ELISA Kit DIY Materials for Myelin Associated Glycoprotein (MAG) Homo sapiens (Human) Assay Kit DIY Materials

GMA; S-MAG; Siglec-4; SIGLEC4A; Sialic Acid Binding Ig-Like Lectin 4A

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Overview
Properties
  • Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable Sampletissue homogenates, cell lysates, cell culture supernates and other biological fluids
  • Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
  • Detectable Range0.312-20ng/mL
  • Applicable Sensitivity0.112ng/mL
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  • ELISA Kit DIY Materials for Myelin Associated Glycoprotein (MAG) Packages (Simulation)
  • ELISA Kit DIY Materials for Myelin Associated Glycoprotein (MAG) Packages (Simulation)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The Abs in the kit have high sensitivity and excellent specificity for detection of Myelin Associated Glycoprotein (MAG). No significant cross-reactivity or interference between Myelin Associated Glycoprotein (MAG) and analogues was observed.

Usage

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

Storage

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

Support pack

Citations

  • Effect of bimodal pore structure on the bioactivity of poly(lactic-co-glycolic acid)/poly(γ-glutamic acid)/Pluronic 17R4 nerve conduitss10853-016-0729-3
  • Effect of bimodal pore structure on the bioactivity of poly (lactic-co-glycolic acid)/poly (γ-glutamic acid)/Pluronic 17R4 nerve conduitsNRF-2015K2A2A4000128

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