ELISA Kit DIY Materials for Parathyroid Hormone (PTH)
iPTH; Intact Parathyroid Hormone; Parathormone; Parathyrin
- UOM
- FOB US$ 1,134.00 US$ 1,890.00 US$ 3,402.00 US$ 6,615.00 US$ 11,340.00
- Quantity
Overview
Properties
- Product No.KSA866Hu11
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- ApplicationsMain materials for "Do It (ELISA Kit) Yourself".
Research use only - DownloadInstruction Manual
- CategoryMetabolic pathwayEndocrinologyHormone metabolism
- Reagent Contents Capture Antibody, Biotin-labeled Competitor, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
- Detectable SampleSerum, plasma and other biological fluids.
- Applicable PrincipleCompetitive Inhibition ELISA for Antigen Detection
- Detectable Range2.47-200pg/mL
- Applicable Sensitivity0.91pg/mL
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Specifity
The Abs in the kit have high sensitivity and excellent specificity for detection of Parathyroid Hormone (PTH). No significant cross-reactivity or interference between Parathyroid Hormone (PTH) and analogues was observed.
Usage
1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.
Storage
Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.
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Citations
- Elevation of PTH and PTHrp Induced by Excessive Fluoride in Rats on a Calcium-deficient DietPubMed: 19915804
- Effects of duodenal redox status on calcium absorption and related genes expression in high-fat diet–fed miceScienceDirect: S0899900709004808
- Effects of a Low Calcium Diet and Oxalate Intake on Calcium Deposits in Soft Tissues and Bone Metabolism in Ovariectomized RatsKoreaMed: source
- Whole body vibration is a safe exercise training method and induces no impaired alterations on rat plasma parametersPubmed: 22173025
- The effect of supplementation of calcium, vitamin D, boron, and increased fluoride intake on bone mechanical properties and metabolic hormones in rat PubMed: 22782709
- Effect of consumption of fatty acids, calcium, vitamin D and boron with regular physical activity on bone mechanical properties and corresponding metabolic hormones in rats.PubMed: 22439438
- Is Gastrectomy-Induced High Turnover of Bone with Hyperosteoidosis and Increase of Mineralization a Typical Osteomalacia?PubMed: PMC3679169
- Effect of the “protein diet” and bone tissue.Pubmed: 24483972
- Zoledronic acid has differential anti-tumour activity in the pre-and post-menopausal bone microenvironment in vivoAacrjournals: Source
- Effect of the “protein diet” and bone tissueAulamedica: Source
- Effect of whole body vibration on healthy rat plasma parametersEbscohost: Source
- Biomarkers identified by urinary metabonomics for noninvasive diagnosis of nutritional ricketsPubmed:25051233
- An Attempt to Evaluate Selected Aspects of “Bone–Fat Axis” Function in Healthy Individuals and Patients With Pancreatic CancerPubMed: 26266370
- The Impact of Different Amounts of Calcium Intake on Bone Mass and Arterial Calcification in Ovariectomized RatsPubMed: 26639847
- Effects of Oryza sativa L. Aleurone Layer Extract on Bone Mineral Density and Bone-related Markers in the Ovariectomized RatArticle: Hksobf_2015_V46N2_167
- Fat and Sucrose Intake Induces Obesity‐Related Bone Metabolism Disturbances: Kinetic and Reversibility Studies in Growing and Adult RatsPubmed:26175082
- Cell-matrix signals specify bone endothelial cells during developmental osteogenesis.pubmed:28218908
- Prostaglandin-E2 Mediated Increase in Calcium and Phosphate Excretion in a Mouse Model ofDistal Nephron Salt Wasting.pubmed:27442254
- Effects of fatty acids, nutrients and whole body vibration on bone histomorphometry, mechanical properties and metabolic parameters in male rat10.22192/ijarbs.2017.04.04.018
- Chronic nicotine exposure reduces klotho expression and triggers different renal and hemodynamic responses in -haploinsufficient micePubmed:29363324
- Dietary supplementation for Santa Inês hair ewes on pasture at pre-and postpartum periods: dry matter intake, digestibility, milk production, and mineral metabolismPubmed:29931604
- CD8+ T lymphocytes enhance the anabolic effect of intermittent parathyroid hormone on cementoblastsPubmed: 31679847
- A New Therapeutic Approach Using a Calcilytic (AXT914) for Post-Surgical Hypoparathyroidism in Female RatsPubmed: 32852547
- Calciprotein particles regulate fibroblast growth factor-23 expression in osteoblasts.
- PREVENTIVE AND/OR THERAPEUTIC AGENT FOR OSTEOGENESIS IMPERFECTA AND OTHER DISEASES
- Long-Term Administration of Abacavir and Etravirine Impairs Semen Quality and Alters Redox System and Bone Metabolism in Growing Male Wistar Rats34373766
- Correction of a knock-in mouse model of acrodysostosis with gene therapy using a rAAV9-CAG-human PRKAR1A vector34599290
- Calreticulin Shortage Results in Disturbance of Calcium Storage, Mitochondrial Disease, and Kidney InjuryPubmed:35456008
- Fibroblast Growth Factor 23 (FGF 23) and intact parathyroid hormone (iPTH) as markers of mineral bone disease among Nigerians with non-diabetic kidney disease