ELISA Kit DIY Materials for Nuclear Factor Kappa B (NFkB)
NF-KB1; EBP-1; KBF1; NF-Kappa-B; NFKB-P105; NFKB-P50; Nuclear Factor Of Kappa Light Polypeptide Gene Enhancer In B-Cells 1; Nuclear factor NF-kappa-B p105 subunit
- UOM
- FOB US$ 1,300.00 US$ 2,166.00 US$ 3,899.00 US$ 7,581.00 US$ 12,996.00
- Quantity
Overview
Properties
- Product No.KSB824Ra01
- Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
- ApplicationsMain materials for "Do It (ELISA Kit) Yourself".
Research use only - Downloadn/a
- CategorySignal transduction
- Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
- Detectable SampleTissue homogenates, cell lysates, cell culture supernates and other biological fluids.
- Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
- Detectable Range0.312-20ng/mL
- Applicable Sensitivity0.114ng/mL
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Specifity
The Abs in the kit have high sensitivity and excellent specificity for detection of Nuclear Factor Kappa B (NFkB). No significant cross-reactivity or interference between Nuclear Factor Kappa B (NFkB) and analogues was observed.
Usage
1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.
Storage
Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.
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Citations
- Surgical Removal of Endometrioma Decreases the NF-kB1 (p50/105) and NF-kB p65 (Rel A) Expression in the Eutopic Endometrium During the Implantation WindowSagepub: Source
- Proanthocyanidins from grape seeds modulates the nuclear factor-kappa B signal transduction pathways in rats with TNBS-induced recurrent ulcerative colitisPubMed: 21642017
- New Method to Isolate and Culture Rat Kupffer CellsPubMed: PMC3743898
- Long-term aspirin pretreatment in the prevention of cerulein-induced acute pancreatitis in ratsPubMed: PMC3660814
- Shuangtengbitong tincture treatment of collagen-induced arthritis via downregulation of the expression of IL-6, IL-8, TNF-α and NF-κBPubMed: PMC3570159
- Cilostazol Renoprotective Effect: Modulation of PPAR-γ, NGAL, KIM-1 and IL-18 Underlies Its Novel Effect in a Model of Ischemia-ReperfusionPubmed:24816434
- Involvement of nitric oxide with activation of Toll-like receptor 4 signaling in mice with dextran sodium sulfate-induced colitisPubmed:24992835
- Long-term warfarin therapy and biomarkers for osteoporosis and atherosclerosisPubMed: 26674156
- Serological Analysis of Alpha-synuclein and NF-κB in Parkinson’s Disease PatientsPubMed: 26155470
- Hysteroscopic polypectomy decreases NF-κB1 expression in the mid-secretory endometrium of women with endometrial polypPubMed: 25898371
- Plasma nuclear factor kappa B and serum peroxiredoxin 3 in early diagnosis of hepatocellular carcinoma.PubMed: 25743848
- Preventive use of Lactobacillus plantarum LS/07 and inulin to relieve symptoms of acute colitis.PubMed: 26345092
- Use Of The Prebiotic Inulin In The Prevention Of Adverse Signs Of Acute ColitisView: J
- Protective Effect of Oral Hesperetin Against Unilateral Striatal 6-Hydroxydopamine Damage in the RatPubMed: 26700436
- L-Alanyl-Glutamine Attenuates Oxidative Stress in Liver Transplantation PatientsPubMed: 26518955
- Effects of Hyriopsis cumingii Polysaccharides on Mice Immunologic Receptor, Transcription Factor, and CytokinePubmed:27061742
- THE PROPHYLACTIC EFFICIENCY OF LYCOPENE AGAINST GAMMA IRRADAITION-INDIUCED CARDIAC OXIDATIVE DAMAGE, PATHOLOGY AND APOPTOSIS IN RATSpublication:298838934
- Placental Growth Factor Triggers Epithelial-to-Mesenchymal Transition-like Changes in Rat Type II Alveolar Epithelial Cells: Activation of Nuclear Factor κB Signalling Pathwaydoi:10.1111
- Carnosine ameliorates cognitive deficits in streptozotocin-induced diabetic rats: Possible involved mechanisms.pubmed:27777064
