ELISA Kit DIY Materials for Indoleamine-2,3-Dioxygenase (IDO) Homo sapiens (Human) Assay Kit DIY Materials

CD107B; INDO; Indoleamine-Pyrrole 2,3 Dioxygenase

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Overview
Properties
  • Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable SampleSerum, plasma, tissue homogenates, cell lysates and other biological fluids.
  • Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
  • Detectable Range0.312-20ng/mL
  • Applicable Sensitivity0.131ng/mL
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  • ELISA Kit DIY Materials for Indoleamine-2,3-Dioxygenase (IDO) Packages (Simulation)
  • ELISA Kit DIY Materials for Indoleamine-2,3-Dioxygenase (IDO) Packages (Simulation)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The Abs in the kit have high sensitivity and excellent specificity for detection of Indoleamine-2,3-Dioxygenase (IDO). No significant cross-reactivity or interference between Indoleamine-2,3-Dioxygenase (IDO) and analogues was observed.

Usage

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

Storage

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

Support pack

Citations

  • CCL18 differentiates dendritic cells in tolerogenic cells able to prime regulatory T cells in healthy subjectsPubmed: 21803856
  • Potential immunosuppressive function of plasma indoleamine 2,3-dioxygenase in patients with aGVHD after allo-HSCTWiley: source
  • Secretion of Indoleamine 2, 3-Dioxygenase, an Immunomodulatory Substance, by Adipose-Derived Mesenchymal Stem CellIregway: Source
  • CD40 Gene Silencing Reduces the Progression of Experimental Lupus Nephritis Modulating Local Milieu and Systemic MechanismsPubMed: PMC3683035
  • Human CD14+ CTLA-4+ regulatory dendritic cells suppress T-cell response by cytotoxic T-lymphocyte antigen-4-dependent IL-10 and indoleamine-2,3-dioxygenase production in hepatocellular carcinomaPubmed: 23960017
  • Indoleamine-2,3-dioxygenase elevated in tumor-initiating cells is suppressed by mitocansScienceDirect: S0891584913006345
  • Cancer-Associated Fibroblasts from lung tumors maintain their immuno-suppressive abilities after high-dose irradiationFrontiersin:Source
  • Immunogenicity and escape mechanisms of allogeneic tendon-derived stem cellsPubmed:24813640
  • The expression of dendritic cell subsets in severe chronic rhinosinusitis with nasal polyps is alteredPubmed:24947894
  • Chronic hepatitis C virus infection triggers spontaneous differential expression of biosignatures associated with T cell exhaustion and apoptosis signaling in peripheral blood mononucleocytesPubmed:25577277
  • Cancer-Associated Fibroblasts from Lung Tumors Maintain Their Immunosuppressive Abilities after High-Dose IrradiationPubMed: 26029659
  • Insights into the use of adipose stem cells for clinical cell therapy: Novel culturing conditions and characterization of multipotency and immunogenic propertiesHandle: 10024
  • Respiratory Syncytial Virus-Infected Mesenchymal Stem Cells Regulate Immunity via Interferon Beta and Indoleamine-2, 3-Dioxygenasepubmed:27695127
  • Exploring Amino Acid-Capped Nanoparticles for Selective Anti-Parasitic Action and Improved Host BiocompatibilityPubmed:29883557
  • Mechanisms of Leptin and Ghrelin Action on Maturation and Functions of Dendritic Cells
  • Cognitive Decline, Cerebral-Spleen Tryptophan Metabolism, Oxidative Stress, Cytokine Production, and Regulation of the Txnip Gene in 3xTg-AD MicePubmed: 30980800
  • Interaction of the immune-inflammatory and the kynurenine pathways in rats resistant to antidepressant treatment in model of depressionPubmed: 31176083
  • Richard Schäfer,* Gabriele Spohn, Marco Bechtel, 2 Denisa Bojkova, 2 Patrick C. Baer, 3 Selim Kuçi, 4
  • Altered Indoleamine 2, 3-Dioxygenase Production and Its Association to Inflammatory Cytokines in Peripheral Blood Mononuclear Cells Culture of Type 2 Diabetes?¡­33343199
  • Richard Sch?fer,* Gabriele Spohn, Marco Bechtel, 2 Denisa Bojkova, 2 Patrick C. Baer, 3 Selim Ku?i, 4
  • Associations between expression of indoleamine 2, 3-dioxygenase enzyme and inflammatory cytokines in patients with first-episode drug-naive …34802039

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