ELISA Kit DIY Materials for Interleukin 10 (IL10) Equus caballus; Equine (Horse) Assay Kit DIY Materials

CSIF; IL10A; TGIF; Cytokine Synthesis Inhibitory Factor

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  • Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable SampleSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
  • Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
  • Detectable Range7.8-500pg/mL
  • Applicable Sensitivity2.6pg/mL
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  • ELISA Kit DIY Materials for Interleukin 10 (IL10) Packages (Simulation)
  • ELISA Kit DIY Materials for Interleukin 10 (IL10) Packages (Simulation)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered


The Abs in the kit have high sensitivity and excellent specificity for detection of Interleukin 10 (IL10). No significant cross-reactivity or interference between Interleukin 10 (IL10) and analogues was observed.


1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.


Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

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