ELISA Kit DIY Materials for Lysophosphatidylcholine (LPC)

lysoPC; Lysolecithin; 1-Palmitoyl-sn-Glycero-3-Phosphocholine

UOM
1. 96T*5
2. 96T*10
3. 96T*20
4. 96T*50
5. 96T*100
FOB US$ 1,469.00 US$ 2,448.00 US$ 4,406.00 US$ 8,568.00 US$ 14,688.00
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Overview

Properties

Product No.KSK621Ge11
Organism SpeciesPan-species (General) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Canine
- Porcine
- Bovine
- Caprine
- Multi-species
- Pan-species
- Others
ApplicationsMain materials for "Do It (ELISA Kit) Yourself".
Research use only
DownloadInstruction Manual
Category

Reagent Contents Capture Antibody, Biotin-labeled Competitor, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
Detectable SampleSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Applicable PrincipleCompetitive Inhibition ELISA for Antigen Detection
Detectable Range246.9-20,000ng/mL
Applicable Sensitivity89.3ng/mL

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Packages (Simulation)
Packages (Simulation)

ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The Abs in the kit have high sensitivity and excellent specificity for detection of Lysophosphatidylcholine (LPC). No significant cross-reactivity or interference between Lysophosphatidylcholine (LPC) and analogues was observed.

Usage

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

Storage

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

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Citations

High-fat diet-induced acceleration of osteoarthritis is associated with a distinct and sustained plasma metabolite signaturepubmed:28811491
Iron Homeostasis Determines Fate of Human Pluripotent Stem Cells Via Glycerophospholipids‐Epigenetic CircuitPubmed: 30599084
Associations between plasma lysophospholipids concentrations, chronic kidney disease and the type of renal replacement therapyPubmed: 30947711
ER-residential Nogo-B accelerates NAFLD-associated HCC mediated by metabolic reprogramming of oxLDL lipophagyPubmed: 31358770
Dietary Betaine Addition Promotes Hepatic Cholesterol Synthesis, Bile Acid Conversion, and Export in RatsPubmed: 32414094
iPLA2¦Â Contributes to ER Stress-Induced Apoptosis during Myocardial Ischemia/Reperfusion Injury
Downregulation of activating transcription factor 4 attenuates lysophosphatidycholine-induced inflammation via the NF-κB pathway34582847
Astragaloside IV Inhibits Bleomycin-Induced Ferroptosis in Human Umbilical Vein Endothelial Cells by Mediating LPC34760046