ELISA Kit DIY Materials for Insulin Receptor (INSR) Homo sapiens (Human) Assay Kit DIY Materials

CD220; ISR; HHF5; IR

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Overview
Properties
  • Reagent Contents Capture Antibody, Biotin-labeled Competitor, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable SampleSerum, plasma, tissue homogenates, cell culture supernates and other biological fluids.
  • Applicable PrincipleCompetitive Inhibition ELISA for Antigen Detection
  • Detectable Range2.47-200ng/mL
  • Applicable Sensitivity0.92ng/mL
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  • ELISA Kit DIY Materials for Insulin Receptor (INSR) Packages (Simulation)
  • ELISA Kit DIY Materials for Insulin Receptor (INSR) Packages (Simulation)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The Abs in the kit have high sensitivity and excellent specificity for detection of Insulin Receptor (ISR). No significant cross-reactivity or interference between Insulin Receptor (ISR) and analogues was observed.

Usage

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

Storage

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

Support pack

Citations

  • Molecular and Biochemical Effect of Neem Extract On Experimental Diabetes Org:Source
  • Age-Dependent Onset of Insulin Resistance in Insulin-Resistant MicePubMed: 26632184
  • A newly developed silymarin nanoformulation as a potential antidiabetic agent in experimental diabetespubmed:27623396
  • Subcutaneous liraglutide ameliorates methylglyoxal-induced Alzheimer-like tau pathology and cognitive impairment by modulating tau hyperphosphorylation and glycogen synthase kinase-3β.pubmed:28337257
  • Vitamin D3 intake as regulator of insulin degrading enzyme and insulin receptor phosphorylationin diabetic rats.pubmed:27930980
  • Regulation of insulin receptor phosphorylation in the brains of prenatally stressed rats: New insight into the benefits of antidepressant drug treatment.pubmed:28063625
  • (台灣農業研究67 (4): 355-364) 香菇柄水萃物改善糖尿病降低阿茲海默氏症罹病風險初探
  • Mechanism underlying starvation-dependent modulation of olfactory behavior in Drosophila larvaPubmed: 32080342
  • Prenatal Stress Induced Spatial Memory Deficit in a Sex-Specific Manner in Mice: Possible Involvement of Hippocampal Insulin Resistance
  • Internal State Affects Local Neuron Function in an Early Sensory Processing Center to Shape Olfactory Behavior in Drosophila Larvae

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