ELISA Kit DIY Materials for Myoglobin (MYO) Rattus norvegicus (Rat) Assay Kit DIY Materials

PVALB; MB

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Overview
Properties
  • Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable SampleSerum, plasma, tissue homogenates and other biological fluids.
  • Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
  • Detectable Range0.625-40ng/mL
  • Applicable Sensitivity0.238ng/mL
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  • ELISA Kit DIY Materials for Myoglobin (MYO) Packages (Simulation)
  • ELISA Kit DIY Materials for Myoglobin (MYO) Packages (Simulation)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The Abs in the kit have high sensitivity and excellent specificity for detection of Myoglobin (MYO). No significant cross-reactivity or interference between Myoglobin (MYO) and analogues was observed.

Usage

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

Storage

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

Support pack

Citations

  • Acute leukocyte, cytokine and adipocytokine responses to maximal and hypertrophic resistance exercise boutsPubmed:25145982
  • Transcriptional Alterations by Ischaemic Postconditioning in a Pig Infarction Model: Impact on Microvascular ProtectionPubmed: 30650650
  • Electrochemical dual-aptamer-based biosensor for nonenzymatic detection of cardiac troponin I by nanohybrid electrocatalysts labeling combined with DNA …Pubmed: 30954926
  • DNA nanotetrahedron linked dual-aptamer based voltammetric aptasensor for cardiac troponin I using a magnetic metal-organic framework as a labelPubmed: 31123904
  • Emitter–Quencher Pair of Single Atomic Co Sites and Monolayer Titanium Carbide MXenes for Luminol Chemiluminescent Reactions34914377
  • A rapid fluorescent aptasensor for point-of-care detection of C-reactive proteinPubmed:35714415

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