ELISA Kit DIY Materials for Macrophage Migration Inhibitory Factor (MIF)

GIF; GLIF; MMIF; Glycosylation-Inhibiting Factor; L-dopachrome isomerase; L-dopachrome tautomerase; Phenylpyruvate tautomerase

UOM
1. 96T*5
2. 96T*10
3. 96T*20
4. 96T*50
5. 96T*100
FOB US$ 1,134.00 US$ 1,890.00 US$ 3,402.00 US$ 6,615.00 US$ 11,340.00
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Overview

Properties

Product No.KSA698Hu01
Organism SpeciesHomo sapiens (Human) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Canine
- Porcine
- Bovine
- Caprine
- Multi-species
- Pan-species
- Others
ApplicationsMain materials for "Do It (ELISA Kit) Yourself".
Research use only
Downloadn/a
CategoryCytokine Infection immunity Rheumatology Autoimmunity

Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
Detectable SampleSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
Detectable Range0.312-20ng/mL
Applicable Sensitivity0.128ng/mL

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Packages (Simulation)
Packages (Simulation)

ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The Abs in the kit have high sensitivity and excellent specificity for detection of Macrophage Migration Inhibitory Factor (MIF). No significant cross-reactivity or interference between Macrophage Migration Inhibitory Factor (MIF) and analogues was observed.

Usage

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

Storage

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

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Citations

Targeted reduction of advanced glycation improves renal function in obesityPubMed: 21412218
Macrophage Migration Inhibitory Factor Plays a Role in the Regulation of Microfold (M) Cell-Mediated Transport in the GutJimmunol: 5673
Involvement of exercise-induced macrophage migration inhibitory factor in the prevention of fatty liver diseasePubMed: PMC3757527
Role of macrophage migration inhibitory factor in the regulatory T cell response of tumor-bearing micePubMed: PMC3466372
Deletion of bone-marrow-derived receptor for AGEs (RAGE) improves renal function in an experimental mouse model of diabetesPubmed:24957662
The Potential Role of Polymethyl Methacrylate as a New Packaging Material for the Implantable Medical Device in the BladderPubMed: 25705692
Combined NOX1/4 inhibition with GKT137831 in mice provides dose-dependent reno- and atheroprotection even in established micro- and macrovascular disease.pubmed:28160092
Protective effect of chlorogenic acid on the inflammatory damage of pancreas and lung in mice with l-arginine-induced pancreatitis pubmed:28919396
Cytokine MIF Enhances Blood-Brain Barrier Permeability: Impact for Therapy in Ischemic StrokePubmed:29335619
Immune Response and Mechanisms of IFN-γ in Administration for KeratomycosisPubmed: 30307777
Effect of voluntary running on expression of myokines in brains of rats with depressionPubmed: 30834799
Biomarkers in Heart Failure and Associated Diseases
Autophagy mediates the secretion of macrophage migration inhibitory factor from cardiomyocytes upon serum-starvationPubmed: 31209799
Beneficial effects of voluntary over forced exercise on skeletal muscle structure and myokines expressionPubmed: 31802473
Deletion of macrophage migration inhibitory factor ameliorates inflammation in mice model severe acute pancreatitisPubmed: 32062385
Association between MIF gene promoter rs755622 and susceptibility to coronary artery disease and inflammatory cytokines in the Chinese Han population33850223
Macrophage migration inhibitory factor is overproduced through EGR1 in TET2low resting monocytesPubmed:35115654