ELISA Kit DIY Materials for Indole 3 Acetic Acid (IAA)
IA-A; Indolylacetic Acid; Indoleacetic Acid; Heteroauxin
- UOM
- FOB US$ 1,433.00 US$ 2,388.00 US$ 4,298.00 US$ 8,358.00 US$ 14,328.00
- Quantity
Overview
Properties
- Product No.KSA737Ge11
- Organism SpeciesPan-species (General) Same name, Different species.
- ApplicationsMain materials for "Do It (ELISA Kit) Yourself".
Research use only - DownloadInstruction Manual
- CategorySignal transductionHormone metabolism
- Reagent Contents Capture Antibody, Biotin-labeled Competitor, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
- Detectable Sampletissue or cell culture supernates
- Applicable PrincipleCompetitive Inhibition ELISA for Antigen Detection
- Detectable Range640-400,000pg/mL
- Applicable Sensitivity227pg/mL
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Specifity
The Abs in the kit have high sensitivity and excellent specificity for detection of Indole 3 Acetic Acid (IAA). No significant cross-reactivity or interference between Indole 3 Acetic Acid (IAA) and analogues was observed.
Usage
1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.
Storage
Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.
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Citations
- Assisted phytoremediation of mixed metal(loid)-polluted pyrite waste: effects of foliar and substrate IBA application on fodder radishPubMed: 21570105
- Contribution of indole-3-acetic acid in the plant growth promotion by the rhizospheric strain Bacillus amyloliquefaciens SQR9Springer:Source
- Structural and metabolic alterations in root systems under limited water conditions in forage grasses of Lolium-Festuca complex
- Indole-3-Acetic Acid Alleviates Nonalcoholic Fatty Liver Disease in Mice via Attenuation of Hepatic Lipogenesis, and Oxidative and Inflammatory StressPubmed: 31484323