ELISA Kit DIY Materials for Neurotensin (NT)
NTS; NTS1; NmN-125; NN; Tail peptide Neuromedin N
- UOM
- FOB US$ 1,300.00 US$ 2,166.00 US$ 3,899.00 US$ 7,581.00 US$ 12,996.00
- Quantity
Overview
Properties
- Product No.KSB203Ra11
- Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
- ApplicationsMain materials for "Do It (ELISA Kit) Yourself".
Research use only - Downloadn/a
- CategoryReproductive scienceNeuro scienceHormone metabolism
- Reagent Contents Capture Antibody, Biotin-labeled Competitor, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
- Detectable SampleSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
- Applicable PrincipleCompetitive Inhibition ELISA for Antigen Detection
- Detectable Range37.04-3000pg/mL
- Applicable Sensitivity15.2pg/mL
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Specifity
The Abs in the kit have high sensitivity and excellent specificity for detection of Neurotensin (NT). No significant cross-reactivity or interference between Neurotensin (NT) and analogues was observed.
Usage
1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.
Storage
Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.
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Citations
- Colonic Transit Time and Gut Peptides in Adult Patients with Slow and Normal Colonic Transit Constipationpubmed:29082242
- Inhibition of Peroxynitrite-induced mitophagy activation attenuates cerebral ischemia-reperfusion injuryPubmed:29307080
- Adipose Tissue-Derived Biomarkers of Intestinal Barrier Functions for the Characterization of Diarrhoea-Predominant IBS
- Functional analysis of gut microbiota and immunoinflammation in children with autism spectrum disordersPubmed: 31320306
- Association between Circular RNA CDR1as and Post-Infarction Cardiac Function in Pig Ischemic Heart Failure: Influence of the Anti-Fibrotic Natural Compounds …Pubmed: 32823854
- Qiliqiangxin Improves Cardiac Function through Regulating Energy Metabolism via HIF-1α-Dependent and Independent Mechanisms in Heart Failure Rats …Pubmed: 32626732
- Identification of U-shaped Curve Relation in Pro-neurotensin and Risk of Coronary Artery Disease (CAD) in Premature CAD Patients
- Effect of dobutamine on intrinsic myocardial function and myocardial apoptosis in septic rats with myocardial dysfunction
- Harmine is an effective therapeutic small molecule for the treatment of cardiac hypertrophy33785860
- Elabela gene therapy promotes angiogenesis after myocardial infarction34291565
- Renal Impairment Detectors: IGFBP-7 and NGAL as Tubular Injury Markers in Multiple Myeloma Patients34946293