ELISA Kit DIY Materials for Kisspeptin 1 (KISS1) 
Metastin; KiSS-1 Metastasis-Suppressor
- UOM
- FOB US$ 1,260.00 US$ 2,100.00 US$ 3,780.00 US$ 7,350.00 US$ 12,600.00
- Quantity
Overview
Properties
- Product No.KSC559Hu01
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- ApplicationsMain materials for "Do It (ELISA Kit) Yourself".
Research use only - Downloadn/a
- CategoryTumor immunity
- Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
- Detectable SampleSerum, plasma and other biological fluids.
- Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
- Detectable Range31.2-2000pg/mL
- Applicable Sensitivity13.1pg/mL
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Packages (Simulation)
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Packages (Simulation)
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ISO9001: 2008, ISO13485: 2003 Registered
Specifity
The Abs in the kit have high sensitivity and excellent specificity for detection of Kisspeptin 1 (KISS1). No significant cross-reactivity or interference between Kisspeptin 1 (KISS1) and analogues was observed.
Usage
1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.
Storage
Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.
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Citations
- LIM-homeodomain transcription factor Isl-1 mediates kisspeptin's effect on insulin secretion in mice.Pubmed:24956377
- 双酚A 与女童性早熟的关系Fileup: Pdf
- The relation of urinary bisphenol A with kisspeptin in girls diagnosed with central precocious puberty and premature thelarchePubMed: 26565542
- Circulating levels of leptin, nesfatin-1 and kisspeptin in postmenopausal obese womenPubmed:27011110
- The Changes of Serum Leptin and Kisspeptin Levels in Chinese Children and Adolescents in Different Pubertal Stagespubmed:27990162
- Basal Serum Neurokinin B Levels in Differentiating Idiopathic Central Precocious Puberty from Premature Thelarche.pubmed:28008860
- Kisspeptin Protein in Seminal Plasma Is Positively Associated with Semen Quality: Results from the MARHCS Study in Chongqing, China
- Lack of Improvement of Sperm Characteristics in Obese Males After Obesity Surgery Despite the Beneficial Changes Observed in Reproductive HormonesPubmed: 30806913
- Serum Kisspeptin and Its Relation to Metabolic Parameters and Glucose Metabolism in Prepubertal and Pubertal Obese ChildrenPubmed: 33224197
- KISS1R Signaling Modulates Gonadotropin Sensitivity in Mouse Leydig CellPubmed: 33112765
- Beyond GnRH, LH and FSH: the role of kisspeptin on hypothalalmic-3 pituitary gonadal (HPG) axis pathology and diagnostic consideration 4
- Kisspeptin and Hematologic Parameters as Predictive Biomarkers for First-Trimester Abortions34239761
- Comparison of the Kisspeptin levels in early onset preeclampsia and late-onset preeclampsiaPubmed:35039882
- Serum kisspeptin, leptin, neuropeptide Y, and neurokinin B levels in adolescents with polycystic ovary syndromePubmed:35170267
- Differential Expression of Kisspeptin System and Kisspeptin Receptor Trafficking during Spermatozoa Transit in the EpididymisPubmed:35205340