ELISA Kit DIY Materials for Mannose Binding Lectin (MBL) Chicken (Gallus) Assay Kit DIY Materials

MBL2; COLEC1; HSMBPC; MBP1; MBP; Collectin-1; Mannose-binding protein C; Mannan Binding Protein; Mannose-Binding Lectin(Protein C)2,Soluble(Opsonic Defect)

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Overview
Properties
  • Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable SampleSerum, plasma and other biological fluids.
  • Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
  • Detectable Range1.56-100ng/mL
  • Applicable Sensitivity0.57ng/mL
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  • ELISA Kit DIY Materials for Mannose Binding Lectin (MBL) Packages (Simulation)
  • ELISA Kit DIY Materials for Mannose Binding Lectin (MBL) Packages (Simulation)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The Abs in the kit have high sensitivity and excellent specificity for detection of Mannose Binding Lectin (MBL). No significant cross-reactivity or interference between Mannose Binding Lectin (MBL) and analogues was observed.

Usage

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

Storage

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

Support pack

Citations

  • Analysis of the Urine Proteome of Human Contrast-Induced Kidney Injury Using Two-Dimensional Fluorescence Differential Gel Electrophoresis/Matrix-Assisted Laser Desorption Time-of-Flight Mass Spectrometry/Liquid Chromatography Mass Spectrometry. Karger: 000255439
  • Serum Levels and H/L Gene Polymorphism of Mannose-Binding Lectin in Primary Open Angle GlaucomaIngenta: art00007
  • Complement activation contributes to the injury and outcome of kidney in human anti-glomerular basement membrane disease.PubMed: 22941511
  • Urinary mannose-binding lectin is a biomarker for predicting the progression of immunoglobulin (Ig)A nephropathyPubMed: PMC3406374
  • Alternative Complement Pathway Activation Products in Urine and Kidneys of Patients with ANCA-Associated GNPubmed: 24115193
  • Novel findings on the role of ficolins and colectins in the innate response against Leishmania braziliensisPubmed: 32827456
  • Complement activation profile of patients with primary focal segmental glomerulosclerosisPubmed: 32569286
  • The lectin pathway of complement and the initial recognition of Leishmania infantum promastigotes34242658

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