ELISA Kit DIY Materials for Pigment Epithelium Derived Factor (PEDF) Homo sapiens (Human) Assay Kit DIY Materials

SERPINF1; EPC1; PIG35; SDF3; Serpin F1; Cell proliferation-inducing gene 35; Serpin Peptidase Inhibitor,Clade F Member 1(Alpha-2 Antiplasmin); Stromal Cell Derived Factor 3

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Overview
Properties
  • Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable SampleSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
  • Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
  • Detectable Range12.5-200ng/mL
  • Applicable Sensitivity4.9ng/mL
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  • ELISA Kit DIY Materials for Pigment Epithelium Derived Factor (PEDF) Packages (Simulation)
  • ELISA Kit DIY Materials for Pigment Epithelium Derived Factor (PEDF) Packages (Simulation)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The Abs in the kit have high sensitivity and excellent specificity for detection of Pigment Epithelium Derived Factor (PEDF). No significant cross-reactivity or interference between Pigment Epithelium Derived Factor (PEDF) and analogues was observed.

Usage

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

Storage

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

Support pack

Citations

  • A Therapeutic Strategy for Choroidal Neovascularization Based on Recruitment of Mesenchymal Stem Cells to the Sites of LesionsNature: 2010144
  • Urinary Pigment Epithelium-Derived Factor as a Marker of Diabetic NephropathyKarger: 314326
  • Increased expression of pigment epithelium-derived factor in aged mesenchymal stem cells impairs their therapeutic efficacy for attenuating myocardial infarction injuryOxfordJournals: source
  • The change of serum level of pigment epithelium-derived factor in coronary heart diseaseBmj: Source
  • Metformin Inhibits Expression and Secretion of PEDF in Adipocyte and Hepatocyte via Promoting AMPK PhosphorylationPubmed: 24288442
  • The association study of plasma levels of pigment epithelium-derived factor with acute coronary syndrome in the chinese han population.Pubmed: 24192856
  • Rosiglitazone Inhibits Expression and Secretion of PEDF in Adipose Tissue and Liver of Male SD Rats Via a PPAR-γ Independent MechanismEndocrine: Source
  • Pigment epithelium-derived factor regulates microvascular permeability through adipose triglyceride lipase in sepsisPubMed: 25700221
  • Pigment Epithelium-Derived Factor (PEDF) Protects Osteoblastic Cell Line from Glucocorticoid-Induced Apoptosis via PEDF-RPubmed:27187377
  • The Ingenious Interactions Between Macrophages and Functionally Plastic Retinal Pigment Epithelium Cellsarticleid:2582822
  • VEGF-B promotes recovery of corneal innervations and trophic functions in diabetic micepubmed:28091556
  • 色素上皮衍生因子修饰的人脐带间充质干细胞构建方法201707007
  • PEDF Reduces the Severity of Herpetic Simplex Keratitis in MicePubmed:30025136
  • 高海拔地区新生血管性青光眼不同治疗方式的疗效及对房水中PEDF, VEGF 水平的影响2018/2/201802020.pdf
  • Lactation-related changes in tissue expression of PEDF in dairy cowsPubmed:29758402
  • Correlation Between Pigment Epithelium-Derived Factor (PEDF) level and Degree of Coronary Angiography and Severity of Coronary Artery Disease in a …Pubmed:29574467
  • Role of pigment epithelium-derived factor (PEDF) on arsenic-induced neuronal apoptosisDoi: 10.1016/j.chemosphere.2018.10.100
  • Role of Pigment Epithelium-Derived Factor in Arsenic-Induced Vascular Endothelial Dysfunction in a Rat ModelPubmed: 30392020
  • Increased levels of serum pigment epithelium-derived factor aggravate proteinuria via induction of podocyte actin rearrangementPubmed: 30536192
  • A novel xeno-free culture system for human retinal pigment epithelium cellsPubmed: 31024807
  • Pigment Epithelial‐Derived Factor Deficiency Accelerates Atherosclerosis Development via Promoting Endothelial Fatty Acid Uptake in Mice With HyperlipidemiaPubmed: 31711388
  • Pigment epithelium-derived factor (PEDF) ameliorates arsenic-induced vascular endothelial dysfunction in rats and toxicity in endothelial EA. hy926 cellsPubmed: 32315827
  • The Expression Levels and Cellular Localization of Pigment Epithelium Derived Factor (PEDF) in Mouse Testis: Its Possible Involvement in the Differentiation of Spermatogonial Cells33498962
  • Long non-coding RNA Rian promotes the expression of tight junction proteins in endothelial cells by regulating perivascular-resident macrophage-like melanocytes ¡­33768511

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