS-activated RAW264. 7 cells and endotoxemic micePubmed:29680657
  • Thrombomodulin, alarmin signaling, and copeptin: cross-talk between obesity and acute ischemic stroke initiation and severity in EgyptiansPubmed:29637447
  • Increased serum levels of high mobility group protein B1 and calprotectin in pre‐eclampsiaPubmed:29569400
  • Molecules of Damage-Associated Patterns in Bronchoalveolar Lavage Fluid and Serum in Chronic Obstructive Pulmonary DiseasePubmed:29429028
  • HMGB1-RAGE signaling pathway in pPROMPubmed:29673663
  • Chronic obstructive pulmonary disease (COPD)--from biomarkers to clinical phenotypesISBN:978-951-51-4039-5
  • Butyrate suppresses abnormal proliferation in colonic epithelial cells under diabetic state by targeting HMGB1Doi: 10.1016/j.jphs.2018.07.012
  • Delivery of 5'-Triphosphate RNA with Endosomolytic Nanoparticles Potently Activates RIG-I to Improve Cancer ImmunotherapyDoi: 10.1039/C8BM01064A 
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  • Apoptosis resistance in fibroblasts precedes progressive scarring in pulmonary fibrosis and is partially mediated by Toll-like receptor 4 activationPubmed: 31020321
  • FBXW7 suppresses HMGB1-mediated innate immune signaling to attenuate hepatic inflammation and insulin resistance in a mouse model of nonalcoholic …Pubmed: 31215394
  • Dulaglutide mitigates inflammatory response in fibroblast-like synoviocytesPubmed: 31185450
  • Network pharmacology oriented study reveals inflammatory state-dependent dietary supplement hepatotoxicity responses in normal and diseased ratsPubmed: 31140472
  • BET bromodomain inhibitor JQ1 promotes immunogenic cell death in tongue squamous cell carcinomaPubmed: 31600692
  • High mobility group box protein 1 neutralization therapy in ovine bacteremia: Lessons learned from an ovine septic shock model incorporating intensive care supportPubmed: 31602200
  • Evaluation of the Effect Derived from Silybin with Vitamin D and Vitamin E Administration on Clinical, Metabolic, Endothelial Dysfunction, Oxidative Stress Parameters …Pubmed: 31737175
  • Magnetic hyperthermia using self-controlled heating elements consisting of Fe-Al milling alloy induces cancer cell apoptosis while preserving skeletal musclePubmed: 31722347
  • Suppression of high-mobility group box 1 ameliorates xerostomia in a sjogren syndrome-triggered mouse modelPubmed: 31935120
  • HMGB1 Mediates Paraquat-Induced Neuroinflammatory Responses via Activating RAGE Signaling PathwayPubmed: 31858421
  • Damage-Associated Molecular Patterns and Th-Cell-Related Cytokines Released after Progressive EffortPubmed: 32210109
  • Dendritic Cells Loaded with Heat Shock-Conditioned Ovarian Epithelial Carcinoma Cell Lysates Elicit T Cell-Dependent Antitumor Immune Responses In VitroPubmed: 31886313
  • A novel diagnostic tool for the detection of bladder cancer: Measurement of urinary high mobility group box-1Pubmed: 32312640
  • Marathon Run-induced Changes in the Erythropoietin-Erythroferrone-Hepcidin Axis are Iron DependentPubmed: 32316587
  • Upregulation of HMGB1 Secretion in Lungs of Pigs Infected by Highly Pathogenic Porcine Reproductive and Respiratory Syndrome VirusPubmed: 33221069
  • Extracellular cold-inducible RNA-binding protein regulates neutrophil extracellular trap formation and tissue damage in acute pancreatitisPubmed: 32709888
  • Inhibition of calpain alleviates coxsackievirus B3-induced myocarditis through suppressing the canonical NLRP3 inflammasome/caspase-1-mediated and …Pubmed: 32509190
  • A Novel Evidence That Mannan Binding Lectin (MBL) Pathway of Complement Cascade Activation is Involved in Homing and Engraftment of Hematopoietic Stem …Pubmed: 32406006
  • Glycyrrhizin Prevents Hemorrhagic Transformation and Improves Neurological Outcome in Ischemic Stroke with Delayed Thrombolysis Through Targeting …
  • Hypoxic Wharton's Jelly Stem Cell Conditioned Medium Induces Immunogenic Cell Death in Lymphoma Cells
  • Aberrant Expression of High Mobility Group Box Protein 1 in the Idiopathic Inflammatory Myopathies
  • Naja sumatrana venom cytotoxin, sumaCTX exhibits concentration-dependent cytotoxicity via caspase-activated mitochondrial-mediated apoptosis without …
  • Umbilical cord mesenchymal stem cells (hUCMSCs) for inflammatory regulation after excision and grafting of severe burn wounds in rats33313794
  • DAMPs released by pyroptotic cells as major contributors and therapeutic targets for CAR-T-related toxicities33504767
  • Insights into P-Glycoprotein Inhibitors: New Inducers of Immunogenic Cell Death32331368
  • A non-lethal murine flame burn model leads to a transient reduction in host defenses and enhanced susceptibility to lethal Pseudomonas aeruginosa infection34152806
  • Inhaled [D-Ala2]-Dynorphin 1-6 Prevents Hyperacetylation and Release of High Mobility Group Box 1 in a Mouse Model of Acute Lung Injury34616852
  • Autophagy-associated HMGB-1 as a novel potential circulating non-invasive diagnostic marker for detection of Urothelial Carcinoma of Bladder34796446
  • Calycosin attenuates severe acute pancreatitis-associated acute lung injury by curtailing high mobility group box 1-induced inflammation34908806
  • Clinical significance of high mobility group box 1/toll-like receptor 4 in obese diabetic patients34657898
  • Choline or CDP-choline restores hypotension and improves myocardial and respiratory functions in dogs with experimentally–Induced endotoxic shock34715589
  • Intracellular translocation of HMGB1 is important for Zika virus replication in Huh7 cellsPubmed:35058496
  • High Mobility Group Box-1 Protein and Interleukin 33 Expression in Allergic RhinitisPubmed:34979505
  • SKP2 drives the sensitivity to neddylation inhibitors and cisplatin in malignant pleural mesotheliomaPubmed:35197103
  • Abnormal intestinal milieu in post-traumatic stress disorder is not impacted by treatment that improves symptomsPubmed:35638693
  • Circulating markers of endothelial activation in canine parvoviral enteritis
  • The antiviral action of resveratrol against Zika virus through the regulation of interferon responses by HMGB1

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