ELISA Kit DIY Materials for Malondialdehyde (MDA) Pan-species (General) Assay Kit DIY Materials

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Overview
Properties
  • Reagent Contents Capture Antibody, Biotin-labeled Competitor, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable Sampleserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
  • Applicable PrincipleCompetitive Inhibition ELISA for Antigen Detection
  • Detectable Range24.69-2,000ng/mL
  • Applicable Sensitivity9.15ng/mL
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  • ELISA Kit DIY Materials for Malondialdehyde (MDA) Packages (Simulation)
  • ELISA Kit DIY Materials for Malondialdehyde (MDA) Packages (Simulation)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The Abs in the kit have high sensitivity and excellent specificity for detection of Malondialdehyde (MDA). No significant cross-reactivity or interference between Malondialdehyde (MDA) and analogues was observed.

Usage

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

Storage

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

Support pack

Citations

  • Lipopolysaccharide pretreatment protects against ischemia/reperfusion injury via increase of HSP70 and inhibition of NF-κBPubMed: 21562784
  • Effect of intermittent hepatic inflow occlusion with the Pringle maneuver during donor hepatectomy in adult living donor liver transplantation with right hemiliver grafts: a prospective, randomized controlled studyPubMed: 21837746
  • Formaldehyde Impairs Learning and Memory Involving the Disturbance of Hydrogen Sulfide Generation in the Hippocampus of RatsPubMed: 23108488
  • The amelioration of AH by abstinence and the attenuation of oxidative stressPubMed: 21940381
  • High salt intake accelerated cardiac remodeling in spontaneously hypertensive rats: time window of left ventricular functional transition and its relation to salt-loading dosesPubMed: 21529316
  • Effect of caffeic acid on doxorubicin induced cardiotoxicity in ratsAjbm: Source
  • Iodinated contrast media can induce long-lasting oxidative stress in hemodialysis patients.Pubmed: 24142649
  • Chronic thromboembolic pulmonary hypertension is not associated with iron overloadPubmed:25449047
  • Oxidative stress status in elite athletes engaged in different sport disciplinesPubmed:Pmc4333958
  • Role of Aldehyde Dehydrogenase 2 in 1-Methy-4-Phenylpyridinium Ion-Induced Aldehyde Stress and Cytotoxicity in PC12 CellsPubmed:25005621
  • Ischemic postconditioning relieves cerebral ischemia and reperfusion injury through activating T-LAK cell-originated protein kinase/protein kinase B pathway in rats.Pubmed:25013016
  • Metformin alleviates high glucosePubMed: 25760137
  • Influence of Malondialdehyde and Matrix Metalloproteinase-9 on Progression of Carotid Atherosclerosis in Chronic Renal Disease with Cardiometabolic SyndromePubMed: 26538831
  • BDNF-TrkB Pathway Mediates Neuroprotection of Hydrogen Sulfide against Formaldehyde-Induced Toxicity to PC12 CellsPubMed: 25749582
  • Influence of Malondialdehyde and Matrix Metalloproteinase-9 on Progression of Carotid Atherosclerosis in Chronic Renal Disease with Cardiometabolic Syndrome PubMed: 26538831
  • Mitochondrial respiratory dysfunctions of blood mononuclear cells link with cardiac disturbance in patients with early-stage heart failurePubMed: 26018291
  • Obstructive sleep apnea is independently associated with inflammation and insulin resistance, but not with blood pressure,PubMed: 26429654
  • Puerarin Attenuates Anoxia/Reoxygenation Injury Through Enhancing Bcl-2 Associated Athanogene 3 Expression, a Modulator of Apoptosis and AutophagyPubmed:27011313
