ELISA Kit DIY Materials for Procalcitonin (PCT) Chicken (Gallus) Assay Kit DIY Materials

Pro-Calcitonin

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Overview
Properties
  • Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable SampleSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
  • Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
  • Detectable Range31.2-2000pg/mL
  • Applicable Sensitivity12.7pg/mL
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  • ELISA Kit DIY Materials for Procalcitonin (PCT) Packages (Simulation)
  • ELISA Kit DIY Materials for Procalcitonin (PCT) Packages (Simulation)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The Abs in the kit have high sensitivity and excellent specificity for detection of Procalcitonin (PCT). No significant cross-reactivity or interference between Procalcitonin (PCT) and analogues was observed.

Usage

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

Storage

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

Support pack

Citations

  • Evaluation of neutrophilic CD64, interleukin 10 and procalcitonin as diagnostic markers of early- and late-onset neonatal sepsisPubMed: 20085423
  • Effects of Berberine Against Radiation-Induced Intestinal Injury in MicePubMed: 20637981
  • Diagnostic and prognostic value of procalcitonin and phosphorus in acute mesenteric ischemiaPubMed: 21935794
  • Serum procalcitonin and its relation to the outcome of ventilator-associated pneumoniain childrenEssbronchology: Source
  • The Assessment of Vitamin D, Antimicrobial Peptides and Procalcitonin in BronchiectasisWaikato:Source
  • Evaluation of the Diagnostic Utility of Fc Gamma Receptor Type 1 (nCD64) and Procalcitonin in Comparison with Conventional Methods for Diagnosis of Early Onset Neonatal SepsisEjmm:Source
  • Efficacy of measuring procalcitonin levels in determination of prognosis and early diagnosis of bacterial resistance in sepsisPubmed:25657488
  • The use and value of procalcitonin in solid organ transplantationPubMed: 25996831
  • Diagnostic Value of Procalcitonin Levels in Acute Mesenteric IschemiaPubMed: 26185718
  • Macrocalcitonin is a novel pitfall in the routine of serum calcitonin immunoassayPubMed: 26647152
  • Procalcitonin and Pentraxin-3: Current biomarkers in inflammation in white coat hypertensionPubMed: 26040439
  • Variation of Circulating Inflammatory Mediators in Staphylococcus aureus and Escherichia coli BloodstreamInfection.Pubmed:26772168
  • Consistency and pathophysiological characterization of a rat polymicrobial sepsis model via the improved cecal ligation and puncture surgeryPubmed:26802602
  • Early prediction of intestinal mucosal barrier function impairment by elevated serum procalcitonin in rats with severe acute pancreatitis.Pubmed:26804005
  • Risk Factors of Acute Pancreatitis in Oral Double Balloon Enteroscopy.pubmed:27638962
  • Changes in inflammatory mediators as a result of intermittent hypoxia in obstructive sleep apnea syndromepubmed:28960823
  • INTERLEUKIN-1 RECEPTOR 2: A NEW BIOMARKER FOR SEPSIS DIAGNOSIS AND GRAM-NEGATIVE/GRAM-POSITIVE BACTERIAL DIFFERENTIATIONpubmed:27984536
  • Hepatic Macrophages are the Cell Source of Hepatic Procalcitonin in Acute Liver FailurePubmed:29913443
  • Inflammatory mediators in the diagnosis and treatment of acute pancreatitis: pentraxin-3, procalcitonin and myeloperoxidasePubmed:29593801
  • Expression pattern of soluble triggering receptor expressed on myeloid cells-1 in mice with Acinetobacter baumannii colonization and infection in the lungPubmed:29707313
  • Procalcitonin and C-reactive protein as peripheral inflammatory markers in antipsychotic drug-free schizophrenia patientsPubmed:29723719
  • The predictive value of joint detection of serum amyloid protein A, PCT, and Hs-CRP in the diagnosis and efficacy of neonatal septicemia.Pubmed: 31298341
  • Modestly Elevated Serum Procalcitonin Levels in Patients with Rheumatoid Arthritis Free of Active InfectionPubmed: 33080909
  • Reevaluation of Lung Injury in TNF-Induced Shock: The Role of the Acid SphingomyelinasePubmed: 32410851
  • Early label-free analysis of mitochondrial redox states by Raman spectroscopy predicts septic outcomes
  • Moesin Is a Novel Biomarker of Endothelial Injury in Sepsis33628853
  • Circulating nuclear factor-kappa B mediates cancer-associated inflammation in human breast and colon cancer33776564
  • Original contribution: sleeve gastrectomy reduces soluble lectin-like oxidized low-density lipoprotein receptor-1 (sLOX-1) levels in patients with morbid obesityPubmed:35044516
  • Procalcitonin is expressed in osteoblasts and limits bone resorption through inhibition of macrophage migration during intermittent PTH treatmentPubmed:35087025

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