ELISA Kit DIY Materials for Interferon Beta (IFNb) Chicken (Gallus) Assay Kit DIY Materials

IFNB1; IFN-B; IFB; IFF; IFNB; Interferon Beta 1 Fibroblast

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Overview
Properties
  • Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable SampleSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
  • Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
  • Detectable Range3.12-200pg/mL
  • Applicable Sensitivity1.22pg/mL
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  • ELISA Kit DIY Materials for Interferon Beta (IFNb) Packages (Simulation)
  • ELISA Kit DIY Materials for Interferon Beta (IFNb) Packages (Simulation)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The Abs in the kit have high sensitivity and excellent specificity for detection of Interferon Beta (IFNb). No significant cross-reactivity or interference between Interferon Beta (IFNb) and analogues was observed.

Usage

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

Storage

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

Support pack

Citations

  • Melatonin protects liver against ischemia and reperfusion injury through inhibition of toll-like receptor signaling pathwayPubMed: 21355876
  • Hepatitis B virus polymerase inhibits RIG-I- and Toll-like receptor 3-mediated beta interferon induction in human hepatocytes through interference with interferon regulatory factor 3 activation and dampening of the interaction between TBK1/IKKε and DDX3.Sgm: 9182080
  • Impaired TLR3/IFN-β signaling in monocyte-derived dendritic cells from patients with acute-on-chronic hepatitis B liver failure: Relevance to the severity of liver damageScienceDirect: S0006291X09019822
  • Intact Type I Interferon Production and IRF7 Function in Sooty MangabeysPubMed: PMC3757038
  • Innate Immune Response to Rift Valley Fever Virus in GoatsPubMed: PMC3335883
  • Low pH Environmental Stress Inhibits LPS and LTA-Stimulated Proinflammatory Cytokine Production in Rat Alveolar MacrophagesPubmed: 24288685
  • An attenuated EIAV strain and its molecular clone strain differentially induce the expression of Toll-like receptors and type-I interferons in equine monocyte-derived macrophages.Pubmed: 23850441
  • Scoparone attenuates d-galactosamine/lipopolysaccharide-induced fulminant hepatic failure through inhibition of toll-like receptor 4 signaling in micePubmed: 23535186
  • Experimental model of equine alveolar macrophage stimulation with TLR ligandsPubmed: 23815824
  • Training Modifies Innate Immune Responses in Blood Monocytes and in Pulmonary Alveolar MacrophagesPubmed: 24502337
  • Mannan binding lectin attenuates double-stranded RNA-mediated TLR3 activation and innate immunityPubmed:24530528
  • Infection of equine monocyte-derived macrophages with an attenuated equine infectious anemia virus (EIAV) strain induces a strong resistance to the infection by a virulent EIAV strainPubmed:25106750
  • MicroRNA-30e* Suppresses Dengue Virus Replication by Promoting NF-κB–Dependent IFN ProductionPubmed:25122182
  • The innate immune response of equine bronchial epithelial cells is altered by trainingPubmed:25595212
  • SOCS3 Drives Proteasomal Degradation of TBK1 and Negatively Regulates Antiviral Innate ImmunityPubMed: 25939384
  • Analysis of cytokine production in a newly developed canine tracheal epithelial cell line infected with H3N3 canine influenza virusPubMed: 25797196
  • Complexes of DNA with the Antimicrobial Peptide LL37 Augment NK Cell Functions by Inducing Type I Interferon Production from Circulating Monocytes and Plasmacytoid Predendritic CellsPubMed: 26258404
  • PKR activation enhances replication of classical swine fever virus in PK-15 cellsPubMed: 25899421
  • siRNA Targeting the 2A pro Genomic Region Prevents Enterovirus 71 Replication In VitroPubmed:26886455
  • Cellular uptake of exogenous calcineurin B is dependent on TLR4/MD2/CD14 complexes, and CnB is an endogenous ligand of TLR4Pubmed:27090571