  • Mechanism (s) of action underlying the gastroprotective effect of ethyl acetate fraction obtained from the crude methanolic leaves extract of Muntingia calaburaPubmed:26912079
  • Evaluation of hepatorenal impairments in Wistar rats coexposed to low-dose lead, cadmium and manganese: insights into oxidative stress mechanismpubmed:27599793
  • Hydrogen Sulfide Ameliorates Homocysteine-Induced Cognitive Dysfunction by Inhibition of Reactive Aldehydes Involving Upregulation of ALDH2pubmed:27988490
  • Comparative Analysis of Some Antioxidant Markers in Periodontal Diseasepdf:MARTU%207%2016.pdf
  • Influence of Supplementing Diet with Microalgae (Schizochytrium limacinum) on Growth and Metabolism of Lambs during the Summer 2180761
  • Renal Denervation Attenuates Multi-Organ Fibrosis and Improves Vascular Remodeling in Rats with Transverse Aortic Constriction Induced Cardiomyopathypubmed:27889753
  • Panax notoginseng saponins protect kidney from diabetes by up-regulating silent informationregulator 1 and activating antioxidant proteins in rats.pubmed:26712211
  • Hydrogen sulfide inhibits MPP+-induced aldehyde stress and endoplasmic reticulum stress inPC12 cells: involving upregulation of BDNF.pubmed:27641114
  • Transcriptional abundance of antioxidant enzymes in endometrium and their circulating levels in Zebu cows with and without uterine infection.pubmed:28007408
  • Effect of Indole-3-carbinol and/or Metformin on Female Patients with Ulcerative Colitis (Premalignant Condition): Role of Oxidative Stress, Apoptosis and Proinflammatory Cytokinesjcrt:5
  • Inhibition of ALDH2 protects PC12 cells against formaldehyde-induced cytotoxicity: involving the protection of hydrogen sulphide.pubmed:28251688
  • Influence of supplementing diet with microalgae (Schizochytrium limacinum) on growth and metabolism in lambs during the summer795296
  • Hydrogen sulfide attenuates chronic restrain stress-induced cognitive impairment by upreglulation of Sirt1 in hippocampuspubmed:29245987
  • Resveratrol attenuates type 2 diabetes mellitus by mediating mitochondrial biogenesis and lipid metabolism via Sirtuin type 110.3892/etm.2017.5400
  • Changes in the concentrations of inflammatory and oxidative status biomediators (MIP-1 α, PMN elastase, MDA, and IL-12) in depressed patients with and without posttraumatic stress disorder10.1016/j.pharep.2017.08.008
  • Sex differences in complex regional pain syndrome type I (CRPS-I) in micepubmed:28831269
  • Dyslipdemia induced by chronic low dose co-exposure to lead, cadmium and manganese in rats: the role of oxidative stresspubmed:28654832
  • Burn Serum Increases Staphylococcus aureus Biofilm Formation via Oxidative Stress.pubmed:28702016
  • Biochemical and molecular alterations in freshwater mollusks as biomarkers for petroleum product, domestic heating oilPubmed:29660615
  • Epigallocatechin-3-Gallate Reduces Neuronal Apoptosis in Rats after Middle Cerebral Artery Occlusion Injury via PI3K/AKT/eNOS Signaling PathwayPubmed:29770336
  • Gold Nanoparticle Toxicity in Mice and Rats: Species DifferencesPubmed:29742986
  • Protective effects of N (2)‑L‑alanyl‑L‑glutamine mediated by the JAK2/STAT3 signaling pathway on myocardial ischemia reperfusionPubmed:29393473
  • Hydrogen‑rich solution against myocardial injury and aquaporin expression via the PI3K/Akt signaling pathway during cardiopulmonary bypass in ratsPubmed:29956781
  • Epigallocatechingallate attenuates myocardial injury in a mouse model of heart failure through TGF‑β1/Smad3 signaling pathwayPubmed:29620209
  • Hydrogen‑rich solution attenuates myocardial injury caused by cardiopulmonary bypass in rats via the Janus‑activated kinase 2/signal transducer and activator of …Pubmed:29928398
