ELISA Kit DIY Materials for Oxytocin (OT) Pan-species (General) Assay Kit DIY Materials

Pitocin; Syntocinon

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Overview
Properties
  • Reagent Contents Capture Antibody, Biotin-labeled Competitor, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable Sampleserum, plasma and other biological fluids
  • Applicable PrincipleCompetitive Inhibition ELISA for Antigen Detection
  • Detectable Range12.35-1000pg/mL
  • Applicable Sensitivity5.27pg/mL
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  • ELISA Kit DIY Materials for Oxytocin (OT) Packages (Simulation)
  • ELISA Kit DIY Materials for Oxytocin (OT) Packages (Simulation)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The Abs in the kit have high sensitivity and excellent specificity for detection of Oxytocin (OT). No significant cross-reactivity or interference between Oxytocin (OT) and analogues was observed.

Usage

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

Storage

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

Support pack

Citations

  • Sex-specific associations between plasma oxytocin levels and schizotypal personality features in healthy individualsPubmed:24411593
  • Administration of atosiban in patients with endometriosis undergoing frozen–thawed embryo transfer: a prospective, randomized studyPubmed:27143518
  • Increased oxytocin levels among abstinent heroin addicts: Association with aggressiveness, psychiatric symptoms and perceived childhood neglect.pubmed:28093220
  • Can hypoxia enhance sexual arousal?-Molecular-biological analysis of the hypothalamus in male rats placed with oestrous female rats under hypoxic …files:ijcem0031358.pdf
  • Administration of atosiban in patients with endometriosis undergoing frozen–thawed embryo transfer: a prospective, randomized studypubmed:27143518
  • Intranasal oxytocin reduces provoked symptoms in female patients with posttraumatic stress disorder despite exerting sympathomimetic and positive chronotropic effects in a randomized controlled trial.pubmed:28209155
  • Plasma oxytocin levels in major depressive and bipolar II disorderspubmed:28865715
  • Intranasal oxytocin reduces provoked symptoms in female patients with posttraumatic stress disorder despite exerting sympathomimetic and positive chronotropic effects in a randomized controlled trial10.1186:s12916-017-0801-0
  • The Association between the Level of Plasma Oxytocin and Craving among Former Heroin UsersPubmed:29902803
  • Involvement of Hyperpolarization-Activated Cyclic Nucleotide-Gated Channel 3 in Oxytocin Neuronal Activity in Lactating Rats With Pup DeprivationPubmed: 32962418
  • Effects of transcutaneous electrical nerve stimulation applied at different frequencies during labor on hormone levels, labor pain perception, and anxiety: a randomized …
  • Predicting the Treatment Outcomes of Antidepressants Using a Deep Neural Network of Deep Learning in Drug-Naïve Major Depressive Patients

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