ELISA Kit DIY Materials for Motilin (MTL) Homo sapiens (Human) Assay Kit DIY Materials

MLN; MAP; Promotilin; Motilin-associated peptide

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Overview
Properties
  • Reagent Contents Capture Antibody, Biotin-labeled Competitor, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable SampleSerum, plasma and other biological fluids.
  • Applicable PrincipleCompetitive Inhibition ELISA for Antigen Detection
  • Detectable Range74.07-6,000pg/mL
  • Applicable Sensitivity27.38pg/mL
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  • ELISA Kit DIY Materials for Motilin (MTL) Packages (Simulation)
  • ELISA Kit DIY Materials for Motilin (MTL) Packages (Simulation)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The Abs in the kit have high sensitivity and excellent specificity for detection of Motilin (MTL). No significant cross-reactivity or interference between Motilin (MTL) and analogues was observed.

Usage

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

Storage

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

Support pack

Citations

  • 清醒状态下热射病大鼠胃肠动力与胃泌素、胃动素的变化Wjgnet:Source
  • Using the "target constituent removal combined with bioactivity assay" strategy to investigate the optimum arecoline content in charred areca nut.pubmed:28054652
  • Positive enhancement of Lactobacillus fermentum HY01 on intestinal movements of mice having constipation10.1007/s13765-017-0327-3
  • Colonic Transit Time and Gut Peptides in Adult Patients with Slow and Normal Colonic Transit Constipationpubmed:29082242
  • Home-Based Transcutaneous Neuromodulation Improved Constipation via Modulating Gastrointestinal Hormones and Bile AcidsPubmed:29853946
  • The Relations Among Serum Ghrelin, Motilin and Gastric Emptying and Autonomic Function in Autoimmune GastritisPubmed:29753372
  • Integrative effects of transcutaneous electrical acustimulation on abdominal pain, gastrointestinal motility, and inflammation in patients with early‐stage acute …34536258
  • The Inhibitory Effects of Naringin in a Rat Model of Postoperative Intraperitoneal Adhesion FormationPubmed:35069760

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