ELISA Kit DIY Materials for Melatonin (MT) Pan-species (General) Assay Kit DIY Materials

N-Acetyl-5-Methoxytryptamine

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Overview
Properties
  • Reagent Contents Capture Antibody, Biotin-labeled Competitor, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable SampleSerum, plasma and other biological fluids.
  • Applicable PrincipleCompetitive Inhibition ELISA for Antigen Detection
  • Detectable Range6.17-500pg/mL
  • Applicable Sensitivity2.76pg/mL
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  • ELISA Kit DIY Materials for Melatonin (MT) Packages (Simulation)
  • ELISA Kit DIY Materials for Melatonin (MT) Packages (Simulation)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The Abs in the kit have high sensitivity and excellent specificity for detection of Melatonin (MT). No significant cross-reactivity or interference between Melatonin (MT) and analogues was observed.

Usage

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

Storage

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

Support pack

Citations

  • Effects of melatonin on islet neogenesis and beta cell apoptosis in streptozotocin-induced diabetic rats: an immunohistochemical studyScienceDirect: S0739724012000112
  • Biomarkers inducing changes due to microwave exposure effect on rat brainAcademin: Source
  • Comparison of the flow characteristics for the honeycomb and labyrinth seal using numerical simulationCnki: Source
  • The therapeutic effect of a pulsed electromagnetic field on the reproductive patterns of male Wistar rats exposed to a 2.45-GHz microwave fieldPubMed: 21876981
  • Effects of simultaneous combined exposure to CDMA and WCDMA electromagnetic fields on serum hormone levels in ratsPubMed: 23239176
  • Improvement of oxidative stress and immunity by melatonin: An age dependent study in golden hamsterPubmed: 23220117
  • Melatonin and its correlation with testosterone in polycystic ovarian syndromeNCBI: PMC3963309
  • Daily variation in melatonin level, antioxidant activity and general immune response of peripheral blood mononuclear cells and lymphoid tissues of Indian goat Capra …Pubmed: 24851409
  • Serum Melatonin Level Disturbance is Related to Metabolic Syndrome and Subclinical Arterial Dysfunction in Shift Working Healthy MenResearchgate: Source
  • Melatonin Administration Aids Bone Healing in Diabetic RatsIjvm: Source
  • Photoperiodic induced melatonin regulates immunity and expression pattern of melatonin receptor MT1 in spleen and bone marrow mononuclear cells of male golden hamster.Pubmed: 24090924
  • Influence of?circadian?time?and lighting conditions on expression of melatonin?receptors?1?and?2?in?murinelymphocytes.Pubmed:25189896
  • Molecularly imprinted electrochemical sensing of urinary melatonin in a microfluidic system Pubmed:25584113
  • Localization and expression of androgen receptor (AR) and melatonin membrane receptor (MT1R) in accessory sex organs of adult male golden hamster, Mesocricetus auratus: Modulation by photoperiod.Com:Source
  • Titanium oxide (TiO2) nanoparticles in induction of apoptosis and inflammatory response in brainSpringer:Source
  • MT1 receptor expression and AA-NAT activity in lymphatic tissue following melatonin administration in male golden hamsterPubmed:25023124
  • Photoperiodic regulation of melatonin membrane receptor (MT1R) expression and steroidogenesis in testis of adult golden hamster, Mesocricetus auratusPubmed:25255424
  • Melatonin prevents dexamethasone-induced testicular oxidative stress and germ cell apoptosis in golden hamster, Mesocricetus auratusPubmed:25277062
  • Reproductive seasonality in the Indian pygmy field mouse, Mus terricolorTandfonline:Source
  • Developmental changes of melatonin receptor expression in the spleen of the chicken, Gallus domesticusPubMed: 26024931
  • Melatonin modulates monochromatic light-induced GHRH expression in the hypothalamus and GH secretion in chicksPubmed:26948666
  • Effect of extended photoperiod during winter on growth and onset of puberty in Murrah buffalo heifersPubmed:27051212
  • Effect of melatonin on monochromatic light-induced T-lymphocyte proliferation in the thymus of chickensPubmed:27203566
  • Effect of Monochromic Light-emitting Diode Light with Different Color on the Growth and ReproductivePerformances of Breeder Geese.Pubmed:26954165
  • Desempenho e comportamento de suínos em fase de terminação submetidos a diferentes programas de luzS1519-99402016000200272
  • Green light inhibits GnRH-I expression by stimulating the melatonin-GnIH pathway in the chick brain.pubmed:28295740
  • EFFECTS OF DIURNAL AND NOCTURNAL STRENUOUS EXERCISE ON SERUM MELATONIN LEVELSS1517-86922016000600436
  • Role of monochromatic light on daily variation of clock gene expression in the pineal gland ofchick.pubmed:27643985
  • Antioxidant effects of melatonin in heart tissue after induction of experimental periodontitis inrats.pubmed:28367898
