ELISA Kit DIY Materials for Mitochondrial Open Reading Frame Of The 12S rRNA-c (MOTS-c) 
- UOM
- FOB US$ 1,386.00 US$ 2,310.00 US$ 4,158.00 US$ 8,085.00 US$ 13,860.00
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Overview
Properties
- Product No.KSX132Hu11
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- ApplicationsMain materials for "Do It (ELISA Kit) Yourself".
Research use only - DownloadInstruction Manual
- CategoryDevelopmental science
- Reagent Contents Capture Antibody, Biotin-labeled Competitor, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
- Detectable Sampleserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- Applicable PrincipleCompetitive Inhibition ELISA for Antigen Detection
- Detectable Range4.94-400ng/mL
- Applicable Sensitivity
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ISO9001: 2008, ISO13485: 2003 Registered
Specifity
The Abs in the kit have high sensitivity and excellent specificity for detection of Mitochondrial Open Reading Frame Of The 12S rRNA-c (MOTS-c). No significant cross-reactivity or interference between Mitochondrial Open Reading Frame Of The 12S rRNA-c (MOTS-c) and analogues was observed.
Usage
1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.
Storage
Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.
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Citations
- Circulating MOTS‐c levels are decreased in obese male children and adolescents and associated with insulin resistancePubmed:29691953
- Development of a mass spectrometry‐based detection method for the mitochondrion‐derived peptide MOTS‐c in plasma samples for doping control purposesPubmed: 30394592
- Adiponectin treatment improves insulin resistance in mice by regulating the expression of the mitochondrial-derived peptide MOTS-c and its response to exercise via …Pubmed: 32880686
- Induction of hyperandrogenism and insulin resistance differentially modulates ferroptosis in uterine and placental tissues of pregnant rats
- Neural cell©\derived plasma exosome protein abnormalities implicate mitochondrial impairment in first episodes of psychosis33454965
- MOTS-c interacts synergistically with exercise intervention to regulate PGC-1¦Á expression, attenuate insulin resistance and enhance glucose metabolism in mice via?¡33722744
- Evolution of Mitochondrial-Derived Peptides, Humanin and MOTSc and Changes in Insulin Sensitivity During Early Gestation
- Abnormal levels of mitochondrial proteins in plasma neuronal extracellular vesicles in major depressive disorder34471251
- The Mitochondrial-Derived Peptide MOTS-c Relieves Hyperglycemia and Insulin Resistance in Gestational Diabetes Mellitus34798268
- SARS‐CoV‐2 and mitochondrial proteins in neural‐derived exosomes of COVID‐19Pubmed:35285072
- Evolution of Mitochondrially Derived Peptides Humanin and MOTSc, and Changes in Insulin Sensitivity during Early Gestation in Women with and without Gestational …Pubmed:35683389