ELISA Kit DIY Materials for Insulin Like Growth Factor 2 (IGF2)
INSIGF; IGF-II; Somatomedin A; Preptin; Insulin-like growth factor II Ala-25 Del
- UOM
- FOB US$ 1,231.00 US$ 2,052.00 US$ 3,694.00 US$ 7,182.00 US$ 12,312.00
- Quantity
Overview
Properties
- Product No.KSA051Ra01
- Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
- ApplicationsMain materials for "Do It(ELISA Kit) Yourself"
Research use only - Downloadn/a
- CategoryTumor immunityEndocrinology
- Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
- Detectable SampleSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
- Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
- Detectable Range78.12-5000pg/mL
- Applicable Sensitivity30pg/mL
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Specifity
The Abs in the kit have high sensitivity and excellent specificity for detection of Insulin Like Growth Factor 2 (IGF2). No significant cross-reactivity or interference between Insulin Like Growth Factor 2 (IGF2) and analogues was observed.
Usage
1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.
Storage
Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.
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Citations
- EVALUATION OF PHYSICO-CHEMICAL PROPERTIES OF COLOSTRUM SUPPLEMENTED DAHIIjfans: Source
- Studies on quality attributes of skimmed colostrum powderP-ISSN: 2349–8528
- Increased motor neuron resilience by small molecule compounds that regulate IGF-II expressionPubmed:29113829
- IGFBP2 Plays an Essential Role in Cognitive Development during Early LifePubmed: 31832311
- Altered insulin-like growth factor-2 signaling is associated with psychopathology and cognitive deficits in patients with schizophreniaPubmed: 32191705
- Changes of Serum Insulin-like Growth Factor-2 Response to Negative Symptom Improvements in Schizophrenia Patients Treated with Atypical AntipsychoticsPubmed: 32681260
- Gastrodin Exerts Cardioprotective Action via Inhibition of Insulin-Like Growth Factor Type 2/Insulin-Like Growth Factor Type 2 Receptor Expression in Cardiac …34250336