ELISA Kit DIY Materials for Neurofilament, Light Polypeptide (NEFL)
CMT1F; CMT2E; NF-L; NF68; NFL; 68 kDa neurofilament protein; Neurofilament triplet L protein
- UOM
- FOB US$ 1,260.00 US$ 2,100.00 US$ 3,780.00 US$ 7,350.00 US$ 12,600.00
- Quantity
Overview
Properties
- Product No.KSE038Hu01
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- ApplicationsMain materials for "Do It (ELISA Kit) Yourself".
Research use only - DownloadInstruction Manual
- CategoryNeuro science
- Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
- Detectable SampleSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
- Detectable Range0.156-10ng/mL
- Applicable Sensitivity0.057ng/mL
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Specifity
The Abs in the kit have high sensitivity and excellent specificity for detection of Neurofilament, Light Polypeptide (NEFL). No significant cross-reactivity or interference between Neurofilament, Light Polypeptide (NEFL) and analogues was observed.
Usage
1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.
Storage
Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.
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Citations
- Effect of carotid endarterectomy on brain damage markersPubmed:27126899
- Effect of carotid endarterectomy on brain damage markers.pubmed:27126899
- Cytokines and biological markers in autoimmune GFAP astrocytopathy: The potential role for pathogenesis and therapeutic implicationsPubmed: 31254929
- Neurofilament Light Chain Is a Biomarker of Neurodegeneration in Ataxia Telangiectasia33893614
- Phase 1b dose-escalation, safety, and pharmacokinetic study of IC14, a monoclonal antibody against CD14, for the treatment of amyotrophic lateral sclerosis34678870
- Fatty Acid-Binding Protein 7 (FABP-7), Glutamic Acid and Neurofilament Light Chain (NFL) as Potential Markers of Neurodegenerative Disorders in Psoriatic Patients …Pubmed:35566558