ELISA Kit DIY Materials for Interleukin 35 (IL35) Homo sapiens (Human) Assay Kit DIY Materials

Add to Cart Distributors
Overview
Properties
  • Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable Sampleserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
  • Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
  • Detectable Range1.56-100pg/mL
  • Applicable Sensitivity
Share your citation Upload your experimental result Review Leave a message
Loading...

Share a new citation as an author

Upload your experimental result

Review

Please attach serial No. on instruction manual

Contact us

Please fill in the blank.

Name*
Organization
Address
E-mail address*
Telephone
Inquiry*
Verification code* CheckCode
  • ELISA Kit DIY Materials for Interleukin 35 (IL35) Packages (Simulation)
  • ELISA Kit DIY Materials for Interleukin 35 (IL35) Packages (Simulation)
  • KSC008Hu01.jpg Picture
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The Abs in the kit have high sensitivity and excellent specificity for detection of Interleukin 35 (IL35). No significant cross-reactivity or interference between Interleukin 35 (IL35) and analogues was observed.

Usage

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

Storage

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

Support pack

Citations

  • Aberrant expression of Treg-associated cytokine IL-35 along with IL-10 and TGF-β in acute myeloid leukemia.PubMed: 22783403
  • Plasma IL-17, IL-35, interferon-γ, SOCS3 and TGF-β levels in pregnant women with preeclampsia, and their relation with severity of diseasePubmed: 24175856
  • What is the impact of Th1/Th2 ratio, SOCS3, IL17, and IL35 levels in unexplained infertility?Pubmed: 24368037
  • The imbalanced profile and clinical significance of T helper associated cytokines in bone marrow microenvironment of the patients with acute myeloid leukemiaScienceDirect: S0198885913005776
  • Gene Expression of Subunits of the IL-12 Family Cytokines in moDCs Derived In Vitro from the Cord Blood of Children of Healthy and Allergic MothersPubmed:24785110
  • Function of interleukin?17 and ?35 in the blood of patients with hepatitis B?related liver cirrhosisPubmed:25323532
  • The regular distribution and expression pattern of immunosuppressive cytokine IL-35 in mouse uterus during early pregnancyPubmed:25611266
  • Wogonin Inhibits Tumor-derived Regulatory Molecules by Suppressing STAT3 Signaling to Promote Tumor ImmunityPubMed: 25962106
  • Interleukin 35 Synovial Fluid Levels Are Associated with Disease Activity of Rheumatoid ArthritisPubMed: 26204444
  • Interleukin-35 is upregulated in systemic sclerosis and its serum levels are associated with early diseasePubMed: 26231346
  • Anti-inflammatory effects of interleukin-35 in acquired aplastic anemiaPubMed: 26282938
  • Interleukin-35 Inhibits Endothelial Cell Activation by Suppressing MAPK-AP-1 PathwayPubMed: 26085094
  • Interleukin 35 may contribute to the loss of immunological self‐tolerance in patients with primary immune thrombocytopeniaPubMed: 25640666
  • New insights into immune mechanisms underlying response to Rituximab in patients with membranous nephropathy: A prospective study and a review of the literaturePubmed:26876383
  • Increased plasma concentrations of interleukin 35 in patients with coronary artery diseaseJournal:-19
  • The clinical utility of serum IL-35 in patients with polymyositis and dermatomyositispubmed:27502600
  • Enhanced LPS-induced activation of IL-27 signalling in sarcoidosispubmed:27492538
  • Effects of an intravitreal injection of interleukin-35-expressing plasmid on pro-inflammatory andanti-inflammatory cytokines.pubmed:27460435
  • IL-35在牙周炎及口腔扁平苔藓中的表达及相关性分析QK:98604X
  • Upregulation of Interleukin 35 in Patients With Endometriosis Stimulates Cell Proliferationpubmed:28659009
  • IL‑35 may maintain homeostasis of the immune microenvironment in periodontitisissn:2542-3975
  • Synoviocytes-derived Interleukin 35 Potentiates B Cell Response in Patients with Osteoarthritis and Rheumatoid Arthritispubmed:29247146
  • Human immunodeficiency virus type-1 induces a regulatory B cell-like phenotype in vitropubmed:28713164
  • Lower level of IL‑35 and its reduced inhibition in Th17 cells in patients with bone marrow mononuclear cells Coombs test‑positive hemocytopenia.pubmed:29257310
  • Evaluation of serum interleukin- 35 level in children with persistent asthma.pubmed:27996283
  • Interleukin-35 mitigates the function of murine transplanted islet cells via regulation of Treg/Th17 ratiopubmed:29236782
  • Significant association between FOXP3 gene polymorphism and steroid-resistant acute rejection in living donor liver transplantation10.1002/hep4.1052
  • Clinical significance of IL-35 expression in the progression of clear cell renal cell carcinoma ISSN:1940-5901
  • Interleukin-35 as a predictor of prostate cancer in patients undergoing initial prostate biopsy.pubmed:28761357
  • AB0734 Serum il-35 levels in systemic sclerosis and relationship with clinical features10.1136:annrheumdis-2018-eular.2605
  • Interleukin-35 as a New Biomarker of Renal Involvement in Lupus Nephritis PatientsPubmed:29576585
  • The impact of plasma SOCS3 levels and endometrial leukocytes on unexplained infertilityPubmed:29914245
  • Pro-tumoral immune cell alterations in wild type and Shb-deficient mice in response to 4T1 breast carcinomasPubmed:29721156
  • Serum Interleukin 35 Levels in Systemic Sclerosis and Relationship With Clinical Features.Pubmed: 30431486
  • Interleukin‑35 is associated with the tumorigenesis and progression of prostate cancer
  • Plasmacytoid dendritic cells protect against immune-mediated acute liver injury via IL-35Pubmed: 31264967
  • CD4+ FOXP3+ T Cells in Rheumatoid Arthritis Bone Marrow Are Partially ImpairedPubmed: 32111105
  • Interleukin-35 in idiopathic inflammatory myopathiesPubmed: 33128920
  • IL35 predicts prognosis in gastric cancer and is associated with angiogenesis by altering TIMP1, PAI1, and IGFBP1Pubmed: 33064893
  • Interleukin-35 promotes progression of prostate cancer and inhibits anti-tumour immunityPubmed: 33041668
  • Interleukin-9 regulates macrophage activation in the progressive multiple sclerosis brainPubmed: 32375811
  • Association of IL-35 expression and gene polymorphisms in rheumatoid arthritis33307515
  • Decreased interleukin-35 levels and CD4+ EBI3+ T cells in patients with type 1 diabetes and the effects of the antibody against CD20 (rituximab)33488880
  • Anti-inflammatory roles of interleukin-35 in the pathogenesis of Japanese cedar pollinosis34386410
  • Pentosan Polysulfate Sodium augments the therapeutic effect of 5-Aminosalicylic Acid in DSS colitis model; the role of IL-35 expressionPubmed:35247859
  • Association of three single nucleotide polymorphisms of interleukin-35 for detection and prediction of women with breast cancer in Erbil city
  • Analysis of IL-10 and IL-35 in DPP-4 inhibitor-related bullous pemphigoid

Recommend products