  • 单色绿光对鸡胚褪黑激素合成的影响DOI: 10.11843/j.issn.0366-6964.2017.05.019
  • Melatonin Supplementation Lowers Oxidative Stress and Regulates Adipokines in Obese Patients on a Calorie-Restricted Dietpubmed:29142618
  • Inhibitory Effect of Melatonin on Formation of the Chicken Primordial Follicles.ISSN: 0253-8318
  • Melatonin modulates monochromatic light-induced melatonin receptor expression in the hypothalamus of chickspubmed:28943001
  • A Circadian Rhythm in both Complement Cascade (ComC) Activation and Sphingosine-1-Phosphate (S1P) Levels in Human Peripheral Blood Supports a …10.1007:s12015-018-9836-7
  • Effect of Monochromatic Light on Circadian Rhythm of Clock Genes in Chick PinealocytesPubmed:29896808
  • Expression of melatonin and its related synthase and membrane receptors in the oestrous corpus luteum and corpus luteum verum of sheepPubmed:29943511
  • OCCUPATIONAL HYGIENE–PERFORMANCE COFACTOR IN POWER ENGINEERING. BIOCHEMICAL RESPONSE TO EXPOSURE TO EXTREMELY LOW …id:PLUMEE201601V06S01A0004
  • A Comparative Analysis of the Metabolic and Coagulative Profiles in Patients with Idiopathic Scoliosis, Congenital Scoliosis and Healthy Controls: A Case …Pubmed: 30322254
  • cBmal1 but not cClock is associated with monochromatic green light-induced circadian rhythm of melatonin in chick pinealocytesPubmed: 30533004
  • Profile of melatonin and its receptors and synthesizing enzymes in cumulus–oocyte complexes of the developing sheep antral follicle—a potential estradiol …Pubmed: 30606208
  • Effect of pinealectomy on the circadian clock of the chick retina under different monochromatic lightsPubmed: 30663441
  • Plasma levels of melatonin in dilated cardiomyopathyPubmed: 30715754
  • Elevated Serum Melatonin under Constant Darkness Enhances Neural Repair in Spinal Cord Injury through Regulation of Circadian Clock Proteins ExpressionPubmed: 30678072
  • Melatonergic agonist regulates circadian clock genes and peripheral inflammatory and neuroplasticity markers in patients with depression and anxiety
  • Mel1c Mediated Monochromatic Light-Stimulated IGF-I Synthesis through the Intracellular Gαq/PKC/ERK Signaling PathwayPubmed: 30987295
  • Role of melatonin in sleep deprivation‐induced intestinal barrier dysfunction in micePubmed: 30929267
  • Melatonin mediates monochromatic green light-induced satellite cell proliferation and muscle growth in chick embryoPubmed: 31059537
  • Effect of the melatonin nuclear receptor RORα on monochromatic light-induced T-lymphocyte proliferation in chicken thymusPubmed: 31326445
  • Effect of melatonin on monochromatic light-induced changes in clock gene circadian expression in the chick liverPubmed: 31247384
  • Melatonin maintains mitochondrial membrane potential and decreases excessive intracellular Ca2+ levels in immature human oocytesPubmed: 31472147
  • Conditional Controlled Light/Dark Cycle Influences Exercise-Induced Benefits in a Rat Model with Osteoarthritis: In Vitro and In Vivo StudyPubmed: 31684092
  • Effect of monochromatic light on the circadian clock of cultured chick retinal tissuePubmed: 32198015
  • Saliva cortisol, melatonin levels and circadian rhythm alterations in Chinese primary school children with dyslexiaPubmed: 32028434
  • Role of melatonin in intestinal mucosal injury induced by restraint stress in micePubmed: 32298156
  • Hypoxia-mediated inhibition of cholesterol synthesis leads to disruption of nocturnal sex steroidogenesis in the gonad of koi carp, Cyprinus carpioPubmed: 33034795
  • Sleep deprivation aggravated lipopolysaccharide/d-galactosamine-induced acute liver injury by suppressing melatonin productionPubmed: 32809047
  • Effects of tryptophan, a precursor for melatonin, on IVF outcomes and Doppler parameters
  • 急性睡眠剥夺对小鼠血浆褪黑激素水平和下丘脑钟基因表达的影响
  • Influence of phytomelatonin on immune cell function in male golden hamsters
  • Investigation of the effect of training on serotonin, melatonin and hematologic parameters in adolescent basketball players34090322
  • Melatonin Promotes Heterotopic Ossification Through Regulation of Endothelial-Mesenchymal Transition in Injured Achilles Tendons in Rats33644068
  • A Green and Blue Monochromatic Light Combination Therapy Reduces Oxidative Stress and Enhances B-Lymphocyte Proliferation through Promoting ¡­33828639
  • Identification of Serotonin as a Predictive Marker for Breast Cancer Patients34045888
  • Physiological crosstalk between the Mel1a and Mel1c pathways modulates melatonin-mediated, monochromatic light combination-induced bursa B-lymphocyte?¡­
  • MiR-375-3p mediates reduced pineal function in hypoxia-ischemia brain damage34280452
  • Parameters of Oxidative Stress, Vitamin D, Osteopontin, and Melatonin in Patients with Lip, Oral Cavity, and Pharyngeal Cancer34721756
  • Melatonin Nuclear Receptors Mediate Green-and-Blue-Monochromatic-Light-Combinations-Inhibited B Lymphocyte Apoptosis in the Bursa of Chickens via …Pubmed:35453433

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