  • Multi-biomarker responses after exposure to organophosphates chlorpyrifos in the freshwater mussels and snails10.1080:10807039.2018.1460800
  • Effects of ginsenoside Rb1 on oxidative stress injury in rat spinal cords by regulating the eNOS/Nrf2/HO‑1 signaling pathwayPubmed:30116359
  • Lead-induced increase in erythrocyte osmotic fragility and malondialdehyde concentration in Wistar rats: Chemo-protective effects of flavonoid mixture2018/1/17-021.pdf
  • Enoxaparin attenuates doxorubicin induced cardiotoxicity in rats via interfering with oxidative stress, inflammation and apoptosisPubmed:29321061
  • Are thalassemia patients oxidatively challenged?10.1016:j.mjafi.2017.12.002
  • Serum Uric Acid Levels are Associated with Cardiometabolic Risk Factors in Healthy Young and Middle-Aged AdultsPubmed: 30328946
  • Hydrogen peroxide-induced mitophagy contributes to laryngeal cancer cells survival via the upregulation of FUNDC1Pubmed: 30284230
  • Hepatoprotective activity of Erythrina× neillii leaf extract and characterization of its phytoconstituentsPubmed: 28095910
  • Improved endogenous epoxyeicosatrienoic acid production mends heart function via increased PGC 1α-mitochondrial functions in metabolic syndromePubmed: 30342783
  • Probucol improves erectile function via Activation of Nrf2 and coordinates the HO-1/DDAH/PPAR-γ/eNOS pathways in streptozotocin-induced diabetic ratsPubmed: 30454888
  • Effects of Tualang honey in modulating nociceptive responses at the spinal cord in offspring of prenatally stressed ratsDoi: 10.1016/j.joim.2018.12.002
  • Protective Effects of Uncultured Adipose‐Derived Stromal Vascular Fraction on Testicular Injury Induced by Torsion‐Detorsion in RatsPubmed: 30569668
  • Naomaitai Ameliorated Brain Damage in Rats with Vascular Dementia by PI3K/PDK1/AKT Signaling Pathway
  • Examination of Oxidative Stress Markers in Women with Postmenopausal Osteoporosis
  • The Relationship Between Serum Level of Malondialdehyde and Progression of Colorectal Cancer
  • The effects of hydrogen-rich saline solution on intestinal anastomosis performed after intestinal ischemia reperfusion injury
  • European Journal of Biomedical AND Pharmaceutical sciences
  • The cardiovascular phenotype of adult patients with phenylketonuriaPubmed: 31492166
  • Hydrogen Sulfide Ameliorates Cognitive Dysfunction in Formaldehyde-Exposed Rats: Involvement in the Upregulation of Brain-Derived Neurotrophic FactorPubmed: 31550727
  • Influence of deficient nutrition on trace element status and antioxidant defense systemPubmed: 1781925
  • Ginsenoside Rg3-loaded, reactive oxygen species-responsive polymeric nanoparticles for alleviating myocardial ischemia-reperfusion injuryPubmed: 31783047
  • Phenolic Compounds and the Anti-Atherogenic Effect of Bee Bread in High-Fat Diet-Induced Obese RatsPubmed: 31905919
  • Hydrogen Sulfide Prevents Sleep Deprivation-Induced Hippocampal Damage by Upregulation of Sirt1 in the HippocampusPubmed: 32218719
  • NRF2 deficiency increases obesity susceptibility in a mouse menopausal modelPubmed: 32045430
  • Selected Oxidative Stress Markers in Colorectal Cancer Patients in Relation to Primary Tumor Location—A Preliminary ResearchPubmed: 31972987
  • Exosomes of Antler Mesenchymal Stem Cells Improve Postoperative Cognitive Dysfunction in Cardiopulmonary Bypass Rats through Inhibiting the TLR2 …Pubmed: 32300366
  • NFKB1 gene rs28362491 ins/del variation is associated with higher susceptibility to myocardial infarction in a Chinese Han populationPubmed: 33177541
