ELISA Kit DIY Materials for Interleukin 33 (IL33) Homo sapiens (Human) Assay Kit DIY Materials

DV27; C9ORF26; IL1F11; NFHEV; Interleukin-1 Family, Member 11; Nuclear factor from high endothelial venules

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Overview
Properties
  • Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable SampleSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
  • Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
  • Detectable Range15.6-1,000pg/mL
  • Applicable Sensitivity6.4pg/mL
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  • ELISA Kit DIY Materials for Interleukin 33 (IL33) Packages (Simulation)
  • ELISA Kit DIY Materials for Interleukin 33 (IL33) Packages (Simulation)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The Abs in the kit have high sensitivity and excellent specificity for detection of Interleukin 33 (IL33). No significant cross-reactivity or interference between Interleukin 33 (IL33) and analogues was observed.

Usage

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

Storage

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

Support pack

Citations

  • Interleukin-33, matrix metalloproteinase-9, and tissue ınhibitor of matrix metalloproteinase-1 in myocardial infarctionPubMed: PMC3604606
  • CXCL13 blockade attenuates lupus nephritis of MRL/lpr micePubMed: 26456520
  • Epithelial Cell-Derived Cytokines Contribute to the Pathophysiology of Eosinophilic Chronic RhinosinusitisPubmed:26540312
  • The Expression and Regulation of Interleukin-33 in Human Epidermal Keratinocytes: A New Mediator of Atopic Dermatitis and Its Possible Signaling Pathway.pubmed:27348082
  • IL-33 circulating serum levels are increased in patients with non-segmental generalized vitiligopubmed:27388717
  • Chrysin Protects Rat Kidney from Paracetamol-Induced Oxidative Stress, Inflammation, Apoptosis, and Autophagy: A Multi-Biomarker Approach. pubmed:28134775
  • Association of IL-33, IL1RL1 gene polymorphisms with serum IL-33 levels and risk of asthma in adults and asthmatic bronchitis in children (Chinese)10.1080:13102818.2018.1471361
  • Role of IL-33 and ST2 signaling and inflammatory responses in non-small cell lung cancer172341
  • Group II innate lymphoid cells and microvascular dysfunction from pulmonary titanium dioxide nanoparticle exposurePubmed: 30413212
  • Interleukin-33 serum levels in postmenopausal women with osteoporosisPubmed: 30846811
  • Tumor-Derived Lactic Acid Contributes to the Paucity of Intratumoral ILC2sPubmed: 32101749
  • Relationship of microbial profile with airway immune response in eosinophilic or neutrophilic inflammation of asthmaticsPubmed: 32141256
  • Qingfei oral liquid downregulates TRPV1 expression to reduce airway inflammation and mucus hypersecretion injury caused by respiratory syncytial virus infection …
  • Aggravation of Airway Inflammation in RSV-Infected Asthmatic Mice Following Infection-Induced Alteration of Gut Microbiota
  • Inhibition of NF-κB/IL-33/ST2 Axis Ameliorates Acute Bronchiolitis Induced by Respiratory Syncytial Virus34395633
  • Elevated Levels of IL-33, IL-17 and IL-25 Indicate the Progression from Chronicity to Hepatocellular Carcinoma in Hepatitis C Virus PatientsPubmed:35056005
  • Dexmedetomidine Attenuates Hyperalgesia Induced By Brachial Plexus Root Avulsion By Restoring The GLT-1 Function Via PKA Signaling

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