ELISA Kit DIY Materials for Procollagen II C-Terminal Propeptide (PIICP) Mus musculus (Mouse) Assay Kit DIY Materials

P2CP; C-Propeptide Of Type II Procollagen; Procollagen II Carboxy Terminal Propeptide

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Overview
Properties
  • Reagent Contents Capture Antibody, Biotin-labeled Competitor, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable SampleSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
  • Applicable PrincipleCompetitive Inhibition ELISA for Antigen Detection
  • Detectable Range3.70-300ng/mL
  • Applicable Sensitivity1.49ng/mL
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  • ELISA Kit DIY Materials for Procollagen II C-Terminal Propeptide (PIICP) Packages (Simulation)
  • ELISA Kit DIY Materials for Procollagen II C-Terminal Propeptide (PIICP) Packages (Simulation)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The Abs in the kit have high sensitivity and excellent specificity for detection of Procollagen II C-Terminal Propeptide (PIICP). No significant cross-reactivity or interference between Procollagen II C-Terminal Propeptide (PIICP) and analogues was observed.

Usage

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

Storage

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

Support pack

Citations

  • Evaluation of the effect of N-acetyl-glucosamine administration on biomarkers for cartilage metabolism in healthy individuals without symptoms of arthritis: A …10.3892
  • Evaluation of the effect of administering N-acetyl-glucosamine-containing green tea supplement on biomarkers for cartilage metabolism in healthy individuals without symptoms of arthritis: a randomized double-blind placebo-controlled clinical study309
  • Effect of N-acetylglucosamine administration on cartilage metabolism and safety in healthy subjects without symptoms of arthritis: A case reportpubmed:28413518
  • Evaluation of the effect of salmon nasal proteoglycan on biomarkers for cartilage metabolism in individuals with knee joint discomfort: A randomized double‑blind placebo‑controlled clinical studyetm:14
  • Evaluation of the efficacy of Ajuga decumbens extract supplement in individuals with knee discomfort associated with physical activity: A randomized, double‑blind, placebo‑controlled study10.3892/etm.2017.5064
  • Evaluation of the chondroprotective action of N‑acetylglucosamine in a rat experimental osteoarthritis pubmed:28912864
  • Evaluation of the effect of N-acetyl-glucosamine administration on biomarkers for cartilage metabolism in healthy individuals: a randomized double-blind placebo-controlled clinical studyview/366
  • No effects of hyperosmolar culture medium on tissue regeneration by human degenerated nucleus pulposus cells despite upregulation extracellular matrix genesfulltext:2018/03010
  • Fibulin-3 and other cartilage metabolism biomarkers in relationship to calprotectin (MRP8/14) and disease activity in rheumatoid arthritis patients treated with …68362.pdf
  • サケ鼻軟骨由来プロテオグリカン摂取による関節保護効果
  • No Effects of Hyperosmolar Culture Medium on Tissue Regeneration by Human DegeneratedPubmed: 25856264
  • Effectiveness of collagen supplementation on pain scores in healthy individuals with self-reported knee pain; A randomized controlled trialPubmed: 31990581
  • Bone Morphogenetic Proteins for Nucleus Pulposus RegenerationPubmed: 32295299
  • Concerted Actions by PIICP, CTXII, and TNF-¦Á in Patients with Juvenile Idiopathic Arthritis33924892

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