  • Rapamycin improves renal injury induced by Iodixanol in diabetic rats by deactivating the mTOR/p70S6K signaling pathwayPubmed: 32798557
  • Improvement of Endothelial Dysfunction of Berberine in Atherosclerotic Mice and Mechanism Exploring through TMT-Based ProteomicsPubmed: 32566106
  • Effects of microencapsulated probiotics and plant extract on antioxidant ability, immune status and caecal microflora in Escherichia coli K88-challenged broiler …
  • Possible Role of Statins on the Inflammatory Biomarkers in Patients With Periodontal Disease: A Cross-Sectional Study
  • Tualang honey improves memory and hippocampal changes in adult offspring of prenatally stressed rats
  • Effects of Tualang Honey on Pain Behaviour and Oxidative Stress in the Thalamus of Prenatally Stressed Rat Offspring
  • The Anticarcinogenic Effect of the Apple Polyphenol Phloretin in an Experimental Rat Model of Hepatocellular Carcinoma
  • Formaldehyde induces ferroptosis in hippocampal neuronal cells by upregulation of the Warburg effect33259821
  • Tualang Honey Improves Memory and Prevents Hippocampal Changes in Prenatally Stressed Rats33389951
  • Endostatin attenuates heart failure via inhibiting reactive oxygen species in myocardial32686821
  • Dental pulp stem cells overexpressing hepatocyte growth factor facilitate the repair of DSS-induced ulcerative colitis33413675
  • Protective effects of hydrogen rich saline solution on ventral penile Mathieu type flap with penile tourniquet application in rats33608226
  • Tanshinone IIA attenuates heart failure via inhibiting oxidative stress in myocardial infarction rats33786621
  • Alarin alleviated cardiac fibrosis via attenuating oxidative stress in heart failure rats34089389
  • Naringenin alleviates myocardial ischemia reperfusion injury by enhancing the myocardial miR-126-PI3K/AKT axis in streptozotocin-induced diabetic rats34093766
  • MicroRNA-145-5p aggravates cell apoptosis and oxidative stress in tongue squamous cell carcinoma33732346
  • The Influence of Probiotic Supplementation on Depressive Symptoms, Inflammation and Oxidative Stress Parameters and Faecal Microbiota in Patients with Depression Depending on Metabolic Syndrome Comorbidity ¨C PRO-DEMET Randomised Study Protocol33804999
  • κ-Opioid Receptor Agonist Ameliorates Postoperative Neurocognitive Disorder by Activating the Ca2+/CaMKII/CREB Pathway34608407
  • Alamandine alleviates hypertension and renal damage via oxidative-stress attenuation in Dahl ratsPubmed:35022384
  • Peripheral blood oxidative stress markers for obstructive sleep apnea—a meta-analysisPubmed:34981298
  • Intake of Bifidobacterium lactis Probio-M8 fermented milk protects against alcoholic liver diseasePubmed:35086715
  • Fibroblast growth factor 12 attenuated cardiac remodeling via suppressing oxidative stressPubmed:35304156
  • Targeting of midkine alleviates cardiac hypertrophy via attenuation of oxidative stress and autophagyPubmed:35427698
  • Antioxidant Effect of Tyr-Ala Extracted from Zein on INS-1 Cells and Type 2 Diabetes High-Fat-Diet-Induced MicePubmed:35740008
  • The κ-Opioid Receptor Agonist U50488H Ameliorates Neuropathic Pain Through the Ca2+/CaMKII/CREB Pathway in RatsPubmed:35645576
  • Malondialdehyde and Neutrophil Gelatinase-Associated Lipocalin as Markers of Oxidative Stress in Small for Gestational Age Newborns from Hypertensive …Pubmed:35224102
  • Association of Oxidative Stress Markers with Vascular Stiffness Parameters in Patients with Diabetic Neuropathy
  • Transplanted hair follicle mesenchymal stem cells alleviated small intestinal ischaemia–reperfusion injury via intrinsic and paracrine mechanisms in a rat model

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