ELISA Kit DIY Materials for Neuregulin 1 (NRG1) Homo sapiens (Human) Assay Kit DIY Materials

ARIA; GGF; HGL; HRG; HRGA; NDF; SMDF; Acetylcholine receptor-inducing activity; Glial growth factor; Heregulin; Neu differentiation factor; Sensory and motor neuron-derived factor

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Overview
Properties
  • Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable SampleTissue homogenates, cell lysates, cell culture supernates and other biological fluids
  • Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
  • Detectable Range0.156-10ng/mL
  • Applicable Sensitivity0.055ng/mL
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  • ELISA Kit DIY Materials for Neuregulin 1 (NRG1) Packages (Simulation)
  • ELISA Kit DIY Materials for Neuregulin 1 (NRG1) Packages (Simulation)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The Abs in the kit have high sensitivity and excellent specificity for detection of  Neuregulin 1 (NRG1). No significant cross-reactivity or interference between  Neuregulin 1 (NRG1) and analogues was observed.

Usage

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

Storage

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

Support pack

Citations

  • Therapeutic effect of co-transplantation of neuregulin 1-transfected-Schwann cells and bone marrow stromal cells on spinal cord hemisection syndromCyagen: source
  • ADAM17 Silencing in Mouse Colon Carcinoma Cells: The Effect on Tumoricidal Cytokines and AngiogenesisPubMed: PMC3519469
  • Cerebrospinal fluid–based kinetic biomarkers of axonal transport in monitoring neurodegenerationPubMed: PMC3428100
  • Dysregulation of Neuregulin-1/ErbB signaling in the prefrontal cortex and hippocampus of rats exposed to chronic unpredictable mild stressPubMed: 26626816
  • Effects of prolonged antipsychotic administration on neureCavia (Guinea pig )lin-1/ErbB signaling in rat prefrontal cortex and myocardium: implications for the therapeutic action and cardiac adverse effect.Pubmed:26961615
  • Qiliqiangxin protects against anoxic injury in cardiac microvascular endothelial cells via NRG-1/ErbB-PI3K/Akt/mTOR pathway.pubmed:28271613
  • Ablation of neuropsin-neuregulin 1 signaling imbalances ErbB4 inhibitory networks and disrupts hippocampal gamma oscillation.pubmed:28267150
  • A proteasome-resistant fragment of NIK mediates oncogenic NF-κB signaling in schwannomasPubmed: 30335132
  • Jinmaitong ameliorates diabetic peripheral neuropathy in streptozotocin-induced diabetic rats by modulating gut microbiota and neuregulinPubmed: 32920546

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