- Forskolin, a hedgehog signalling inhibitor, attenuates carbon tetrachloride-induced liver fibrosis in rats.pubmed:27590029
- Garlic active constituent s-allyl cysteine protects against lipopolysaccharide-induced cognitive deficits in the rat: Possible involved mechanisms.pubmed:27915041
- S-allyl cysteine ameliorates cognitive deficits in streptozotocin-diabetic rats via suppression of oxidative stress, inflammation, and acetylcholinesterase.pubmed:27887948
- Ellagic acid ameliorates learning and memory deficits in a rat model of Alzheimer's disease: an exploration of underlying mechanisms.pubmed:28303372
- Acetyl-l-carnitine protects dopaminergic nigrostriatal pathway in 6-hydroxydopamine-induced model of Parkinson's disease in the rat.pubmed:28199883
- Diosgenin ameliorates development of neuropathic pain in diabetic rats: Involvement of oxidative stress and inflammation.pubmed:28033582
- Sildenafil protects against bile duct ligation induced hepaticfibrosis in rats: Potential role for silent information regulator 1 (SIRT1)pubmed:28974454
- Immunomodulatory Activities of a Fungal Protein Extracted from Hericium erinaceus through Regulating the Gut Microbiotapubmed:28713364
- The shear wave elastic modulus and the increased nuclear factor kappa B(NF-kB/p65) and cyclooxygenase-2 (COX-2) expression in the area of myofascial trigger points activated in a rat model by blunt trauma to the vastus medialispubmed:29137729
- In vivo cellular and molecular gastroprotective mechanisms of chrysin; Emphasis on oxidative stress, inflammation and angiogenesispubmed:29126792
- Docking Studies and Biological Evaluation of a Potential β-Secretase Inhibitor of 3-Hydroxyhericenone F from Hericium erinaceus.pubmed:28553224
- Anti-Inflammatory Activity and Bioactive Constituents of Cultivated Fruiting Bodies of Xylaria nigripes (Ascomycetes), a Chinese Medicinal Fungus.pubmed:29256845
- Berberine ameliorates intrahippocampal kainate-induced status epilepticus and consequent epileptogenic process in the rat: Underlying mechanismspubmed:28061403
- Scutellarin alleviates lipopolysaccharide-induced cognitive deficits in the rat: Insights into underlying mechanisms.pubmed:29190543
- Antioxidant, anti-inflammatory and synergistic anti-hyperglycemic effects of Malaysian propolis and metformin in streptozotocin–induced diabetic ratsPubmed:30026088
- Protective Effects of Fibroblast Growth Factor 21 Against Amyloid-Beta-Induced Toxicity in SH-SY5Y CellsPubmed:29869772
- Naringenin ameliorates learning and memory impairment following systemic lipopolysaccharide challenge in the ratPubmed:29518393
- Shock waves increase pulmonary vascular leakage, inflammation, oxidative stress, and apoptosis in a mouse modelPubmed:29984607
- Anti-inflammatory potential of Lactobacillus plantarum LS/07 in acute colitis in rats10.2478:acve-2018-0005
- HMGB1-RAGE signaling pathway in pPROMPubmed:29673663
- Improvement of colitis by LS/07 and inulin10.2754:avb201786040399
- The potential antifibrotic impact of apocynin and alpha-lipoic acid in concanavalin A-induced liver fibrosis in rats: Role of NADPH oxidases 1 and 4Pubmed:29760339
- Malaysian propolis, metformin and their combination, exert hepatoprotective effect in streptozotocin-induced diabetic ratsPubmed: 30205096
- Sitagliptin attenuates intestinal ischemia/reperfusion injury via cAMP/PKA, PI3K/Akt pathway in a glucagon-like peptide 1 receptor-dependent mannerPubmed: 30195035
- Effect of Dachaihu Decoction on non-alcoholic fatty liver disease model rats induced by a high-fat high-sugar diet
- Amitriptyline attenuates bleomycin-induced pulmonary fibrosis: modulation of the expression of NF-κβ, iNOS, and Nrf2Pubmed: 30474696
