ELISA Kit DIY Materials for Ischemia Modified Albumin (IMA)
- UOM
- FOB US$ 1,166.00 US$ 1,944.00 US$ 3,499.00 US$ 6,804.00 US$ 11,664.00
- Quantity
Overview
Properties
- Product No.KSA825Mu11
- Organism SpeciesMus musculus (Mouse) Same name, Different species.
- ApplicationsMain materials for "Do It (ELISA Kit) Yourself".
Research use only - Downloadn/a
- CategoryMetabolic pathwayCardiovascular biology
- Reagent Contents Capture Antibody, Biotin-labeled Competitor, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
- Detectable SampleSerum, plasma and other biological fluids.
- Applicable PrincipleCompetitive Inhibition ELISA for Antigen Detection
- Detectable Range370.4-30,000ng/mL
- Applicable Sensitivity135.2ng/mL
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Specifity
The Abs in the kit have high sensitivity and excellent specificity for detection of Ischemia Modified Albumin (IMA). No significant cross-reactivity or interference between Ischemia Modified Albumin (IMA) and analogues was observed.
Usage
1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.
Storage
Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.
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Citations
- The role of protein oxidation and DNA damage in elderly hypertensionPubMed: 26487663
- The impact of protein oxidation on sustained and white coat hypertensionpubmed:27684518
- Investigation of serum ischemia-modified albumin levels in coronary artery disease patients
- Effects of Different Heart Positions During Off-pump Coronary Artery Bypass Surgery on Serum Ischemia Modified Albumin Level and Cardiac Output