  • Innate Immune Responses in ALV-J Infected Chicks and Chickens with Hemangioma In VivoPubmed:27252695
  • Chloroquine attenuates lipopolysaccharide-induced inflammatory responses through upregulation of USP25.pubmed:28134560
  • Effects of IRF1 and IFN-β interaction on the M1 polarization of macrophages and its antitumor functionpubmed:27176664
  • Host miR-146a promotes replication of human cytomegalovirus by suppressing type I IFN response in MRC-5 cellsfiles:ijcep0036479.pdf
  • ALV-J strain SCAU-HN06 induces innate immune responses in chicken primary monocyte-derived macrophagespubmed:27486255
  • Characterization of Simian Immunodeficiency Virus Variants Anatomically Compartmentalized inPlasma and Milk in Chronically Infected African Green Monkeys.pubmed:
  • MicroRNA-548j inhibits type I interferon production by targeting ZBTB11 in patients with chronic hepatitis Bpubmed:28342861
  • The down-regulation of casein kinase 1 alpha as a host defense response against infectious bursal disease virus infectionpubmed:28988058
  • Zika virus elicits inflammation to evade antiviral response by cleaving cGAS via NS1‐caspase‐1 axisPubmed:30065070
  • Bisphenol A induced male germ cell apoptosis via IFNβ-XAF1-XIAP pathway in adult micePubmed:30017639
  • MAVS induces a host cell defense to inhibit CSFV infectionPubmed:29556776
  • Inhibition of DNA-sensing pathway by Marek's disease virus VP23 protein through suppression of interferon regulatory factor 7 activationPubmed: 30518647
  • Molecular cloning and functional characterization of porcine 2′, 5′-oligoadenylate synthetase 1b and its effect on infection with porcine reproductive and respiratory?…Doi: 10.1016/j.vetimm.2019.01.003
  • HIPK2 is necessary for type I interferon–mediated antiviral immunityDoi: 10.1126/scisignal.aau4604
  • Evaluation of serum interferons in patients with age-related macular degenerationPubmed: 31007694
  • Differential expression of type I interferon mRNA and protein levels induced by virulent Marek's disease virus infection in chickens
  • Porcine FcγRIIb mediated PRRSV ADE infection through inhibiting IFN-β by cytoplasmic inhibitory signal transductionPubmed: 31284005
  • Marek's Disease Virus RLORF4 Inhibits Type I Interferon Production by Antagonizing NF-κB ActivationPubmed: 31243133
  • Serodiagnosis of Human Bocavirus 1 Infection among Hospitalised Children with Lower Respiratory Tract Infection in Latvia
  • The immunostimulatory effects and pro‐apoptotic activity of rhCNB against Lewis lung cancer is mediated by Toll‐like receptor 4Pubmed: 31218844
  • Scavenger receptor A impairs interferon response to HBV infection by limiting TRAF 3 ubiquitination through recruiting OTUB 1
  • Avian oncogenic herpesvirus antagonizes the cGAS-STING DNA-sensing pathway to mediate immune evasionPubmed: 31539404
  • Induction of autophagy and suppression of type I IFN secretion by CSFVPubmed: 32160078
  • BVDV-1 induces interferon-beta gene expression through a pathway involving IRF1, IRF7, and NF-κB activationPubmed: 33053462
  • LDHB inhibition induces mitophagy and facilitates the progression of CSFV infectionPubmed: 32924761
  • Infectious bronchitis virus inhibits activation of the TLR7 pathway, but not the TLR3 pathwayPubmed: 32532134
  • Determination of antiviral action of Long Non-coding RNA loc107051710 During Infectious Bursal Disease Virus Infection due to Enhancement of Interferon …
  • Transferrin-dependent crosstalk between the intestinal tract and commensal microbes contributes for immune tolerance
  • Open reading frame 5 protein of porcine circovirus type 2 induces RNF128 (GRAIL) which inhibits mRNA transcription of interferon-β in porcine epithelial cells34416463
  • Anti‐IFN‐α/‐ω neutralizing antibodies from COVID‐19 patients correlate with downregulation of IFN response and laboratory biomarkers of disease severityPubmed:35419822
  • Chicken Intestinal Microbiota Modulation of Resistance to Nephropathogenic Infectious Bronchitis Virus Infection Through IFN-I

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