ELISA Kit DIY Materials for Neuropeptide Y (NPY) Homo sapiens (Human) Assay Kit DIY Materials

NP-Y; PYY4; CPON; Pro-neuropeptide Y; Neuropeptide tyrosine; C-flanking peptide of NPY

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Overview
Properties
  • Reagent Contents Capture Antibody, Biotin-labeled Competitor, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable SampleSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
  • Applicable PrincipleCompetitive Inhibition ELISA for Antigen Detection
  • Detectable Range24.69-2000pg/mL
  • Applicable Sensitivity9.18pg/mL
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  • ELISA Kit DIY Materials for Neuropeptide Y (NPY) Packages (Simulation)
  • ELISA Kit DIY Materials for Neuropeptide Y (NPY) Packages (Simulation)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The Abs in the kit have high sensitivity and excellent specificity for detection of Neuropeptide Y (NPY). No significant cross-reactivity or interference between Neuropeptide Y (NPY) and analogues was observed.

Usage

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

Storage

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

Support pack

Citations

  • Is there any interaction of resistin and adiponectin levels with protein‐energy wasting among patients with chronic kidney diseaseOnlinelibrary: hdi.12072
  • Changes in growth pattern, leptin ghrelin and neuropeptide Y levels after adenotonsillectomy in prepubertal childrenDegruyter: Source
  • The role of adipocytokines on depressive symptoms of patients with chronic kidney diseasePubmed: 23879728
  • The relationship of plasma neuropeptide Y levels with coronary collateral development.Pubmed: 24077326
  • The relationship of plasma neuropeptide Y levels with coronary collateral developmentLww:Source
  • Possible role of serotonin and neuropeptide Y on the disruption of the reproductive axis activity by perfluorooctane sulfonatePubmed:25623392
  • High Plasma Levels of Neuropeptide Y Correlate With Good Clinical Outcome But are not Correlated to Cerebral Blood Flow or Vasospasm After Subarachnoid HemorrhagePubMed: 25955865
  • High Intensity Aerobic Exercise Training Improves Deficits of Cardiovascular Autonomic Function in a Rat Model of Type 1 Diabetes Mellitus with Moderate …Pubmed:26885531
  • Binge eating and biochemical markers of appetite in new users of the contraceptive depot medroxyprogesterone acetatepubmed:27604241
  • Effects of Di-isononyl Phthalate on Neuropeptide Y Expression in Differentiating Human Neuronal Cells.pubmed:27625336
  • Aberrant changes of somatostatin and neuropeptide Y in brain of a genetic rat model for epilepsy: tremor ratpubmed:27685769
  • Nur77 protects against adverse cardiac remodelling by limiting Neuropeptide Y signalling in the sympathoadrenal-cardiac axisPubmed:29850786
  • Salivary neuropeptides, stress, and periodontitisPubmed:28914594
  • Mechanisms of sustained long-term weight loss after RYGB: α-MSH is a key factorPubmed:29685637
  • Melatonin Induces Osteoblastic Differentiation of Mesenchymal Stem Cells and Promotes Fracture Healing in a Rat Model of Femoral Fracture via Neuropeptide Y …Pubmed: 30227410
  • Investigation of the relationship between insulin resistance and neuropeptide Y levels in polycystic ovary syndrome
  • Investigation of the relationship between insulin resistance and neuropeptide Y levels in polycystic ovary syndrome.
  • Sevoflurane Induces Hippocampal Neuronal Apoptosis by Altering the Level of Neuropeptide Y in Neonatal Rats.Pubmed: 32378074
  • Serum levels of neuropeptide Y in patients with chronic schizophrenia during treatment augmentation with sarcosine (results of the double©\blind randomized?¡­33245168
  • Serotonin involvement in okadaic acid-induced diarrhoea in vivo34148100

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