ELISA Kit DIY Materials for Plasminogen (Plg) Rattus norvegicus (Rat) Assay Kit DIY Materials

PL; Plasmin; Activation peptide; Angiostatin

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Overview
Properties
  • Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable Sampleserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
  • Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
  • Detectable Range15.6-1,000ng/mL
  • Applicable Sensitivity6.9ng/mL
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  • ELISA Kit DIY Materials for Plasminogen (Plg) Packages (Simulation)
  • ELISA Kit DIY Materials for Plasminogen (Plg) Packages (Simulation)
  • KSB236Ra01.jpg Picture
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The Abs in the kit have high sensitivity and excellent specificity for detection of Plasminogen (Plg).No significant cross-reactivity or interference between Plasminogen (Plg) and analogues was observed.

Usage

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

Storage

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

Support pack

Citations

  • The Anticoagulant Effect of PGI2S and tPA in Transgenic Umbilical Vein Endothelial Cells Is Linked to Up-Regulation of PKA and PKCMdpi: Source
  • Improved milk production through PG-PL system by provision of in-house shelter management in lactating Murrah buffaloes during winter season.pubmed:28084661
  • iTRAQ-Based Quantitative Proteomics Reveals the New Evidence Base for Traumatic Brain Injury Treated with Targeted Temperature Managementpubmed:29247448
  • The influence of a modified micro-environment on stress and milk production through the plasminogen-plasmin system in Murrah buffaloes during the hot-humid …10.24099:vet.arhiv.170113
  • Reducing antigenicity of ¦Â-lactoglobulin, probiotic properties and safety evaluation of Lactobacillus plantarum AHQ-14 and Lactobacillus bulgaricus BD0390
  • Antigenicity and Safety Evaluation of Lactiplantibacillus plantarum 7-2 Screened to Reduce α-Casein AntigenPubmed:35010214

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