ELISA Kit DIY Materials for Progesterone (PG) Pan-species (General) Assay Kit DIY Materials

P4; Pregn-4-Ene-3,20-Dione

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Overview
Properties
  • Reagent Contents Capture Antibody, Biotin-labeled Competitor, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable Sampleserum, plasma and other biological fluids
  • Applicable PrincipleCompetitive Inhibition ELISA for Antigen Detection
  • Detectable Range1.23-100ng/mL
  • Applicable Sensitivity0.47ng/mL
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  • ELISA Kit DIY Materials for Progesterone (PG) Packages (Simulation)
  • ELISA Kit DIY Materials for Progesterone (PG) Packages (Simulation)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The Abs in the kit have high sensitivity and excellent specificity for detection of Progesterone (PG). No significant cross-reactivity or interference between Progesterone (PG) and analogues was observed.

Usage

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

Storage

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

Support pack

Citations

  • Growth differential factor-9 inhibits testosterone production in mouse theca interstitial cells Pubmed: 23954356
  • Prevention of preterm birth by progestational agents: what are the molecular mechanisms? Pubmed: 23433326
  • The role of Sep (O-phosphoserine) tRNA: Sec (selenocysteine) synthase (SEPSECS) in proliferation, apoptosis and hormone secretion of trophoblast cellsPubmed: 23966103
  • Neutrophil gene dynamics and plasma cytokine levels in dairy cattle during peri-implantation periodPubmed:27090626
  • Assessment of ADMA, estradiol, and progesterone in severe preeclampsiaPubmed:27152507
  • The Reproductive Toxicity of CdSe/ZnS Quantum Dots on the in vivo Ovarian Function and in vitro Fertilizationpubmed:27876896
  • Molecular characterization of insulin resistance and glycolytic metabolism in the rat uteruspubmed:27461373
  • Metformin Ameliorates Uterine Defects in a Rat Model of Polycystic Ovary Syndrome.pubmed:28336389
  • Exposure of pregnant mice to perfluorobutanesulfonate causes hypothyroxinemia and developmental abnormalities in female offspringpubmed:27803384
  • Middle-Aged Diabetic Females and Males Present Distinct Susceptibility to Alzheimer Disease-like Pathology.pubmed:27730513
  • Exposure of pregnant mice to triclosan impairs placental development and nutrient transport.pubmed:28322267
  • Acute administration of oestradiol or progesterone in a spinal cord ischaemia–reperfusion model in ratspubmed:29049608
  • Exposure of Pregnant Mice to Triclosan Causes Insulin Resistance via Thyroxine Reductionpubmed:28973666
  • The anorectic agent, lorcaserin, disturbs estrous cyclicity and produces endometrial hyperplasia without affecting ovarian population in female ratspubmed:28599864
  • Progesterone attenuates hypertension and autoantibody levels to the angiotensin II type 1 receptor in response to elevated cadmium during pregnancy10.1016:j.placenta.2017.12.004
  • The biocompatibility studies of polymer dots on pregnant mice and fetusespubmed:29071192
  • Beneficial effects of Heqi san on rat model of polycystic ovary syndrome through the PI3K/AKT pathwaypubmed:29020999
  • Impact of Perfluorooctane Sulfonate on Reproductive Ability of Female Mice through Suppression of Estrogen Receptor a-Activated Kisspeptin Neurons10.1093:toxsci
  • Impact of Triclosan on Female Reproduction through Reducing Thyroid Hormones to Suppress Hypothalamic Kisspeptin Neurons in MicePubmed:29403355
  • Up-Regulation of Long Noncoding RNA SRA Promotes Cell Growth, Inhibits Cell Apoptosis, and Induces Secretion of Estradiol and Progesterone in Ovarian …Pubmed:29674607
  • Prostaglandin E2 triggers cytochrome P450 17α hydroxylase overexpression via signal transducer and activator of transcription 3 phosphorylation and promotes …10.3892:ol.2018.9165
  • Crocetin attenuates DHT-induced polycystic ovary syndrome in mice via revising kisspeptin neuronsDoi: 10.1016/j.biopha.2018.08.135
  • Uterine progesterone signaling is a target for metformin therapy in polycystic ovary syndromePubmed: 29535146
  • Endometrial mesenchymal stem cells isolated from menstrual blood repaired epirubicin-induced damage to human ovarian granulosa cells by inhibiting the …Pubmed: 30606243
  • Dihydrotestosterone synthesis in the sheep corpus luteum and its potential mechanism in luteal regressionPubmed: 30671954
  • Tanshinone IIA attenuates estradiol‑induced polycystic ovarian syndrome in mice by ameliorating FSHR expression in the ovary
  • Reduced serotonin impairs long-term depression in basolateral amygdala complex and causes anxiety-like behaviors in a mouse model of perimenopausePubmed: 31377402
  • Evidence that downregulation of Wilms' tumor 1 (WT1) is involved in cortical stromal cell differentiation into theca cells in adult bovine ovariesPubmed: 31490589
  • Baicalin ameliorates polycystic ovary syndrome through AMP-activated protein kinasePubmed: 31722718
  • Exposure of adult mice to perfluorobutanesulfonate impacts ovarian functions through hypothyroxinemia leading to down-regulation of Akt-mTOR signalingPubmed: 31809938
  • An antibody-aptamer sandwich cathodic photoelectrochemical biosensor for the detection of progesteronePubmed: 32339148
  • Serum Progesterone and Testosterone Levels in Schizophrenia Patients at Different Stages of TreatmentPubmed: 33159671
  • Prenatal exposure of female mice to perfluorononanoic acid delays pubertal activation of the reproductive endocrine axis through enhanced hepatic FGF21 …Pubmed: 33131727
  • MiRNAs expression profiling of rat ovaries displaying PCOS with insulin resistancePubmed: 32757043
  • Guoqi Liu1, Juan Li2, 3, Peng Cui2, Susanne Lager5, Amanda Nancy Sferruzzi-Perri6, Wei Li1, Xiao-ke Wu1, Yanhua Han1, Mats Brännström7, Linus R Shao2 …Pubmed: 31206177
  • Pathophysiological Changes in Female Rats with Estrous Cycle Disorder Induced by Long-Term Heat StressPubmed: 32685488
  • Wilms' tumor (WT1)(+/-KTS) variants decreases the progesterone secretion of bovine ovarian theca cellsPubmed: 32739762
  • Implantation associated changes in expression profile of indoleamine-2, 3-dioxygenase 1, Th1-Th2 cytokines and interferon-stimulated genes on neutrophils and …Pubmed: 32846354
  • Therapeutic effects of organic zinc on reproductive hormones, insulin resistance and mTOR expression as a novel component in a rat model of PCOS
  • Improving reproductive efficiency through the supplementation of mustard oil, poly-herbal mixture and butyric acid during the periparturient period in Sahiwal …
  • A preliminary study on the mechanism of the neurosteroid-mediated ionotropic receptor dysfunction in neurodevelopmental toxicity induced by?¡­33862428
  • MiR-145 regulates steroidogenesis in mouse primary granulosa cells through targeting Crkl34273377
  • Effects of a Nanoencapsulated Moringa Leaf Ethanolic Extract on the Physiology, Metabolism and Reproductive Performance of Rabbit Does during Summer34439574
  • Zika virus infection in the ovary induces continuously elevated progesterone level and compromises conception in interferon α/β receptor-deficient mice34730391

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