ELISA Kit DIY Materials for Pepsin (PP) Homo sapiens (Human) Assay Kit DIY Materials

Add to Cart Distributors
Overview
Properties
  • Reagent Contents Capture Antibody, Biotin-labeled Competitor, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable SampleSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
  • Applicable PrincipleCompetitive Inhibition ELISA for Antigen Detection
  • Detectable Range2.47-200ng/mL
  • Applicable Sensitivity0.98ng/mL
Share your citation Upload your experimental result Review Leave a message
Loading...

Share a new citation as an author

Upload your experimental result

Review

Please attach serial No. on instruction manual

Contact us

Please fill in the blank.

Name*
Organization
Address
E-mail address*
Telephone
Inquiry*
Verification code* CheckCode
  • ELISA Kit DIY Materials for Pepsin (PP) Packages (Simulation)
  • ELISA Kit DIY Materials for Pepsin (PP) Packages (Simulation)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The Abs in the kit have high sensitivity and excellent specificity for detection of Pepsin (PP). No significant cross-reactivity or interference between Pepsin (PP) and analogues was observed.

Usage

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

Storage

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

Support pack

Citations

  • LEVEL OF PEPSIN AND BILE ACIDS IN THE SALIVA OF PATIENTS WITH GLOTTIS T1 CARCINOMASzd:Source
  • Higher levels of total pepsin and bile acids in the saliva as a possible risk factor for early laryngeal cancerPubmed:Pmc4362607
  • Pepsin and bile acids in saliva in patients with laryngopharyngeal reflux – a prospective comparative studyPubMed: 25516364
  • Does Positioning Affect Tracheal Aspiration of Gastric Content in Ventilated Infants?PubMed: 25313850
  • Detecting Laryngopharyngeal Reflux by Immunohistochemistry of Pepsin in the Biopsies of Vocal Fold Leukoplakiapubmed:28756936
  • Label-Free Detection of Salivary Pepsin Using Gold Nanoparticle/Polypyrrole Nanocoral Modified Screen-Printed ElectrodePubmed:29882917
  • Association between Pepsin in Bronchoalveolar Lavage Fluid and Prognosis of Chronic Fibrosing Interstitial Lung Disease
  • Pulmonary aspiration in preschool children with cystic fibrosisPubmed: 30558606
  • Association of Bile Acid and Pepsin Micro-aspiration with Chronic Obstructive Pulmonary Disease ExacerbationPubmed: 31423141
  • Optimization of Saliva Collection and Immunochromatographic Detection of Salivary Pepsin for Point-of-Care Testing of Laryngopharyngeal RefluxPubmed: 31935973
  • Presence of pepsin in laryngeal tissue and saliva in benign and malignant neoplasmsPubmed: 33103719
  • Relationship Between Pepsin Expression and Dysplasia Grade in Patients With Vocal Cord LeukoplakiaPubmed: 32692278
  • Association of pepsin and DNA damage in laryngopharyngeal reflux-related vocal fold polypsPubmed: 32889371
  • The relationship between laryngopharyngeal reflux based on pepsin value and clinical characteristics of laryngeal cancer patients
  • The Role of Salivary Pepsin in the Diagnosis of Gastroesophageal Reflux Disease (GERD) Evaluated Using High-Resolution Manometry and 24-Hour?¡­33220027
  • Pepsin properties, structure, and its accurate measurement: a narrative review
  • The Impact of Laryngopharyngeal Reflux on Occurrence and Clinical Course of Recurrent Respiratory Papillomatosis34338331
  • Bronchoalveolar lavage (BAL) amylase and pepsin levels as potential biomarkers of aspiration pneumoniaPubmed:35715334
  • Transcutaneous Electrical Acustimulation Improved the Quality of Life in Patients With Diarrhea-Irritable Bowel SyndromePubmed:35088760

Recommend products