- Diabetes-induced testicular oxidative stress, inflammation, and caspase-dependent apoptosis: the protective role of metforminPubmed: 30513216
- Complex interaction of dietary fat and Alaskan bog blueberry supplementation influences manganese mediated neurotoxicity and behavioral impairments
- Anti-breast Cancer Activity of SPG-56 from Sweet Potato in MCF-7 Bearing Mice in Situ through Promoting Apoptosis and Inhibiting MetastasisPubmed: 30651572
- Increased cerebral nuclear factor kappa B in a complex regional pain syndrome rat model: possible relationship between peripheral injury and the brainPubmed: 30881100
- Oxidative Stress, NF-κB-Mediated Inflammation and Apoptosis in the Testes of Streptozotocin–Induced Diabetic Rats: Combined Protective Effects of Malaysian …Pubmed: 31600920
- Indomethacin and juglone inhibit inflammatory molecules to induce apoptosis in colon cancer cellsPubmed: 31916655
- New insights into the protection of growth hormone in cisplatin-induced nephrotoxicity: The impact of IGF-1 on the Keap1-Nrf2/HO-1 signalingPubmed: 32209424
- Effects of lncRNA gm4419 on rats with hypertensive cerebral atherosclerosis through NF-κB pathwayPubmed: 31858567
- Effects of fermented ginseng on the gut microbiota and immunity of rats with antibiotic-associated diarrheaPubmed: 33217518
- Effects of different pulp-capping materials on cell death signaling pathways of lipoteichoic acid-stimulated human dental pulp stem cellsPubmed: 33206337
- Effects of combined treatment with Indomethacin and Juglone on AOM/DSS induced colon carcinogenesis in Balb/c mice: Roles of inflammation and …Pubmed: 33148421
- Morroniside attenuates apoptosis and pyroptosis of chondrocytes and ameliorates osteoarthritic development by inhibiting NF-κB signalingPubmed: 33022338
- Examination of androgenetic alopecia with serum biomarkersPubmed: 32969583
- Diosgenin Attenuates Cognitive Impairment in Streptozotocin-Induced Diabetic Rats: Underlying MechanismsPubmed: 32526752
- UPLC-Orbitrap HRMS metabolic profiling of Cymbopogon citratus cultivated in Egypt; neuroprotective effect against AlCl3-induced neurotoxicity in ratsPubmed: 32376365
- Protective effects of bee bread on testicular oxidative stress, NF-κB-mediated inflammation, apoptosis and lactate transport decline in obese male ratsPubmed: 33152939
- Regulation and Mechanism of miR-518d through the PPARα-Mediated NF-κB Pathway in the Development of Gestational Diabetes MellitusPubmed: 33123597
- Effect of Graviola (Annona Muricata l.) and Ginger (Zingiber Officinale Roscoe) on Diabetes Mellitus Induced in Male Wistar Albino Rats
- Cyproheptadine causes apoptosis and decreases inflammation by disrupting thiol/disulfide balance and enhancing the levels of SIRT1 in C6 glioblastoma cells33675893
- Alamandine significantly reduces doxorubicin-induced cardiotoxicity in rats33882726
- Masseter muscle and gingival tissue inflammatory response following treatment with high©\fructose corn syrup in rats: Anti©\inflammatory and antioxidant effects of kefir33864286
- The Potential of Nano Curcumin in Preventing the Formation of Artificial Antisperm Antibody in Wistar Rats through Inflammatory Pathway Regulation
- Vitamin D supplementation inhibits NF-kß signaling pathway in lean and obese women with PCOSPubmed:35731068
- Effect of subacute poisoning with lambdacyhalothrin on vascular endothelial growth factor 2 receptor in mice kidneysPubmed:35643467
- Therapeutic Effects of Bee Bread on Obesity-Induced Testicular-Derived Oxidative Stress, Inflammation, and Apoptosis in High-Fat Diet Obese Rat Model …Pubmed:35204140
- Concomitant Administration of Rosuvastatin and Lefleunamide in Low doses Synergize Against Complete Freund's Adjuvant (CFA)-Induced Rheumatoid …