ELISA Kit DIY Materials for Histamine (HA)

Histamin

UOM
1. 96T*5
2. 96T*10
3. 96T*20
4. 96T*50
5. 96T*100
FOB US$ 1,433.00 US$ 2,388.00 US$ 4,298.00 US$ 8,358.00 US$ 14,328.00
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Overview

Properties

Product No.KSA927Ge11
Organism SpeciesPan-species (General) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Canine
- Porcine
- Bovine
- Caprine
- Multi-species
- Pan-species
- Others
ApplicationsMain materials for "Do It(ELISA Kit) Yourself"
Research use only
Downloadn/a
CategorySignal transduction Infection immunity

Reagent Contents Capture Antibody, Biotin-labeled Competitor, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
Detectable SampleSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Applicable PrincipleCompetitive Inhibition ELISA for Antigen Detection
Detectable Range1.23-100ng/mL
Applicable Sensitivity0.52ng/mL

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Packages (Simulation)
Packages (Simulation)

ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The Abs in the kit have high sensitivity and excellent specificity for detection of Histamine (HA). No significant cross-reactivity or interference between Histamine (HA) and analogues was observed.

Usage

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

Storage

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

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ELISA / CLIA Experiment Service

Increment services

Citations

PEST-domain-enriched tyrosine phosphatase and glucocorticoids as regulators of anaphylaxis in micePubmed: 21981059
Comparison of sensitization between β-lactoglobulin and its hydrolysatesDigital: 875
Integrative analysis of proteomics and metabolomics of anaphylactoid reaction induced by Xuesaitong injectionPubMed: 26372445
Expression of inducible nitric oxide synthase in mast cells contributes to the reCavia (Guinea pig )lation of inflammatory cytokines in irritable bowel syndrome with diarrheaPubmed:26940641
Induction of immune responses and allergic reactions in piglets by injecting glycinindoi:1828051X.2016.1144488
Effects of Dectin-1 on the mast cells in allergic conjunctivitis and its underlying mechanismfiles:ijcep0019305.pdf
Proteomics Study on Nonallergic Hypersensitivity Induced by Compound 4880 and Ovalbuminpmc:PMC4734762
Evaluation of anaphylactoid constituents in vitro and in vivo.pubmed:27984711
Ultraviolet A photosensitivity profile of dexchlorpheniramine maleate and promethazine-based creams: Anti-inflammatory,antihistaminic, and skin barrier protection propertiesDOI: 10.1111/jocd.12349
Adipose Tissue-Derived Mesenchymal Stem Cell Modulates the Immune Response of Allergic Rhinitis in a Rat ModelPubmed: 30781605
Metabolomics analysis of baicalin on ovalbumin-sensitized allergic rhinitis rats
MOTION SICKNESS INDUCES PHYSIOLOGICAL AND NEURONAL ALTERATIONS IN MOUSE MODEL
Artemisia annua sublingual immunotherapy for seasonal allergic rhinitis: a randomized controlled trialPubmed: 32030780
Animal models for analysis of hypersensitivity reactions to Shuanghuanglian injectionPubmed: 32302806
Protective effect of Asarum sieboldii essential oil on ovalbumin induced allergic rhinitis in ratPubmed: 32395767
A vaccine-based nanosystem for initiating innate immunity and improving tumor immunotherapyPubmed: 32332752
CCR3‑shRNA promotes apoptosis and inhibits chemotaxis and degranulation of mouse mast cellsPubmed: 32742345
Multiplexed fluorescence immunoassay combined with magnetic separation using upconversion nanoparticles as multicolor labels for the simultaneous detection of …Pubmed: 32892931
Study of some hematological and immunological parameters associated with the infection of intestinal parasites in the holy city of Kerbala, Iraq
Pharmacokinetics and safety of TCMCB07, a melanocortin-4 antagonist peptide in dogs34014033