ELISA Kit DIY Materials for Jagged 1 (JAG1)
CD339; AGS; AHD; AWS; HJ1; JAGL1; Alagille Syndrome; Jagged 1 Protein
- UOM
- FOB US$ 1,197.00 US$ 1,995.00 US$ 3,591.00 US$ 6,983.00 US$ 11,970.00
- Quantity
Overview
Properties
- Product No.KSB807Hu01
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- ApplicationsMain materials for "Do It(ELISA Kit) Yourself"
Research use only - Downloadn/a
- CategorySignal transductionCD & Adhesion moleculeTumor immunityHematology
- Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
- Detectable SampleSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
- Detectable Range0.156-10ng/mL
- Applicable Sensitivity0.057ng/mL
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Specifity
The Abs in the kit have high sensitivity and excellent specificity for detection of Jagged 1 Protein (JAG1). No significant cross-reactivity or interference between Jagged 1 Protein (JAG1) and analogues was observed.
Usage
1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.
Storage
Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.
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Citations
- Cross-talk between the Toll-like receptor 4 and Notch1 pathways augments the inflammatory response in the interstitial cells of stenotic human aortic valvesPubMed: PMC3506187
- Profiling of selected angiogenesis-related genes in proliferative eutopic endometrium of women with endometriosisPubmed: 24188612
- Augmented Osteogenic Responses in Human Aortic Valve Cells Exposed to oxLDL and TLR4 Agonist: A Mechanistic Role of Notch1 and NF-κB InteractionPubmed:Pmc4014478
- Ligation of ICAM-1 on human aortic valve interstitial cells induces the osteogenic response: A critical role of the Notch1-NF-κB pathway in BMP-2 expressionPubmed:25101972
- Jagged1 Stimulates Bone Resorption during Orthodontic Tooth MovementDn: Jlc
- MicroRNA‑26b acts as an antioncogene and prognostic factor in cervical cancer
- Growth hormone induces Notch1 signaling in podocytes and contributes to proteinuria in diabetic nephropathyPubmed: 31511328
- Growth hormone induces mitotic catastrophe of glomerular podocytes and contributes to proteinuria33795655