ELISA Kit DIY Materials for Klotho (KL)
KLa; Klotho Alpha
- UOM
- FOB US$ 1,296.00 US$ 2,160.00 US$ 3,888.00 US$ 7,560.00 US$ 12,960.00
- Quantity
Overview
Properties
- Product No.KSH757Mu01
- Organism SpeciesMus musculus (Mouse) Same name, Different species.
- ApplicationsMain materials for "Do It(ELISA Kit) Yourself"
Research use only - Downloadn/a
- CategoryMetabolic pathway
- Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
- Detectable SampleSerum, plasma, tissue homogenates and other biological fluids.
- Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
- Detectable Range3.12-200pg/mL
- Applicable Sensitivity1.25pg/mL
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Specifity
The Abs in the kit have high sensitivity and excellent specificity for detection of Klotho (KL). No significant cross-reactivity or interference between Klotho (KL) and analogues was observed.
Usage
1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.
Storage
Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.
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Citations
- High phosphate feeding induced arterial medial calcification in uremic rats: Roles of Lanthanum carbonate on protecting vasculaturePubmed: 24012609
- Sick Sinus Syndrome May Attribute to the Decrease of ?±-klotho Protein in MiceCom:Source
- α-Klotho is an acute phase protein and altered by restraint stress in micePubmed:Pmc4203207
- Les biomarqueurs des calcifications vasculairesPubMed: 26069069
- Effect of secondary hyperparathyroidism serum on endothelial cells and intervention with KlothoPubMed: 25873020
- Human Preosteoblastic Cell Culture from a Patient with Severe Tumoral Calcinosis-Hyperphosphatemia Due to a NewGALNT3PubMed: 25899975
- 抗衰老蛋白Klotho 阻断高糖培养的大鼠肾小球系膜细胞TLR4/NF-κB p65/NGAL 通路的激活及其作用机制Cn: Article
- The function of miR-199a-5p/Klotho regulating TLR4/NF-κB p65/NGAL pathways in rat mesangial cells cultured with high glucose and the mechanismPubMed: 26419931
- Soluble Klotho protein as a novel serum biomarker in patients with acromegalypmc:PMC4754356
- Klotho Deficiency Aggravates Tacrolimus-Induced Renal Injury via the Phosphatidylinositol 3-Kinase-Akt-Forkhead Box Protein O PathwayPubmed:27174564
- Klotho preservation via histone deacetylase inhibition attenuates chronic kidney disease-associated bone injury in mice.pubmed:28387374
- Is there a role for newer biomarkers in chronic kidney disease-mineral and bone disorder management?pubmed:28429560
- Associations between serum fibroblast growth factor 23 level and intradialytic hypotension in hemodialysis patientsissn:2394-2916
- Protective effect of restructuring klotho protein combined with trimetazidine on myocardial injury in miceISSN:1936-2625
- Changes of Klotho protein and Klotho mRNA expression in a hydroxy-L-proline induced hyperoxaluric rat modelpubmed:28943597
- Klotho Reduces Necroptosis by Targeting Oxidative Stress Involved in Renal Ischemic-Reperfusion InjuryPubmed:29550818
- Advanced maternal age impairs spatial learning capacity in young adult mouse offspringPubmed:29636887
- Clinical implication of alterations in serum Klotho levels in patients with type 2 diabetes mellitus and its associated complicationsPubmed:30042059
- Klotho Inhibits Proliferation and Migration of Angiotensin II-Induced Vascular Smooth Muscle Cells (VSMCs) by Modulating NF-κB p65, Akt, and Extracellular Signal …Pubmed:30004089
- High-serum phosphate and parathyroid hormone distinctly regulate bone loss and vascular calcification in experimental chronic kidney diseasePubmed: 30189026
- The role of klotho in chronic kidney diseasePubmed: 30348110
- Age-related declines in α-Klotho drive progenitor cell mitochondrial dysfunction and impaired muscle regenerationPubmed: 30451844
- Wnt/β-catenin signaling mediates both heart and kidney injury in type 2 cardiorenal syndromePubmed: 30770217
- The use of LP533401 as a therapeutic option for renal osteodystrophy affects, renal calcium handling, vitamin D metabolism and bone health in uremic ratsPubmed: 30801205
- CD317 mediates immunocytolysis resistance by RICH2/cytoskeleton-dependent membrane protectionPubmed: 33223223
- Effects of salt intervention on serum levels of Klotho influenced by salt sensitivityPubmed: 33164306
- Expression of α-Klotho is downregulated and associated with oxidative stress in the lens in streptozotocin-induced diabetic ratsPubmed: 32744464
- Klotho ameliorates the onset and progression of cataract via suppressing oxidative stress and inflammation in the lens in streptozotocin-induced diabetic ratsPubmed: 32438076
- P2X7 siRNA targeted to the kidneys increases klotho and delays the progression of experimental diabetic nephropathy.Pubmed: 32377919
- Ameliorating Effect of Klotho Protein on Rat Heart during I/R InjuryPubmed: 33123314
- Increased Plasma Level of Longevity Protein Klotho as a Potential Indicator of Cognitive Function Preservation in Patients With SchizophreniaPubmed: 32612508
- ¦ÁKlotho protein has therapeutic activity in contrast-induced acute kidney injury by limiting NLRP3 inflammasome-mediated pyroptosis and promoting autophagy33675964
- Investigation of the Role of Induced Overexpression of the Isolated Secreted Klotho on the A-172 Human Glioblastoma CellsPubmed:35112226
- Expression of Klotho in Follicles of Patients With Polycystic Ovary Syndrome and Its Clinical Significance
- Klotho is a prognostic indicator of oocytes qualities in polycystic ovary syndrome