ELISA Kit DIY Materials for Nesfatin 1 (NES1) Homo sapiens (Human) Assay Kit DIY Materials

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Overview
Properties
  • Reagent Contents Capture Antibody, Biotin-labeled Competitor, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable SampleSerum, plasma and other biological fluids.
  • Applicable PrincipleCompetitive Inhibition ELISA for Antigen Detection
  • Detectable Range617.3-50000pg/mL
  • Applicable Sensitivity251.4pg/mL
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  • ELISA Kit DIY Materials for Nesfatin 1 (NES1) Packages (Simulation)
  • ELISA Kit DIY Materials for Nesfatin 1 (NES1) Packages (Simulation)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The Abs in the kit have high sensitivity and excellent specificity for detection of Nesfatin 1 (NES1). No significant cross-reactivity or interference between Nesfatin 1 (NES1) and analogues was observed.

Usage

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

Storage

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

Support pack

Citations

  • Plasma Nesfatin-1 Level May Be Associated with Disease Severity in Patients with Panic DisorderPsikofarmakoloji: 2043
  • High plasma nesfatin-1 level in patients with major depressive disorderPubMed: 21163317
  • Cord blood nesfatin-1 and apelin-36 levels in gestational diabetes mellitusSpringerLink: h978088657555w16
  • Increased Plasma Nesfatin-1 Levels in Patients with Obsessive Compulsive Disorder Psikofarmakoloji: 2212
  • Decreased plasma nesfatin-1 levels in patients with generalized anxiety disorderPubMed: 22595767
  • Effects of Aerobic Training, with or without Zizyphus Jujuba Water Extraction, on Fundus Nesfatin-1, ATP, HDL-C, and LDL-C Concentrations in Female RatsIjvst: Source
  • Decreased serum nesfatin-1 levels in endometriosisPubmed: 24702902
  • Increased Plasma Nesfatin-1 Levels in Patients with Obsessive Compulsie Disorder.Psikofarmakoloji: Source
  • Role of NUCB2/nesfatin-1 as a possible biomarker.Pubmed: 23537082
  • A role of nesfatin-1/NucB2 in dehydration-induced anorexiaPubmed:24829503
  • Plasma nesfatin-1 levels are increased in patients with polycystic ovary syndromePubmed:24920281
  • The potential role of BMI, plasma leptin, nesfatin-1 and ghrelin levels in the early detection of pancreatic necrosis and severe acute pancreatitis: a prospective cohort study.Pubmed:25448651
  • The effects of short-term high-intensity interval training vs. moderate-intensity continuous training on plasma levels of nesfatin-1 and inflammatory markersPubmed:25581765
  • New hormones to predict the severity of gallstone-induced acute pancreatitisPubmed:25599787
  • Is There a Relationship between Obstructive Sleep Apnea Syndrome Severity and Nesfatin-1?PubMed: 26088934
  • Correlation of Brain Neuropeptide (Nesfatin-1 and Orexin-A) Concentrations with Anthropometric and Biochemical Parameters in Malnourished ChildrenPubMed: 26831553
  • Nesfatin-1 and Vitamin D levels may be associated with systolic and diastolic blood pressure values and hearth rate in polycystic ovary syndromePubMed: 26295295
  • Relationship and Interaction between Anaerobic Sports Branches and Serum Nesfatin-1Sayilar: 294
  • Nesfatin-1 levels and metabolic markers in polycystic ovary syndromePubMed: 26062107
  • Serum nesfatin-1 levels: a potential new biomarker in patients with subarachnoid hemorrhage*Pubmed:26872406
  • Comparing the nesfatin-1 levels between individuals with subjective cognitive decline and mild cognitive impairmentP.1.e.012.pdf
  • Serum nesfatin-1 levels: a potential new biomarker in patients with subarachnoid hemorrhage.pubmed:26872406
  • Metabolic and Cardiovascular Actions of Nesfatin-1: Implications in Health and Diseasepubmed:28137218
  • Serum nesfatin-1 levels are decreased in pregnant women newly diagnosed with gestational diabetespubmed:28977160
  • Serum NUCB2/nesfatin-1 levels in different stages of alcohol dependence: Is there a relationship with craving?pubmed:28529367
  • Nesfatin-1 promotes VSMC migration and neointimal hyperplasia by upregulating matrix metalloproteinases and downregulating PPARγPubmed:29604590
  • Nesfatin-1 functions as a switch for phenotype transformation and proliferation of VSMCs in hypertensive vascular remodelingPubmed:29627363
  • Plasma nesfatin-1 level is associated with severity of depression in Chinese depressive patientsPubmed:29615007
  • Mitra Zarrati, Mahsa Raji Lahiji, Eisa Salehi, Bahareh Yazdani, Elham Razmpoosh, Raheleh Shokouhi Shoormasti & Farzad ShidfarPubmed: 30232744
  • An elevated serum level of nesfatin-1 may predict poor outcomes in patients with aneurysmal subarachnoid hemorrhages
  • Relationshıp of spontaneous subarachnoid haemorrhage and cerebral aneurysm to serum Visfatin and Nesfatin-1 levelsPubmed: 32311618
  • Copeptin and Nesfatin-1 Are Interrelated Biomarkers with Roles in the Pathogenesis of Insulin Resistance in Chinese Children with ObesityPubmed: 33027789
  • Assessing the blood concentration of new adipocytokines in patients with ischaemic stroke33283259
  • Increased serum nesfatin-1 levels among adolescents diagnosed with major depressive disorder

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