ELISA Kit DIY Materials for Hyaluronic Acid (HA) Pan-species (General) Assay Kit DIY Materials

Hyaluronan; Hyaluronate

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Overview
Properties
  • Reagent Contents Capture Antibody, Biotin-labeled Competitor, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable SampleSerum, plasma and other biological fluids
  • Applicable PrincipleCompetitive Inhibition ELISA for Antigen Detection
  • Detectable Range4.94-400ng/mL
  • Applicable Sensitivity
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  • ELISA Kit DIY Materials for Hyaluronic Acid (HA) Packages (Simulation)
  • ELISA Kit DIY Materials for Hyaluronic Acid (HA) Packages (Simulation)
  • KSA182Ge11.jpg Picture
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The Abs in the kit have high sensitivity and excellent specificity for detection of Hyaluronic Acid (HA). No significant cross-reactivity or interference between Hyaluronic Acid (HA) and analogues was observed.

Usage

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

Storage

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

Support pack

Citations

  • Hemorrhagic Fever Caused by a Novel Bunyavirus in China: Pathogenesis and Correlates of Fatal OutcomeOxfordJournals: source
  • Alveolar fluid in acute respiratory distress syndrome promotes fibroblast migration: Role of platelet-derived growth factor pathway*PubMed: 22713216
  • Age effects on extracellular matrix production of vocal fold scar fibroblasts in ratsPubmed: 24077847
  • Vocal Fold Fibroblast Response to Growth Factor Treatment is Age Dependent: Results From an In Vitro StudyScienceDirect: S0892199713002415
  • Resveratrol inhibits ovarian cancer cell adhesion to peritoneal mesothelium in vitro by modulating the production of α5β1 integrins and hyaluronic acidPubmed:24995580
  • Establishing principles of macromolecular crowding for in vitro fibrosis research of the vocal fold lamina propriaPubmed:25545625
  • Pituitary tumor transforming gene as a novel regulatory factor of liver fibrosisPubMed: 25936962
  • Camel Milk: Potential Utility as an Adjunctive Therapy to Peg-IFN/RBV in HCV-5 Infected Patients in EgyptPubMed: 26492131
  • Study of Some Serum Biochemical Markers of Liver Fibrosis in Patients with Chronic HCVISSN 2575-7733
  • A putative Chondroprotective role for IL-1β and MPO in herbal treatment of experimental osteoarthritispubmed:29166937
  • Polycomb group proteins: Novel molecules associated with ultraviolet A-induced photoaging of human skin.pubmed:28962194
  • The effect of aerobic walking and lower body resistance exercise on serum COMP and hyaluronan, in both males and femalesPubmed:29536174
  • Evaluation of antifibrotic effects of coffee and cocoa extracts in rats with thioacetamide-induced fibrosis10.1007:s00217-018-3119-z
  • Juvenile Ovine Ex Vivo Larynges: Phonatory, Histologic, and Micro CT Based Anatomic Analyses
  • Selected elements of extracellular matrix of the skin in diabetes and insulin resistancePubmed: 31146169
  • Strain-Dependent Porcine Circovirus Type 2 (PCV2) Entry and Replication in T-LymphoblastsPubmed: 31480752
  • Betulin attenuated liver damage by prevention of hepatic mitochondrial dysfunction in rats with alcoholic steatohepatitisPubmed: 31619044
  • Damage-Associated Molecular Patterns and Th-Cell-Related Cytokines Released after Progressive EffortPubmed: 32210109
  • iTRAQ-based analysis for the identification of MARCH8 targets in human esophageal squamous cell carcinoma33540066
  • An exploratory study to investigate the association between age, physical activity, femoral trochlear cartilage thickness and biomarkers of tissue metabolism in?¡­33713200
  • Trimethylamine N‐oxide exacerbates acetaminophen‐induced liver injury by interfering with macrophage‐mediated liver regeneration34459512
  • Comparative studies on the effect of pig adipose-derived stem cells (pASCs) preconditioned with hypoxia or normoxia on skin wound healing in micePubmed:35718003
  • Bicyclol ameliorates advanced liver diseases in murine models via inhibiting the IL-6/STAT3 signaling pathwayPubmed:35658240
  • Novel multifunctional delivery system for chondrocytes and articular cartilage based on carbon quantum dots

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